DNA Fingerprinting by ISSR Markers for 17 Blueberries

ZHENG Shan; ZHANG Li-jie; XIE Li-xue; ZHANG Xiao-yan; LI Tao

doi:10.19303/j.issn.1008-0384.2014.12.008

Volume 29 Issue 12

Dec. 2014

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ZHENG Shan, ZHANG Li-jie, XIE Li-xue, ZHANG Xiao-yan, LI Tao. DNA Fingerprinting by ISSR Markers for 17 Blueberries[J]. Fujian Journal of Agricultural Sciences, 2014, 29(12): 1198-1201. doi: 10.19303/j.issn.1008-0384.2014.12.008

Citation:

ZHENG Shan, ZHANG Li-jie, XIE Li-xue, ZHANG Xiao-yan, LI Tao. DNA Fingerprinting by ISSR Markers for 17 Blueberries[J]. Fujian Journal of Agricultural Sciences, 2014, 29(12): 1198-1201. doi: 10.19303/j.issn.1008-0384.2014.12.008

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DNA Fingerprinting by ISSR Markers for 17 Blueberries

doi: 10.19303/j.issn.1008-0384.2014.12.008

Fruit Research Institute,Fujian Academy of Agricultural Sciences

Received Date: 2014-10-25
Publish Date: 2014-12-18

Abstract

Abstract

Inter-simple sequence repeat (ISSR) analysis was applied for the determination of the genetic diversity, as well as the establishment of the DNA fingerprints, for 17 selected blueberry cultivars.Ten out of 60 ISSR primers were chosen for the study.Cluster analysis revealed that the genetic similarity coefficients of the 17 samples ranged from 0.66 to 0.86.Using the coefficient threshold of 0.69, 4classifications were formed for the blueberries.They could be positively distinguished by 2ISSR primers (i.e., UBC825 and UBC862) , and used to obtain the digital DNA fingerprints.
- blueberry,
- ISSR,
- fingerprint,
- genetic diversity

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References(14)

References

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