Effect of Organic Solvents on Activity of N-Acetyl-β-D-glucosaminidase from Integument of Fenneropenaeus chinensis

LIN Juan-juan; ZHU Qiu-xiang; LIN Jian-cheng

doi:10.19303/j.issn.1008-0384.2015.12.003

Volume 30 Issue 12

Dec. 2015

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Article Navigation > Fujian Journal of Agricultural Sciences > 2015 > 30(12): 1143-1148

LIN Juan-juan, ZHU Qiu-xiang, LIN Jian-cheng. Effect of Organic Solvents on Activity of N-Acetyl-β-D-glucosaminidase from Integument of Fenneropenaeus chinensis[J]. Fujian Journal of Agricultural Sciences, 2015, 30(12): 1143-1148. doi: 10.19303/j.issn.1008-0384.2015.12.003

Citation:

LIN Juan-juan, ZHU Qiu-xiang, LIN Jian-cheng. Effect of Organic Solvents on Activity of N-Acetyl-β-D-glucosaminidase from Integument of Fenneropenaeus chinensis[J]. Fujian Journal of Agricultural Sciences, 2015, 30(12): 1143-1148. doi: 10.19303/j.issn.1008-0384.2015.12.003

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Effect of Organic Solvents on Activity of N-Acetyl-β-D-glucosaminidase from Integument of Fenneropenaeus chinensis

doi: 10.19303/j.issn.1008-0384.2015.12.003

College of Environmental and Biological Engineering, Putian University, Putian, Fujian 351100, China

Received Date: 2015-09-04
Publish Date: 2015-12-18

Abstract

Abstract

Effects of 10 organic solvents on the activity of N-Acetyl-β-D-glucosaminidase(NAGase)from the integument of Fenneropenaeus chinensis were investigated.The results indicated that the enzyme was activated in methanol,ethanol,propanol,glycol,glycerol,dimethyl sulfoxide,dioxane,and acetone in low concentrations,but inhibited in high concentrations.It was also activated in 0%to 55% butanol.The enzymatic activity was apparently formaldehyde-concentration dependent.A further study on formaldehyde and acetone showed that the inhibition by either solvent was reversible in high concentrations,and the activation,in acetone in low concentrations.The results demonstrated the varying effects of the organic solvents in different concentrations on the activity of NAGase.
- Fenneropenaeus chinensis,
- organic solvent,
- N-Acetyl-β-D-glucosaminidase,
- enzyme activity,
- integument

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References(20)

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