Molecular Identification of Juvenile Eels by DNA Fingerprints

LIN Neng-feng; CHEN Qiang; YANG Jin-xian; XU Bin-fu; ZENG Hong

doi:10.19303/j.issn.1008-0384.2017.03.006

Volume 32 Issue 3

May 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(3): 258-262

LIN Neng-feng, CHEN Qiang, YANG Jin-xian, XU Bin-fu, ZENG Hong. Molecular Identification of Juvenile Eels by DNA Fingerprints[J]. Fujian Journal of Agricultural Sciences, 2017, 32(3): 258-262. doi: 10.19303/j.issn.1008-0384.2017.03.006

Citation:

LIN Neng-feng, CHEN Qiang, YANG Jin-xian, XU Bin-fu, ZENG Hong. Molecular Identification of Juvenile Eels by DNA Fingerprints[J]. Fujian Journal of Agricultural Sciences, 2017, 32(3): 258-262. doi: 10.19303/j.issn.1008-0384.2017.03.006

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Molecular Identification of Juvenile Eels by DNA Fingerprints

doi: 10.19303/j.issn.1008-0384.2017.03.006

1.
Institute of Biotechnology, Fujian Academy of Agriculture Sciences, Fuzhou, Fujian 350003, China
2.
College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China

Received Date: 2016-08-22
Rev Recd Date: 2017-01-10
Publish Date: 2017-03-01

Abstract

Abstract

Accurate species identification is crucial for eel aquaculture, especially when increasing taxa of the fish were raised in the eel farms in China. This study applied a pair of specific primers from the conservative fragment of mtDNA COI gene in Anguilla to amplify 109 DNA samples extracted from 21 batches of eel juveniles for taxon identification. It was found that a specific 652bp fragment could be amplified from all of the samples. The BOLD Identification System (Barcoding of Life Data Systems: www.boldsystems.org) was used for sequence similarity search on the COI genes. Seven species, including A. japonica, A. rostrata, A.anguilla, A.marmorata, A.mossambica, A.luzonensis and A.bicolor pacifica, were identified. The cluster analysis showed 7 main clades with high rate of support at the nodes. It suggested that the COI identification method could be satisfactorily employed for eel juveniles identification. Incidentally, during the course of this study a former mistaken identification on a batch of juvenile fish was discovered. Therefore, the DNA barcode-based identification method was highly recommended for implementation to benefit the commercial trade and eel aquaculture.
- eel,
- juveniles,
- COI,
- molecular identification

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References(15)

References

[1]	HEBERT P D N, RATNASINGHAM S, DEWAARD J R. Barcoding animal life:cytochrome c oxidase subunit 1 divergences among closely related species[J]. Proceedings:Biological Sciences, 2003, 270:S96-S99. doi: 10.1098/rsbl.2003.0025
[2]	WARD R D, HANNER R, HEBERT P D N. The campaign to DNA barcode all fishes, FISH-BOL[J].Journal of Fish Biology, 2009, 74(2):329-356. doi: 10.1111/jfb.2009.74.issue-2
[3]	林能锋, 苏永全, 丁少雄, 等.大黄鱼微卫星标记引物在石首鱼科几个近缘种中的通用性研究[J].中国水产科学, 2008, 15(2):237-243. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSCK200802007.htm
[4]	UNTERGASSER A, CUTCUTACHE I, KORESSAAR T, et al.Primer3-new capabilities and interfaces[J]. Nucleic Acids Research, 2012, 40(15):e115. doi: 10.1093/nar/gks596
[5]	KORESSAAR T, REMM M.Enhancements and modifications of primer design program Primer3[J].Bioinformatics, 2007, 23(10):1289-1291. doi: 10.1093/bioinformatics/btm091
[6]	RATNASINGHAM S and HEBERT P D N. BOLD:The barcode of life data system (www.barcodinglife.org)[J]. Molecular Ecology Note, 2007, 7:355-364. doi: 10.1111/j.1471-8286.2007.01678.x
[7]	KUMAR S, STECHER G and TAMURA K. MEGA7:Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution, 2016, 33(7):1870-1874. doi: 10.1093/molbev/msw054
[8]	BASTROP R, STREHLOW B, JURSS K, STURMBAUER C. A new molecular phylogenetic hypothesis for the evolution of freshwater eels[J]. Molecular Phylogenetics and Evolution, 2000, 14(2):250-258. doi: 10.1006/mpev.1999.0694
[9]	AOYAMA J, NISHIDA M, TSUKAMOTO K. Molecular phylogeny and evolution of the freshwater eel, genus Anguilla[J]. Molecular Phylogenetics and Evolution, 2001, 20(3):450-459. doi: 10.1006/mpev.2001.0959
[10]	罗土炎, 饶小珍, 林岗, 等. 4种鳗鲡同工酶的比较研究[J].福建农业学报, 2009, 24(5):421-428. http://www.fjnyxb.cn/CN/abstract/abstract385.shtml
[11]	杨弘, 王希道, 吴婷婷, 等.用RAPD技术研究3种鳗鱼的种质鉴定[J].中国水产科学, 2002, 9(3):269-272. http://www.cnki.com.cn/Article/CJFDTOTAL-ZSCK200203018.htm
[12]	张新艳, 樊海平, 范斯敏, 等.应用RAPD技术对6种玻璃鳗的种质鉴定和遗传多样性分析[J].江西农业大学学报, 2010, 32(4):683-688. http://www.cnki.com.cn/Article/CJFDTOTAL-JXND201004013.htm
[13]	梁俊, 李道季, 卢莉琼.日本鳗鲡 (Anguilla japonica) 和欧洲鳗鲡 (A.anguilla) 的微卫星差异[J].海洋与湖沼, 2003, 34(4):414-421. doi: 10.11693/hyhz200304008008
[14]	HEBERT P D N, CYWINSKA A, BALL S L, et al. Biological identifications through DNA barcodes[J]. Proc.R. Soc. Lond.B, 2003, 70:313-321.
[15]	吴宁, 黎中宝, 林小云, 等. 6种鳗鲡 (Anguilla) 线粒体DNA COI序列的比较研究[J].海洋与湖沼, 2010, 41(6):930-934. doi: 10.11693/hyhz201006020020