NS1-ELISA for Antibody Detection on Avian Tembusu Virus

WAN Chun-he; PAN Yi-zhe; FU Qiu-ling; CHEN Zhen; CHEN Cui-teng; FU Guang-hua; CHEN Hong-mei; CHENG Long-fei; SHI Shao-hua; HUANG Yu

doi:10.19303/j.issn.1008-0384.2015.11.001

Volume 30 Issue 11

Nov. 2015

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(11): 1027-1031

WAN Chun-he, PAN Yi-zhe, FU Qiu-ling, CHEN Zhen, CHEN Cui-teng, FU Guang-hua, CHEN Hong-mei, CHENG Long-fei, SHI Shao-hua, HUANG Yu. NS1-ELISA for Antibody Detection on Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1027-1031. doi: 10.19303/j.issn.1008-0384.2015.11.001

Citation:

WAN Chun-he, PAN Yi-zhe, FU Qiu-ling, CHEN Zhen, CHEN Cui-teng, FU Guang-hua, CHEN Hong-mei, CHENG Long-fei, SHI Shao-hua, HUANG Yu. NS1-ELISA for Antibody Detection on Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1027-1031. doi: 10.19303/j.issn.1008-0384.2015.11.001

Citation:

WAN Chun-he, PAN Yi-zhe, FU Qiu-ling, CHEN Zhen, CHEN Cui-teng, FU Guang-hua, CHEN Hong-mei, CHENG Long-fei, SHI Shao-hua, HUANG Yu. NS1-ELISA for Antibody Detection on Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1027-1031. doi: 10.19303/j.issn.1008-0384.2015.11.001

PDF( 1230 KB)

NS1-ELISA for Antibody Detection on Avian Tembusu Virus

doi: 10.19303/j.issn.1008-0384.2015.11.001

1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences/Fujian Animal Disease Control Technology Development Center, Fuzhou, Fujian 350013, China;
2. Zhejiang Huzhou Center for Animal Disease Control and Prevention, Huzhou, Zhejiang 313000, China

Received Date: 2015-08-31
Publish Date: 2015-11-18

Abstract

Abstract

Nonstructural protein 1(NS1)from the avian Tembusu virus were amplified and expressed in E.colito establish ELISA procedures for the detection of antibodies in the virus.Biological activity of the expressed protein was analyzed by using SDS-PAGE and western blot.The results indicated that the recombinant NS1 with a molecular weight of 60 kD was expressed in high level with good biological activity.It was subsequently used to establish an indirect ELISA for the detection of antibodies in the virus.The optimized procedures included the uses of 2.5μg·mL^-1 recombination protein,1∶100serum dilution,and the goat anti-duck IgG conjugate(1∶3000).Although the NS1-ELISA showed desirable specificity and repeatability,it failed to effectively differentiate the inactivated Tembusu vaccine serumfrom the naturally infected serum.Thus,this method could not be applied as a diagnosis tool for Tembusu virus.
- avian tembusu virus,
- nonstructural protein 1,
- expression,
- indirect ELISA

FullText(HTML)

References(19)

References

[1]	万春和,施少华,程龙飞,等.一种引起种(蛋)鸭产蛋骤降新病毒的分离与初步鉴定[J].福建农业学报,2010,25(6):663-666.
[2]	曹贞贞,张存,黄瑜,等.鸭出血性卵巢炎的初步研究[J].中国兽医杂志,2010,46(12):3-6.
[3]	SU J,LI S,HU X,et al.Duck egg-drop syndrome caused by BYD virus,a new Tembusu-realated Flavivirus[J].PLoS one,2011,6(3):e18106.
[4]	CAO Z,ZHANG C,LIU Y,et al.Tembusu virus in ducks,China[J].Emerg Infect Dis,2011,17(10):1873-1875.
[5]	YAN P,ZHAO Y,ZHANG X,et al.An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China[J].Virology,2011,417(1):1-8.
[6]	傅光华,黄瑜,施少华,等.鸡黄病毒的分离与初步鉴定[J].福建畜牧兽医,2011,33(3):1-2.
[7]	刘友生,彭春香,傅光华,等.2010-2011年中国部分地区禽坦布苏病毒感染调查及分子变异分析[J].中国动物传染病学报,2012,20(1):47-53.
[8]	LIU P,LU H,LI S,et al.Duck egg drop syndrome virus:an emerging Tembusu-related flavivirus in China[J].Sci China Life Sci,2013,56(8):701-710.
[9]	TANG Y,GAO X,DIAO Y,et al.Tembusu virus in human,China[J].Transbound Emerg Dis,2013,60(3):193-196.
[10]	LIU M,LIU C,LI G,et al.Complete genomic sequence of duck flavivirus from china[J].J Virol,2012,86(6):3398-3399.
[11]	YUN T,ZHANG D,MA X,et al.Complete genome sequence of a novel flavivirus,duck tembusu virus,isolated from ducks and geese in china[J].J Virol,2012,86(6):3406-3407.
[12]	WAN C,HUANG Y,FU G,et al.Complete genome sequence of avian tembusu-related virus strain WR isolated from White Kaiya ducks in Fujian,China[J].J Virol,2012,86(19):10912.
[13]	CHAO D,LIU Y,SHEN W,et al.Comparison of E and NS1antigens capture ELISA to detect dengue viral antigens from mosquitoes[J].J Vector Borne Dis,2015,52(2):134-141.
[14]	陈月,任瑞文,刘建伟.登革病毒非结构蛋白1结构及功能研究进展[J].病毒学报,2014,(6):88-93.
[15]	傅强,田疆,方丹云,等.登革病毒NS1蛋白的原核表达及其在登革热快速诊断中的应用[J].中山大学学报:自然科学版,2012,33(3):316-321.
[16]	RATHAKRISHNAN A,SEKARAN S.New development in the diagnosis of dengue infections[J].Expert Opin Med Diagn,2013,7(1):99-112.
[17]	GELANEW T,POOLE-SMITH B,HUNSPERGER E.Development and characterization of mouse monoclonal antibodies against monomeric dengue virus non-structural glycoprotein 1(NS1)[J].J Virol Methods,2015,222:214-223.
[18]	傅秋玲,陈珍,施少华,等.鸭坦布苏病毒E蛋白包被抗原间接ELISA方法的建立[J].福建农业学报,2015,30(1):1-5.
[19]	万春和,傅秋玲,陈珍,等.乳胶凝集试验方法在检测鸭坦布苏病毒抗体中的应用[J].福建农业学报,2013,28(12):1200-1203.