HPLC Determination of Omethoate Content in Soil

LI Ruo-yun; ZHANG Jiao

doi:10.19303/j.issn.1008-0384.2016.08.017

Volume 31 Issue 8

Oct. 2016

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(8): 881-885

LI Ruo-yun, ZHANG Jiao. HPLC Determination of Omethoate Content in Soil[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 881-885. doi: 10.19303/j.issn.1008-0384.2016.08.017

Citation:

LI Ruo-yun, ZHANG Jiao. HPLC Determination of Omethoate Content in Soil[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 881-885. doi: 10.19303/j.issn.1008-0384.2016.08.017

LI Ruo-yun, ZHANG Jiao. HPLC Determination of Omethoate Content in Soil[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 881-885. doi: 10.19303/j.issn.1008-0384.2016.08.017

Citation:

LI Ruo-yun, ZHANG Jiao. HPLC Determination of Omethoate Content in Soil[J]. Fujian Journal of Agricultural Sciences, 2016, 31(8): 881-885. doi: 10.19303/j.issn.1008-0384.2016.08.017

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HPLC Determination of Omethoate Content in Soil

doi: 10.19303/j.issn.1008-0384.2016.08.017

LI Ruo-yun^{1, 2
,},
ZHANG Jiao^{1, 2
,
,}

1.
Qingdao Engineering Research Center for Rural Environment College of Resource and Environment, Qingdao, Shandong 266109, China
2.
Qingdao Agricultural University, Qingdao, Shandong 266109, China

Received Date: 2016-04-07
Rev Recd Date: 2016-05-20
Publish Date: 2016-08-01

Abstract

Abstract

Testing conditions for the omethoate determination byHPLC were optimized to be: Inertsil QDS-SP (4.6*250 μm) at 25℃, an injection of 20μL methanol/water (V/V=10/90) as mobile phase ata flow rate of 1.0 mL·min^-1, and the wave length of 210 nm for ultraviolet detection. A high recovery rate was achieved by using anextraction column with acetone/acetonitrile (V/V=1/3) as the eluting agent. A linear calibration curve for the omethoate concentrations in the range of 0.1-10μg·mL^-1(R²=0.9992) with a minimal detection limit of 0.1 μg·mL^-1 was established. Acetone and ethyl acetate were used as the solvents in the oscillation extraction. The recovery rate of omethoate in the soil samples ranged from 70% to 120% with a relative deviation of 2.08%-8.08%, which was within the requirements for pesticide residue analysis. It appeared that the newly developed HPLC methodology was appropriate for detecting omethoate residues in soil.
- HPLC,
- omethoate,
- soil,
- organophosphorus pesticides

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References(22)

References

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