Protocorm Induction/Differentiation and Plantlet Regeneration of Stems from <i>Dendrobium officinale</i> Kimura et Migo

LIN Jiang-bo; WANG Wei-ying; LI Hai-ming; WU Shui-jin; ZOU Hui; LI Yue-sen; DAI Yi-min

doi:10.19303/j.issn.1008-0384.2016.10.014

Volume 31 Issue 10

Dec. 2016

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(10): 1075-1079

LIN Jiang-bo, WANG Wei-ying, LI Hai-ming, WU Shui-jin, ZOU Hui, LI Yue-sen, DAI Yi-min. Protocorm Induction/Differentiation and Plantlet Regeneration of Stems from Dendrobium officinale Kimura et Migo[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1075-1079. doi: 10.19303/j.issn.1008-0384.2016.10.014

Citation:

LIN Jiang-bo, WANG Wei-ying, LI Hai-ming, WU Shui-jin, ZOU Hui, LI Yue-sen, DAI Yi-min. Protocorm Induction/Differentiation and Plantlet Regeneration of Stems from Dendrobium officinale Kimura et Migo[J]. Fujian Journal of Agricultural Sciences, 2016, 31(10): 1075-1079. doi: 10.19303/j.issn.1008-0384.2016.10.014

Citation:

PDF( 922 KB)

Protocorm Induction/Differentiation and Plantlet Regeneration of Stems from Dendrobium officinale Kimura et Migo

doi: 10.19303/j.issn.1008-0384.2016.10.014

Subtropical Agriculture Research Institute, Fujian Academy of Agricultural Sciences, Zhangzhou, Fujian 363005, China

Received Date: 2016-04-11
Rev Recd Date: 2016-06-28
Publish Date: 2016-10-01

Abstract

Abstract

To establish an effective and rapid propagation method for Dendrobium officinale, sterile stem cuttings from the plant were used as the explants for the study. Effects of cultivation media, growth regulators, sucrose concentrations, and additives on the protocorm induction, proliferation and differentiation, as well as the rooting of the plantlets were investigated in an orthogonal experiment. The results showed that the optimal medium for the protocorm induction consisted of 1/2MS+6-BA4 mg·L^-1+NAA0.1 mg·L^-1; that for the proliferation, MS+30 g·L^-1; that for the differentiation, MS+10 g·L^-1sucrose+10% potato slurry; and, that for the rooting, 1/2MS+NAA1 mg·L^-1+10% potato slurry+10% banana slurry. The method appeared to enable a successful regeneration of D. officinale plantlets through tissue culture propagation.
- Dendrobium officinale,
- protocorm,
- orthogonal experiment,
- plantlet regeneration

FullText(HTML)

References(16)

References

[1]	中华人民共和国国家药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社, 2010:265-266.
[2]	高建平, 金若敏, 吴耀平, 等.铁皮石斛原球茎与原药材免疫调节作用的比较研究[J].中药材, 2002, 25(7):487-489. http://www.cnki.com.cn/Article/CJFDTOTAL-ZYCA200207015.htm
[3]	张红玉, 戴关海, 马翠, 等.铁皮石斛多糖对S (180)肉瘤小鼠免疫功能的影响[J].浙江中医杂志, 2009, 44(5):380-381. http://www.cnki.com.cn/Article/CJFDTOTAL-ZJZZ200905055.htm
[4]	吴昊姝, 徐建华, 陈立钻, 等.铁皮石斛降血糖作用及其机制的研究[J].中国中药杂志, 2004, 29(20):161-164. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY200402020.htm
[5]	何铁光, 杨丽涛, 李杨瑞, 等.铁皮石斛原球茎多糖粗品与纯品的体外抗氧活性研究[J].中成药, 2007, 29(9):1265-1269. http://www.cnki.com.cn/Article/CJFDTOTAL-ZCYA200709005.htm
[6]	林江波, 戴艺民, 邹晖, 等.福建铁皮石斛人工繁育技术研究[J].福建农业学报, 2010, 25(5)406-410.
[7]	戚华沙, 潘梅, 黄赛, 等.铁皮石斛茎段组织培养技术研究[J].安徽农学通报, 2013, 19(13):32-34. http://www.cnki.com.cn/Article/CJFDTOTAL-AHNB201313016.htm
[8]	蒋波, 杨存亮, 黄捷, 等.铁皮石斛原球茎生长分化及生根壮苗研究[J].玉林师范学院学报:自然科学版, 2005, 26(3):66-69. http://www.cnki.com.cn/Article/CJFDTOTAL-YLSZ200503019.htm
[9]	林丛发, 钟爱清, 林云斌, 等.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报, 2007, 19(1):84-86. http://www.cnki.com.cn/Article/CJFDTOTAL-JXNY200701034.htm
[10]	唐启义, 冯光明.实用统计分析及其DPS数据处理系统[M].北京:农业出版社, 2002, 94-99.
[11]	赵兴兵, 吴维佳, 庞璐, 等.珍稀濒危药材铁皮石斛组培快繁关键技术研究[J].湖南中医药大学学报, 2012, 32(3):27-30. http://www.cnki.com.cn/Article/CJFDTOTAL-HNZX201203009.htm
[12]	李丹, 王馨彩, 尹明华.黑节铁皮石斛原球茎诱导、再分化以及腋芽萌发和生根的初步研究[J].上饶师范学院学报, 2010, 30(6):74-78. http://www.cnki.com.cn/Article/CJFDTOTAL-SRSX201006017.htm
[13]	邵世光, 侯北伟, 周琪, 等.基于正交实验法的铁皮石斛原球茎分化和生根条件研究[J].南京师范大学学报:自然科学版, 2009, 32(4):98-102. http://www.cnki.com.cn/Article/CJFDTOTAL-NJSF200904021.htm
[14]	杨柳平, 刘畅庆, 赵仁发, 等.铁皮石斛种子诱导原球茎组培快繁体系的研究[J].广东农业科学.2012(7):54-57. http://www.cnki.com.cn/Article/CJFDTOTAL-GDNY201207024.htm
[15]	李海燕, 林丽婷, 谢燕婷, 等.铁皮石斛组培苗生根壮苗培养基的优化筛选研究[J].佛山科学技术学院学报:自然科学版, 2015, 33(2):22-27. http://www.cnki.com.cn/Article/CJFDTOTAL-FSDX201502006.htm
[16]	罗焕明, 邓瑞云, 刘晓津, 等.不同添加物及浓度对铁皮石斛组织培养的影响[J].广东农业科学, 2014, (7):30-32. http://www.cnki.com.cn/Article/CJFDTOTAL-GDNY201407009.htm