Identification and Genetic Diversity of <i>Polygonatum cyrtonema</i> Hua and <i>P.filipes</i> Merr. Based on ISSR Marker

XU Hui-long; WANG Ying-jun; CHEN Ming; FAN Xiao-fang; ZHUO Fei-ying; WEI Yi-cong; FAN Shi-ming

doi:10.19303/j.issn.1008-0384.2017.06.009

Volume 32 Issue 6

Aug. 2017

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2017 > 32(6): 619-624

XU Hui-long, WANG Ying-jun, CHEN Ming, FAN Xiao-fang, ZHUO Fei-ying, WEI Yi-cong, FAN Shi-ming. Identification and Genetic Diversity of Polygonatum cyrtonema Hua and P.filipes Merr. Based on ISSR Marker[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 619-624. doi: 10.19303/j.issn.1008-0384.2017.06.009

Citation:

XU Hui-long, WANG Ying-jun, CHEN Ming, FAN Xiao-fang, ZHUO Fei-ying, WEI Yi-cong, FAN Shi-ming. Identification and Genetic Diversity of Polygonatum cyrtonema Hua and P.filipes Merr. Based on ISSR Marker[J]. Fujian Journal of Agricultural Sciences, 2017, 32(6): 619-624. doi: 10.19303/j.issn.1008-0384.2017.06.009

Citation:

PDF( 1215 KB)

Identification and Genetic Diversity of Polygonatum cyrtonema Hua and P.filipes Merr. Based on ISSR Marker

doi: 10.19303/j.issn.1008-0384.2017.06.009

1.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
2.
Fujian Collaborative Innovation Center for Rehabilitation Technology, Fuzhou, Fujian 350122, China

Received Date: 2017-01-11
Rev Recd Date: 2017-05-11
Publish Date: 2017-06-28

Abstract

Abstract

Identification and genetic diversity analysis based on ISSR marker for 19 germplasms of Polygonatum were conducted. The results were as follows. (1) Based upon the morphological characteristics of the plants, germplasms No. 1, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, and 19 were determined to be P. cyrtonema Hua; and No. 2, 3, 4, 5, 14, and 17, P. filipes Merr. (2) From the 100 primers isolated, 11 were amplified to render 170 distinctive and polymorphic bands with sizes ranging from 250 bp to 200 0 bp. (3) Using Popgen 32 software, an abundant genetic diversity was found to exist among these germplasms with similarity coefficients ranging from 0.604 9 to 0.824 4, and genetic distances from 0.193 1 to 0.502 7; and, No. 6 and 7 were closely related, while the No. 10 and 17 differed the most. (4) The UPGMA cluster analysis classified the germplasms into 2 groups and 4 subgroups with all P. cyrtonema Hua in Group Ⅰ and all P. filipes Merr. in Group Ⅱ. And, (5) as analyzed by Popgen 32, the genetic diversity of the 4 subclasses showed an effective number of alleles (Ne) to be 1.484 5, Nei's genetic diversity index (h), 0.292 3, Shannon diversity index (Ⅰ), 0.423 9, and total gene diversity (Ht), 0.413 2. It appeared that the current method based upon ISSR marker could be used to identify the individual species as well as show the genetic diversity and relationship among the species in the genus providing valuable molecular information for the introduction, acclimation, protection and utilization of the Polygonatum resources in the wild.
- Polygonatum cyrtonema Hua,
- Polygonatum filipes Merr,
- germplasm resource,
- ISSR identification,
- genetic diversity

FullText(HTML)

References(17)

References

[1]	国家药典委员会.中华人民共和国药典:一部[M].2015年版.北京:中国医药科技出版社, 2015:306-307.
[2]	王婷, 苗明三.黄精的化学、药理及临床应用特点分析[J].河南中医学院学报, 2015, 30(5):714-715. http://www.cnki.com.cn/Article/CJFDTOTAL-HNZK201505037.htm
[3]	中国科学院植物研究所. 中国在线植物志[DB/OL][2016-12-01]. http://frps.eflora.cn/frps/Polygonatum%20cyrtonema.
[4]	福建省中医药研究院.福建药物志(第二卷修订本)[M].福州:福建科学技术出版社, 1994:462.
[5]	周晔, 王润玲, 唐铖, 等.ISSR法鉴定中药黄精与卷叶黄精[J].天津医科大学学报, 2006, 12(2):178-181. http://www.cnki.com.cn/Article/CJFDTOTAL-TJYK200602006.htm
[6]	INNIS A F, FORSETH I N, WHIGHAM D F, et al. Genetic diversity in the invasive Rubus phoenicolasius as compared to the native Rubus argutus using inter-simple sequence repeat (ISSR)markers[J]. Biological Invasions, 2011, 13(8): 1735-1738. doi: 10.1007/s10530-011-0012-0
[7]	ZHAO G H, LI J, ZOU F C, et al. ISSR, an effective molecular approach for studying genetic variability among Schistosoma japonicum isolates from different provinces in main land China[J]. Infect Genet Evol, 2009, 9(5): 903-907. doi: 10.1016/j.meegid.2009.06.006
[8]	TAO H. Assessing genetic diversity of perennial ryegrass (Lolium perenne L.) from four continents by inter-simple sequence repeat (ISSR) markers[J]. African Journal of Biotechnology, 2011, 10(83): 19365-19374. https://www.researchgate.net/publication/272690084_Assessing_genetic_diversity_of_perennial_ryegrass_Lolium_perenne_L_from_four_continents_by_inter-simple_sequence_repeat_ISSR_markers
[9]	周晔, 王润玲, 唐铖, 等.RAPD标记法鉴定中药黄精及长梗黄精的研究[J].时珍国医国药, 2007, 18(9): 2149-2150. http://www.cnki.com.cn/Article/CJFDTOTAL-SZGY200709047.htm
[10]	杨培, 周红, 辛天怡, 等.黄精属药用植物DNA条形码鉴定研究[J].世界中医药, 2015, 10(8):1173-1176. http://www.cnki.com.cn/Article/CJFDTOTAL-SJZA201508012.htm
[11]	张红梅, 邵元华, 龙甜甜, 等.多花黄精ISSR反应体系的建立及正交优化设计[J].安徽农业大学学报, 2012, 39(3):412-416. http://www.cnki.com.cn/Article/CJFDTOTAL-ANHU201203020.htm
[12]	中国科学院植物研究所.中国植物志:第15卷[M].北京:科学出版社, 1978:52.
[13]	徐国钧, 徐珞珊.常用中药材品种整理和质量研究:南方协作组第一册[M].福州:福建科学技术出版社, 1994:443-444.
[14]	国家中医药管理局《中华本草》编委会.中华本草:第八册[M].上海:上海科学技术出版社, 1999:143.
[15]	胡轶娟, 竺佳佳.浙江多花黄精及长梗黄精的鉴定研究[J].浙江中医药杂志, 2011, 46(8):612-613. http://www.cnki.com.cn/Article/CJFDTOTAL-ZJZZ201108058.htm
[16]	朱波, 华金渭, 刘昆, 等.珍稀药材三叶青种质资源遗传多样性的ISSR分析[J].江西农业大学学报, 2015, 37(5):914-919. http://www.cnki.com.cn/Article/CJFDTOTAL-JXND201505026.htm
[17]	张红梅. 安徽省黄精种质资源遗传多样性的ISSR分析[D]. 合肥: 安徽农业大学, 2013: 28-35. http://d.wanfangdata.com.cn/Thesis/D423708