Discovery and Mechanisms of DNA Interference

RUI Xiang-yun; CHEN Jin-zhi; HUANG Peng-rong; YANG Guang

doi:10.19303/j.issn.1008-0384.2017.04.019

Volume 32 Issue 4

May 2017

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Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(4): 456-460

RUI Xiang-yun, CHEN Jin-zhi, HUANG Peng-rong, YANG Guang. Discovery and Mechanisms of DNA Interference[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 456-460. doi: 10.19303/j.issn.1008-0384.2017.04.019

Citation:

RUI Xiang-yun, CHEN Jin-zhi, HUANG Peng-rong, YANG Guang. Discovery and Mechanisms of DNA Interference[J]. Fujian Journal of Agricultural Sciences, 2017, 32(4): 456-460. doi: 10.19303/j.issn.1008-0384.2017.04.019

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Discovery and Mechanisms of DNA Interference

doi: 10.19303/j.issn.1008-0384.2017.04.019

RUI Xiang-yun^{1, 2, 3
,},
CHEN Jin-zhi^{1, 2, 3},
HUANG Peng-rong^{1, 2, 3},
YANG Guang^{1, 2, 3
,
,}

1.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou, Fujian 350002, China
2.
Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops, Ministry of Agriculture, China, Fuzhou, Fujian 350002, China
3.
Fujian-Taiwan Joint Innovation Centre for Ecological Control of Crop Pests, Fuzhou, Fujian 350002, China

Received Date: 2017-01-08
Rev Recd Date: 2017-02-12
Publish Date: 2017-04-28

Abstract

Abstract

DNA fragment introduced into cells can specifically inhibit the expression of homologous gene, which is identified as DNA interference (DNAi). DNAi was discovered with the events of post-transcriptional gene silencing in tobacco, and subsequently explored in other plants and animals. DNAi was present in prokaryotes and in vitro DNAi was also achieved by Ago proteins from prokaryotes. The mechanism of DNAi in prokaryotes is mostly that Ago proteins are complexed with small DNA guide to cleave DNA or RNA. Post-transcriptional gene silencing, genome methylation and transcriptional repression of promoter binding might be also covered in the mechanisms of DNAi in eukaryotes. In addition, the further research and application of DNAi were discussed and prospected.
- DNA interference,
- gene silencing,
- ago protein

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References(30)

References

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