Hot-water Extraction of Polysaccharides from <em>Chlorella pyrenoidosa</em> with Cellulase

CHEN Yi-xuan; LIU Xiao-yan; WU Lin-xiu; LUO Hong-jian; LIU Bin

doi:10.19303/j.issn.1008-0384.2016.05.013

Volume 31 Issue 5

Jun. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(5): 508-514

CHEN Yi-xuan, LIU Xiao-yan, WU Lin-xiu, LUO Hong-jian, LIU Bin. Hot-water Extraction of Polysaccharides from Chlorella pyrenoidosa with Cellulase[J]. Fujian Journal of Agricultural Sciences, 2016, 31(5): 508-514. doi: 10.19303/j.issn.1008-0384.2016.05.013

Citation:

CHEN Yi-xuan, LIU Xiao-yan, WU Lin-xiu, LUO Hong-jian, LIU Bin. Hot-water Extraction of Polysaccharides from Chlorella pyrenoidosa with Cellulase[J]. Fujian Journal of Agricultural Sciences, 2016, 31(5): 508-514. doi: 10.19303/j.issn.1008-0384.2016.05.013

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Hot-water Extraction of Polysaccharides from Chlorella pyrenoidosa with Cellulase

doi: 10.19303/j.issn.1008-0384.2016.05.013

1.
Fujian Agriculture and Forestry University, College of Food Science, Fuzhou, Fujian 350002, China

Received Date: 2016-02-27
Rev Recd Date: 2016-03-18
Publish Date: 2016-05-28

Abstract

Abstract

Cellulase was added to the hot water extraction of polysaccharides from Chlorella pyrenoidosa in an attempt to facilitate the process. The single factor test and the response surface methodology were used to optimize the conditions. It was found that whenthe substrate:liquid ratio of 1:30(gmL-1) with 2%(w/w) of cellulase addition was extracted at 93℃ for 2 h, an expected 6.13% yield on polysaccharidescould be obtained (the actual yield was 5.870.24%). Subsequently, the polysaccharides obtained was tested for its scavenging activities on DPPH,O2-, and hydroxyl free radicals.The results confirmed itsantioxidant abilities agains O2- and hydroxyl free radicals.
- Chlorella pyrenoidosa,
- polysaccharide,
- cellulase,
- response surface,
- antioxidant activity

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References(3)

References

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