Tissue Culture and Rapid Propagation of Big Chili Phalaenopsis

WU Ai-long; WU Jian-yang; ZHUO Hai-rong

doi:10.19303/j.issn.1008-0384.2015.11.010

Volume 30 Issue 11

Nov. 2015

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WU Ai-long, WU Jian-yang, ZHUO Hai-rong. Tissue Culture and Rapid Propagation of Big Chili Phalaenopsis[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1075-1081. doi: 10.19303/j.issn.1008-0384.2015.11.010

Citation:

WU Ai-long, WU Jian-yang, ZHUO Hai-rong. Tissue Culture and Rapid Propagation of Big Chili Phalaenopsis[J]. Fujian Journal of Agricultural Sciences, 2015, 30(11): 1075-1081. doi: 10.19303/j.issn.1008-0384.2015.11.010

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Tissue Culture and Rapid Propagation of Big Chili Phalaenopsis

doi: 10.19303/j.issn.1008-0384.2015.11.010

1. College of Basic Education, Zhanjiang Normal College, Zhanjiang, Guangdong 524037, China;
2. Huazhou High School, Maoming, Guangdong 525100, China

Received Date: 2015-05-08
Publish Date: 2015-11-18

Abstract

Abstract

The young,tender flower stalks of the Big Chili Phalaenopsis were used for this study on the tissue culture and rapid propagation of the orchid.Using an orthogonal design experiment,the effects of the basic culture medium as well as hormone application and concentration on the pedicelinduction,proliferation and differentiation of the adventitious buds and the seedling development and rooting of the plant were investigated.The results showed that(a)the best medium for inducing the adventitious buds was Hyponex No.1+NAA(0.3mg·L^-1)+6-BA(5mg·L^-1);(b)the best medium for the proliferation and differentiation of the adventitious buds was Hyponex No.1+NAA(0.2mg·L^-1)+6-BA(6mg·L^-1),which resulted in a multiplication factor up to 5.53times;(c)the best medium forseedling development and rooting was 1/2MS+NAA(0.2mg·L^-1),which yielded a rooting rate of 96%,and coconut milk,apple juice,potato juice as parts of the composite additives were found to be advantageous for rooting;and(d)the survival rate of the transplanted tissue culture was greater than 91.5%.
- Phalaenopsis,
- orthogonal experiment,
- tissue culture

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References(16)

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