Induction Plant Regeneration with Stem Segments of <i>Anoectochilus</i> roxburghii (wall.) lindl. Huaye

YE Xiu-xian; CHEN Yi-quan; ZHONG Huai-qin; LIN Rong-yan; HUANG Min-ling

doi:10.19303/j.issn.1008-0384.2018.03.008

Volume 33 Issue 3

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(3): 258-262

YE Xiu-xian, CHEN Yi-quan, ZHONG Huai-qin, LIN Rong-yan, HUANG Min-ling. Induction Plant Regeneration with Stem Segments of Anoectochilus roxburghii (wall.) lindl. Huaye[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 258-262. doi: 10.19303/j.issn.1008-0384.2018.03.008

Citation:

YE Xiu-xian, CHEN Yi-quan, ZHONG Huai-qin, LIN Rong-yan, HUANG Min-ling. Induction Plant Regeneration with Stem Segments of Anoectochilus roxburghii (wall.) lindl. Huaye[J]. Fujian Journal of Agricultural Sciences, 2018, 33(3): 258-262. doi: 10.19303/j.issn.1008-0384.2018.03.008

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Induction Plant Regeneration with Stem Segments of Anoectochilus roxburghii (wall.) lindl. Huaye

doi: 10.19303/j.issn.1008-0384.2018.03.008

YE Xiu-xian^{1, 2
,},
CHEN Yi-quan³,
ZHONG Huai-qin^{1, 2},
LIN Rong-yan^{1, 2},
HUANG Min-ling^{1, 2
,
,}

1.
Institute of Crop Sciences, Fujian Academy of Agricultural Science, Flowers Research Center, Fuzhou, Fujian 350013, China
2.
Flouer Research Center, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013, China
3.
Institute of Agricultural Engineering Technology, Fujian Academy of Agricultural Science, Fuzhou, Fujian 350013, China

Received Date: 2017-11-15
Rev Recd Date: 2018-01-05
Publish Date: 2018-03-01

Abstract

Abstract

Stem segments of Anoectochilus roxburghii (wall.) lindl. Huaye were used as the explants to induce bud clumps. Varied medium (i.e., MS, B₅ or improved MS) and phytohormone (i.e., TDZ, 6-BA, KT or IBA) were applied for the tissue regeneration experiment of an orthogonal design. It was found the major factors affecting the bud multiplication to be basal medium, KT, 6-BA and TDZ. The optimal multiplication was achieved by using the improved MS+6-BA with additions of 2.0 mg·L^-1+KT 0.5 mg·L^-1+NAA 0.1 mg·L^-1+sugar 30.0 g·L^-1+agar powder 3.0 g·L^-1, and carrageenan 3.6 g·L^-1. An averaged propagation coefficient of 4.58 in 60 days was obtained. For rooting, the improved MS+6-BA with added 0.3 mg ·L^-1+AC 0.5 g·L^-1+sugar 20.0 g·L^-1+agar powder 3.6 g·L^-1 and carrageenan 3.6 g·L^-1, rendered a 100.0% rate in 60 days.
- Anoectochilus roxburghii (wall.) lindl. Huaye,
- bud clumps,
- orthogonal design,
- tissue culture

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References(9)

References

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