Oleic-acid-producing <i>Bacillus</i>-like Bacteria Screened by Means of Chemomics

XIAO Tong-fen; WANG Jie-ping; LIU Bo; CHEN Zheng; LIU Guo-hong; CHEN Qian-qian; CHE Jian-mei; GE Ci-bin

doi:10.19303/j.issn.1008-0384.2016.12.016

Volume 31 Issue 12

Feb. 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2016 > 31(12): 1345-1352

XIAO Tong-fen, WANG Jie-ping, LIU Bo, CHEN Zheng, LIU Guo-hong, CHEN Qian-qian, CHE Jian-mei, GE Ci-bin. Oleic-acid-producing Bacillus-like Bacteria Screened by Means of Chemomics[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1345-1352. doi: 10.19303/j.issn.1008-0384.2016.12.016

Citation:

XIAO Tong-fen, WANG Jie-ping, LIU Bo, CHEN Zheng, LIU Guo-hong, CHEN Qian-qian, CHE Jian-mei, GE Ci-bin. Oleic-acid-producing Bacillus-like Bacteria Screened by Means of Chemomics[J]. Fujian Journal of Agricultural Sciences, 2016, 31(12): 1345-1352. doi: 10.19303/j.issn.1008-0384.2016.12.016

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Oleic-acid-producing Bacillus-like Bacteria Screened by Means of Chemomics

doi: 10.19303/j.issn.1008-0384.2016.12.016

1.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian 350108, China
2.
Institute of Agro-biological Resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

Received Date: 2016-05-10
Rev Recd Date: 2016-08-05
Publish Date: 2016-12-28

Abstract

Abstract

Extracellular metabolites in the supernatants 52 Bacillus-like strains were analyzed by HPLC along with the quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS). Subsequently, the molecular feature extraction (MFE) was performed on the data using the software MassHunter followed by submitting the information for comparison against the mass spectrometry database, Metlin. Free oleic acid was thereby detected in the cultural supernatant of Bacillus plakortidis, FJAT-14858. Its matching score with oleic acid was 95.61, accurate mass was 284.2693, and relative content in the metabolites collected was up to 1.62%. To date, B. plakortidis FJAT-14858 was the first Bacillus-like bacterium known to produce free oleic acid with no cytotoxicity to the bacteria per se.
- Bacillus plakortidis FJAT-14858,
- free oleic acid,
- chemomics,
- HPLC-quadrupole time-of-flight MS

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References(36)

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