Screening and Properties of Probiotic Bacillus

LI An-qi; LAN Jiang-lin; LIU Bo; HUANG Su-fang

doi:10.19303/j.issn.1008-0384.2015.12.010

Volume 30 Issue 12

Dec. 2015

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LI An-qi, LAN Jiang-lin, LIU Bo, HUANG Su-fang. Screening and Properties of Probiotic Bacillus[J]. Fujian Journal of Agricultural Sciences, 2015, 30(12): 1184-1192. doi: 10.19303/j.issn.1008-0384.2015.12.010

Citation:

LI An-qi, LAN Jiang-lin, LIU Bo, HUANG Su-fang. Screening and Properties of Probiotic Bacillus[J]. Fujian Journal of Agricultural Sciences, 2015, 30(12): 1184-1192. doi: 10.19303/j.issn.1008-0384.2015.12.010

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Screening and Properties of Probiotic Bacillus

doi: 10.19303/j.issn.1008-0384.2015.12.010

1. College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350003, China;
2. Agricultural Bioresource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2015-11-07
Publish Date: 2015-12-18

Abstract

Abstract

Screened from a collection of bacillus bacteria,the antibacterial and protease-producing Brevibacillus formosus FJAT-10011 was selected.Characteristics of the protease and antibacterial properties of its fermentation supernatant were studied.The peak activity of the protease was found at pH 6.0and 40℃.However,the enzyme remained stable below40℃ and between pH 5.0and 9.0.The anti-enterobacterial component of the supernatant exhibited a high temperature stability–its activity decreased merely 20%at 80℃in 30 minutes.Furthermore,the stability was the highest in the range of pH 6.0to 8.0,and not affected by proteinase K,pepsin or trypsin.In addition,FJAT-10011 could also inhibit Fusarium oxysporum,similar to the effect by the antibiotic,hygromycin,at 1mg·mL-1.
- Brevibacillus formosus,
- enzyme characteristics,
- antibacterial properties

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References(30)

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