Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)

FANG Zhi-zhen; ZHOU Dan-rong; YE Xin-fu; JIANG Cui-cui; PAN Shao-lin

doi:10.19303/j.issn.1008-0384.2015.02.006

Volume 30 Issue 2

Feb. 2015

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(2): 136-140

FANG Zhi-zhen, ZHOU Dan-rong, YE Xin-fu, JIANG Cui-cui, PAN Shao-lin. Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 136-140. doi: 10.19303/j.issn.1008-0384.2015.02.006

Citation:

FANG Zhi-zhen, ZHOU Dan-rong, YE Xin-fu, JIANG Cui-cui, PAN Shao-lin. Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)[J]. Fujian Journal of Agricultural Sciences, 2015, 30(2): 136-140. doi: 10.19303/j.issn.1008-0384.2015.02.006

Citation:

PDF( 1380 KB)

Cloning and Bioinformatic Analysis of PsACO1 gene from Furong Plum(Prunus salicina Lindl.)

doi: 10.19303/j.issn.1008-0384.2015.02.006

Fruit Research Institute,Fujian Academy of Agricultural Sciences

Funds:

福建省农业科学院果树研究所,福建福州,350013

Received Date: 2015-01-02
Publish Date: 2015-02-18

Abstract

Abstract

A 1309 bp full length cDNA sequence of PsACO1 gene were isolated from Furong plum(Prunus salicina Lindl.)through RT-PCR and RACE technology.The isolated gene contains a 957 bp open reading frame and encodes a protein of 319 amino acids with isoelectric point of 5.28 and molecular weight of 36.24 kD.PsACO1protein contains characteristic domains of ACO proteins.The phylogenetic analysis results showed that PsACO1 has close corelation with ACO protein fromPrunus persica,Prunus mume,Eriobotryajaponica,Malus domestica and Prunus domestica.
- Plum,
- ACO,
- gene cloning,
- bioinformatics