A PCR Methodology for Detecting Eel Circovirus

LI Miao-miao

doi:10.19303/j.issn.1008-0384.2018.01.003

Volume 33 Issue 1

Mar. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(1): 12-16

LI Miao-miao. A PCR Methodology for Detecting Eel Circovirus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 12-16. doi: 10.19303/j.issn.1008-0384.2018.01.003

Citation:

LI Miao-miao. A PCR Methodology for Detecting Eel Circovirus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 12-16. doi: 10.19303/j.issn.1008-0384.2018.01.003

LI Miao-miao. A PCR Methodology for Detecting Eel Circovirus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 12-16. doi: 10.19303/j.issn.1008-0384.2018.01.003

Citation:

LI Miao-miao. A PCR Methodology for Detecting Eel Circovirus[J]. Fujian Journal of Agricultural Sciences, 2018, 33(1): 12-16. doi: 10.19303/j.issn.1008-0384.2018.01.003

PDF( 1263 KB)

A PCR Methodology for Detecting Eel Circovirus

doi: 10.19303/j.issn.1008-0384.2018.01.003

LI Miao-miao

Fujian Provincial Fishery Technical Extention Center, Fuzhou, Fujian 350002, China

Received Date: 2017-11-05
Rev Recd Date: 2017-12-24
Publish Date: 2018-01-01

Abstract

Abstract

Eel circovirus (EeCV) is one of the pathogens that cause virosis on eels.Based on the gene sequence of EeCV in GenBank, a pair of primers was selected for the development of the detection method using PCR.The optimized reaction conditions, as well as the specificity, sensitivity and repeatability of the methodology were determined to establish the preliminary testing protocol. Subsequently, 160 epidemic specimens with the suspected viral infection were collected from are as in Fujian for the determination. Out of all samples, 65 were shown to be positive, at a rate of 40.6%. It appeared that the current PCR method could be employed to detect EeCV for early disease diagnosis on eels.
- eel circovirus,
- PCR detection,
- detection methodology,
- application

FullText(HTML)

References(16)

References

[1]	VANBEURDEN S J, ENGELSMA M Y, ROOZENBURG I, et al. Viral diseases of wild and farmed European eel Anguilla anguilla with particular reference to the Netherlands[J]. Diseases of Aquatic Organisms, 2012, 101(1):69-86. doi: 10.3354/dao02501
[2]	NISHIMURA T, TOBA M, BAN F, et al. Eel rhabdovirus, EVA, EVEX and their infectivity to fishes[J]. Fish Pathology, 1981, 15(3-4):173-184. doi: 10.3147/jsfp.15.173
[3]	葛均青, 杨金先, 李友娟, 等.鳗鲡病毒性疾病病料中鳗鲡疱疹病毒的PCR检测[J].福建农业学报, 2012, 27(9):961-964. http://www.cnki.com.cn/Article/CJFDTOTAL-FJNX201209012.htm
[4]	TODD D. Circoviruses:immunosuppressive threats to avian species:a review[J]. Avian Pathology, 2000, 29(5):373-394. doi: 10.1080/030794500750047126
[5]	Todd D, Mcnulty M S. Chapter 34-Circoviridae[J]. Poultry Diseases, 2008:398-404. doi: 10.1016/B978-0-7020-2862-5.50039-8
[6]	NOTEBORN M H, BOER G F D, ROOZELAAR D J V, et al. Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle[J]. Journal of Virology, 1991, 65(6):3131-9. http://jvi.asm.org/content/65/6/3131.abstract?cited-by=yes&legid=jvi;65/6/3131
[7]	MEEHAN B M, CREELAN J L, MCNULTY M S, et al. Sequence of porcine circovirus DNA:affinities with plant circoviruses[J]. Journal of General Virology, 1997, 78(1):221. doi: 10.1099/0022-1317-78-1-221
[8]	BASSAMI M R, BERRYMAN D, WILCOX G E, et al. Psittacine beak and feather disease virus nucleotide sequence analysis and its relationship to porcine circovirus, plant circoviruses, and chicken anaemia virus[J]. Virology, 1998, 249(2):453-459. doi: 10.1006/viro.1998.9324
[9]	PHENIX K V, WESTON J H, YPELAAR I, et al. Nucleotide sequence analysis of a novel circovirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae[J]. Journal of General Virology, 2001, 82(11):2805-2809. doi: 10.1099/0022-1317-82-11-2805
[10]	LORINCZ M, CSÁGOLA A, FARKAS S L, et al. First detection and analysis of a fish circovirus[J]. Journal of General Virology, 2011, 92(8):1817-1821. doi: 10.1099/vir.0.031344-0
[11]	TARJÁN Z L, PÉNZES J J, TÓTH R P, et al. First detection of circovirus-like sequences in amphibians and novel putative circoviruses in fishes[J]. Acta Veterinaria Hungarica, 2014, 62(1):134. doi: 10.1556/AVet.2013.061
[12]	DOSZPOLY A, TARJÁN Z L, GLÁVITS R, et al. Full genome sequence of a novel circo-like virus detected in an adult European eel Anguilla anguilla showing signs of cauliflower disease[J]. Diseases of Aquatic Organisms, 2014, 109(2):107. doi: 10.3354/dao02730
[13]	LÖRINCZ M, ÁDÁMDÁN, LÁNG M, et al. Novel circovirus in European catfish (Silurusglanis)[J]. Archives of Virology, 2012, 157(6):1173. doi: 10.1007/s00705-012-1291-1
[14]	余旭平, 郑新添, 何世成, 等.鹅圆环病毒浙江永康株全基因组的克隆及序列分析[J].微生物学报, 2005, 45(6):860-864. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGJQ201521007.htm
[15]	陆承平, 方维焕, 陈金顶, 等.兽医微生物学学科发展[J].中国家禽, 2008, 30(9):31-34. http://d.wanfangdata.com.cn/Periodical_zgjq200809010.aspx
[16]	TODD D. Avian circovirus diseases:lessons for the study of PMWS[J]. Veterinary Microbiology, 2004, 98(2):169-174. doi: 10.1016/j.vetmic.2003.10.010