Study on Enhancing Bacterial Blight Resistance of Hybrid Rice Restorer Lines through Marker Assisted Selection of the Xa23 Gene

YANG De-wei; YE Ning; YE Xin-fu; ZHENG Xiang-hua; CHENG Chao-ping; LU Li-bin; HUANG Feng-huang

doi:10.19303/j.issn.1008-0384.2015.04.008

Volume 30 Issue 4

Apr. 2015

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Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(4): 351-356

YANG De-wei, YE Ning, YE Xin-fu, ZHENG Xiang-hua, CHENG Chao-ping, LU Li-bin, HUANG Feng-huang. Study on Enhancing Bacterial Blight Resistance of Hybrid Rice Restorer Lines through Marker Assisted Selection of the Xa23 Gene[J]. Fujian Journal of Agricultural Sciences, 2015, 30(4): 351-356. doi: 10.19303/j.issn.1008-0384.2015.04.008

Citation:

YANG De-wei, YE Ning, YE Xin-fu, ZHENG Xiang-hua, CHENG Chao-ping, LU Li-bin, HUANG Feng-huang. Study on Enhancing Bacterial Blight Resistance of Hybrid Rice Restorer Lines through Marker Assisted Selection of the Xa23 Gene[J]. Fujian Journal of Agricultural Sciences, 2015, 30(4): 351-356. doi: 10.19303/j.issn.1008-0384.2015.04.008

Citation:

YANG De-wei, YE Ning, YE Xin-fu, ZHENG Xiang-hua, CHENG Chao-ping, LU Li-bin, HUANG Feng-huang. Study on Enhancing Bacterial Blight Resistance of Hybrid Rice Restorer Lines through Marker Assisted Selection of the Xa23 Gene[J]. Fujian Journal of Agricultural Sciences, 2015, 30(4): 351-356. doi: 10.19303/j.issn.1008-0384.2015.04.008

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Study on Enhancing Bacterial Blight Resistance of Hybrid Rice Restorer Lines through Marker Assisted Selection of the Xa23 Gene

doi: 10.19303/j.issn.1008-0384.2015.04.008

Institute of Rice,Fujian Academy of Agricultural Sciences

Received Date: 2015-01-02
Publish Date: 2015-04-18

Abstract

Abstract

The bacterial blight is one of the major diseases in rice.One effective control measure for bacterial blight is to breed resistance cultivars through molecular marker closely linked to the resistance genes.The rice bacterial blight resistance gene Xa23,carried by the donor parent IRBB23,was introduced into the restorer line Dongnanhui 012'by regular and back crossing and molecular(marker assisted selection.This research was carried out in low generation without resistance using molecular marker assisted selection with marker C189 closely linked to Xa23 gene,and in high and stable strains using seedling population identification technology.A series of precocious restore lines with homozygous target genes Xa23 and stable good agronomic characters were obtained through testing the flanking marker C189.The eight lines were subject to bacterial blight disease inoculation at the booting stages,and results showed that seven lines were resistant to the most destructive Xoostrain PXO 99(P6).Meanwhile,we also analyzed the main agronomic traits of the seven homozygous lines.These results clearly suggested that the marker C189 linked to Xa23 could be conveniently and effectively used in the bacterial blight resistance breeding programs of rice.
- rice,
- restore line,
- bacterial blight,
- Xa23 gene,
- marker assisted selection

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References(28)

References

[1]	MEW T W.Curent status and future prospects of research on bacterial blight of rice[J].Annu Rev Phytopathol,1987,25:359-382.
[2]	秦钢,李道远,李杨瑞,等.水稻白叶枯病抗性基因Xa23导入效应分析[J].西南农业学报,2008,21(1):71-74.
[3]	曾列先,成太辉,朱小源,等.广东首个抗白叶枯病强毒菌系V型菌水稻品种白香占的选育[J].广东农业科学,2009,(5):19-28.
[4]	易懋升,丁效华,张泽民,等.水稻抗白叶枯病恢复系的分子育种[J].华南农业大学学报,2006,27(2):l-4.
[5]	曹立勇,庄杰云,占小登,等.抗白叶枯病杂交水稻的分子标记辅助育种[J].中国水稻科学,2003,17(2):184-186.
[6]	邓其明,王世全,郑爱萍,等.利用分子标记辅助育种技术选育高抗白叶枯病恢复系[J].中国水稻科学,2006,20(2):153-158.
[7]	郑家团,涂诗航,张建福,等.含白叶枯病抗性基因Xa23水稻恢复系的分子标记辅助选育[J].中国水稻科学,2009,23(4):437-439.
[8]	王建军,朱旭东,王林友,等.水稻类病变突变体lrd40的抗性与细胞学分析[J].中国水稻科学,2005,19(2):111-116.
[9]	王春连,戚华雄,潘海军,等.水稻抗白叶枯病基因Xa23的EST标记及其在分子育种上的利用[J].中国农业科学,2005,38(10):1996-2001.
[10]	LANDER E S,GREEN P,ABRAHAMSON J,et al.Corrigendum to“Mapmaker:an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations”[J].Genomics,1987,1(2):174-181.
[11]	王昊龙,韩俊杰,李淼淼,等.功能标记的开发及在禾谷类作物中的应用[J].核农学报,2014,28(11):1963-1971.
[12]	牟少亮,蔡金森,严雁,等.水稻Pi-ta启动子的克隆及其功能分析[J].核农学报,2013,27(12):1803-1808.
[13]	季芝娟,富昊伟,马良勇,等.带显性抗白叶枯病基因Xa23的特早熟籼型不育系中嘉A[J].中国稻米,2007,(30):26-27.
[14]	刘毅,王加红,黎良通,等.分子标记辅助选择Xa23基因改良节水抗旱稻亲本的白叶枯病抗性研究[J].上海农业学报,2014,30(1):13-16.
[15]	李进波,王春莲,夏明元,等.分子标记辅助选择Xa23基因培育杂交稻抗白叶枯病恢复系[J].作物学报,2006,32(10):1423-1429.
[16]	于洁,王耀雯,马文清,等.水稻抗白叶枯病基因Xa23群体的MAS育种研究[J].华南农业大学学报,2010,31(4):1-5.
[17]	范宏环,王林友,张礼霞,等.通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系[J].中国水稻科学,2011,25(3):331-334.
[18]	潘晓飚,陈凯,张强,等.分子标记辅助选育水稻抗白叶枯病和稻瘟病多基因聚合恢复系[J].作物学报,2013,39(9):1582-1593.
[19]	闫成业,仲雪婷,GANDEKA MAMADOU,等.分子标记辅助选择改良杂交水稻组合Q优6号的白叶枯病抗性[J].分子植物育种,2013,11(6):680-687.
[20]	肖武名,孙大元,王慧,等.分子标记选育稻瘟病和白叶枯病双抗种质[J].华北农学报,2014,29(1):203-207.
[21]	闫成业刘艳牟同敏.分子标记辅助选择改良杂交水稻金优207的白叶枯病抗性[J].中国水稻科学,2013,27(4):365-372.
[22]	SUNDARAM R M,CHATTERJEE S,OLIVA R,et al.Update on Bacterial Blight of Rice:Fourth International Conference on Bacterial Blight[J].Rice,2014,(7):12.
[23]	郑燕梅,吴春珠,刘玉芹,等.分子标记辅助选择改良Ⅱ-32B白叶枯病抗性[J].热带作物学报,2013,34(12):2458-2462.
[24]	王涛,王长春,胡海涛,等.6个已克隆水稻白叶枯病抗性基因及其作用机理[J].浙江农业学报,2011,26(3):1282-1289.
[25]	马良勇,李西明,季芝娟,等.四抗水稻品种中组14的选育与利用[J].分子植物育种,2010,8(6):1208-1213.
[26]	丁伦友.高抗白叶枯病系列杂交稻的选育[J].杂交水稻,2005,20(1):11-14.
[27]	夏春,陈红旗,朱旭东.水稻白叶枯病抗性基因的鉴定、定位、克隆与育种应用[J].分子植物育种,2012,10(6):761-771.
[28]	WANG C L,FAN Y L,ZHENG C K,et al.Toxicity and Control Efficacy of Spirotetramat Mixed with Thiamethoxam on Bemisia tabaci and its Security to Ornamental Chrysanthemum Cultivar[J].Molecular Genetics and Genomics.2014,289(5):745-753.