Analysis on Nigericin from Brevibacillus choshinensis FJAT-10008

ZHU Yu-jing; LIU Bo; ZHENG Mei-xia; CHEN Zheng; SHI Huai

doi:10.19303/j.issn.1008-0384.2015.10.006

Volume 30 Issue 10

Oct. 2015

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ZHU Yu-jing, LIU Bo, ZHENG Mei-xia, CHEN Zheng, SHI Huai. Analysis on Nigericin from Brevibacillus choshinensis FJAT-10008[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 948-953. doi: 10.19303/j.issn.1008-0384.2015.10.006

Citation:

ZHU Yu-jing, LIU Bo, ZHENG Mei-xia, CHEN Zheng, SHI Huai. Analysis on Nigericin from Brevibacillus choshinensis FJAT-10008[J]. Fujian Journal of Agricultural Sciences, 2015, 30(10): 948-953. doi: 10.19303/j.issn.1008-0384.2015.10.006

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Analysis on Nigericin from Brevibacillus choshinensis FJAT-10008

doi: 10.19303/j.issn.1008-0384.2015.10.006

Agricultural Bioresources Research Institute，Fujian Academy of Agricultural Sciences，Fuzhou，Fujian 350003，China

Received Date: 2015-08-06
Publish Date: 2015-10-18

Abstract

Abstract

Extracellular metabolites of Brevibacillus choshinensis FJAT-10008 were analyzed by liquid chromatographyhybrid quadrupole time-of-flight mass spectrometry and Nigericin from FJAT-10008 was tested. Molecular Feature Extraction(MFE)and database retrieval were applied to access the information of overall metabolites of B.choshinensis.As a result,1246 compounds were detected and 206 of them were identified.The match score of Nigericin was 92.12 and its relative content was 1.45%.The retention time of Nigericin was about 28.7017 min,and its accurate mass was724.4679.The results provided a theory basis for development and utilization of polyether antibiotics fromB.choshinensis.

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References(28)

References

[1]	TAKATSU T,OHTSUKI M,MURAMATSU A,et al.Reblastatin,a novel benzenoid ansamycin-type cell cycle inhibitor[J].Journal of Antibiotics,2000,53(11):1310-1312.
[2]	GUFFANTIA A,Davidson L F,Mann TM,et al.Nigericininduced death of an acidophilic bacterium[J].Journal of General Microbiology,1979,114(1):201-206.
[3]	MATES S M,EISENKERG E,MANDEL L J,et al.Membrane potential and gentamycin uptake in Staphylococcus aureus[J].Proceedings of the National Academy of Sciences,1982,79(21):6693-6697.
[4]	SEIBERT G,RAETHER W,WINKLER I,et al.Mixture of ciclopirox and derivatives with synergistic antimycotic and antiviral activity.Eur Pat Appl:EP 358177[P],1990-03-14.
[5]	HASUDA K,LEE C,TANNOCK I F.Antitumor activity of nigericin and 5-(N-ethyl-N-isopropy)amiloride:an approach to therapy based on cellu-lar acidification and the inhibition of regulation of intracellular pH[J].Oncology Research,1994,6(6):259-268.
[6]	HAN J W,SHIM D W,SHIN W Y,et al.Anti-inflammatory effect of emodin via attenuation of NLRP3 inflammasome activation[J].International Journal of Molecular Sciences,2015,16(4):8102-8109.
[7]	KONG X,SU F,ZHANG L,et al.A Highly Selective Mitochondria-Targeting Fluorescent K+Sensor[J].Angewandte Chemie International Edition.2015,54(41):12053-12057.
[8]	ZHOU H M,DONG T T,WANG L L,et al.Suppression of colorectal cancer metastasis by nigericin through inhibition of epithelial-mesenchymal transition[J].World Journal of Gastroenterology,2012,18(21):2640-2648.
[9]	肖琳,黄为一.吸水链霉菌NND-52中小组分抗生素M2的结构鉴定及生物活性[J].中国生化药物杂志,2002,23(1):7-10.
[10]	HARVEY B M,MIRONENKO T,SUN Y,et al.Insights into polyether biosynthesis from analysis of the nigericin biosynthetic gene cluster in Streptomyces sp.DSM4137[J].Chemistry and Biology,2007,14(6):703-714
[11]	肖琳,黄为一.尼日利亚菌素高产菌株的选育[J].微生物学通报,2002,29(4):59-62.
[12]	牛四文,李苏梅,田新朋,等.海洋放线菌Streptomyces sp.SCSIO 1934中次生代谢产物的分离和鉴定[J].中国中药杂志,2011,36(13):1763-1768.
[13]	卓锦明,徐兰,林永勉,等.抗MRSA抗生素FW99501的分离纯化、结构鉴定和生物学活性[J].海峡药学,2006,18(2):153-156.
[14]	杨慧林,张志斌,颜日明,等.东乡野生稻内生放线菌Streptomyces sp.PRh5的全基因组测序及序列分析[J].微生物学通报,2015,42(4):801-809.
[15]	郭丽清.苏云金芽胞杆菌中脂肽类抗生素Iturins的研究[D].福建:福建农林大学,2010.
[16]	TANIO M,KUSUNOKI H,KOHNO T.H,C and 5 N backbone resonance assignments of the monomeric human Mficolin fibrinogen-like domain secreted by Brevibacillus choshinensis[J].Biomolecular NMR Assignments,2014,8(1):207-211.
[17]	CHENG Y M,LU M T,YEH C M.Functional expression of recombinant human trefoil factor 1 by Escherichia coli and Brevibacillus choshinensis[J].BMC Biotechnology,2015,20(15):32.
[18]	MIZUKAMI M,TOKUNAGA H,ONISHI H,et al.Highly efficient production of VHH antibody fragments in Brevibacillus choshinensis expression system[J].Protein Expression and Purification,2014,5(105):23-32.
[19]	MU T,LIANG W,JU Y,et al.Efficient soluble expression of secreted matrix metalloproteinase 26 in Brevibacillus choshinensis[J].Protein Expression and Purification,2013,91(2):125-33.
[20]	TOKUNAGA M,MIZUKAMI M,YAMASAKI K.Secretory production of single-chain antibody(scFv)in Brevibacillus choshinensis using novel fusion partner[J].Applied Microbiology and Biotechnology,2013,97(19):8569-8580.
[21]	TANIO M,TANAKA T,KOHNO T.15Nisotope labeling of a protein secreted by Brevibacillus choshinensis for NMR study[J].Analytical Biochemistry,2008,373(1):164-166.
[22]	TANIO M,TANAKA R,TANAKA T,et al.Amino acidselective isotope labeling of proteins for nuclear magnetic resonance study:proteins secreted by Brevibacillus choshinensis[J].Analytical Biochemistry,2009,386(2):156-160.
[23]	HOME I,WILLIAMS M,SUTHERLAND T D,et al.A Brevibacillus choshinensis system that secretes cytoplasmic proteins[J].Journal of Molecular Microbiology and Biotechnology,2004,8(2):81-90.
[24]	MAEHASHI K,MATANO M,SAITO M,et al.Extracellular production of riboflavin-binding protein,a potential bitter inhibitor,by Brevibacillus choshinensis[J].Protein Expression and Purification,2010,71(1):85-90.
[25]	TANAKA R,KOSUGI K,MIZUKAMI M,et al.Expression and purification of thioredoxin(TrxA)and thioredoxin reductase(TrxB)from Brevibacillus choshinensis[J].Protein Expression and Purification,2004,37(2):385-391.
[26]	CHRISTOFI N,IVSHINA I B.Microbial surfactants and their use in field studies of soil remediation[J].Journa of Applied Microbiology,2002,93(6):915-929.
[27]	BERRADA R,DAUPHIN G,David L.Epinigericin,a new polyether carboxylic antibiotic.Structural determination by 2D NMR methods[J].Journal of Organic Chemistry,1987,52(12):2388-2391.
[28]	黄维其.多醚类抗生素的结构和生物合成[J].国外药学,1988,(3):237-238.