Molecular Identification for Sweet Potato Leaf Curl Virus Isolated in Fujian

LI Hua-wei; LIU Zhong-hua; ZHANG Hong; LIN Zhi-jian; LI Guo-liang; LI Zhao-miao; XU Yong-qing; QIU Yong-xiang; LUO Wen-bin; JI Rong-chang; TANG Hao; QIU Si-xin

doi:10.19303/j.issn.1008-0384.2018.06.015

Volume 33 Issue 6

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(6): 626-630

LI Hua-wei, LIU Zhong-hua, ZHANG Hong, LIN Zhi-jian, LI Guo-liang, LI Zhao-miao, XU Yong-qing, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, QIU Si-xin. Molecular Identification for Sweet Potato Leaf Curl Virus Isolated in Fujian[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 626-630. doi: 10.19303/j.issn.1008-0384.2018.06.015

Citation:

LI Hua-wei, LIU Zhong-hua, ZHANG Hong, LIN Zhi-jian, LI Guo-liang, LI Zhao-miao, XU Yong-qing, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, QIU Si-xin. Molecular Identification for Sweet Potato Leaf Curl Virus Isolated in Fujian[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 626-630. doi: 10.19303/j.issn.1008-0384.2018.06.015

Citation:

LI Hua-wei, LIU Zhong-hua, ZHANG Hong, LIN Zhi-jian, LI Guo-liang, LI Zhao-miao, XU Yong-qing, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, QIU Si-xin. Molecular Identification for Sweet Potato Leaf Curl Virus Isolated in Fujian[J]. Fujian Journal of Agricultural Sciences, 2018, 33(6): 626-630. doi: 10.19303/j.issn.1008-0384.2018.06.015

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Molecular Identification for Sweet Potato Leaf Curl Virus Isolated in Fujian

doi: 10.19303/j.issn.1008-0384.2018.06.015

Crop Institute Research Centre, Fujian Academy of Agricultural Sciences/Scientific Observing and Experimental Station of Tuber and Root Crops in South China, Ministry of Agricultrue, Fuzhou, Fujian 350013, China

Received Date: 2018-03-06
Rev Recd Date: 2018-05-29
Publish Date: 2018-06-01

Abstract

Abstract

Molecular characteristics of the sweet potato leaf curl virus (SPLCV) in Fujian province were studied. Specific segments of the genome were recovered, cloned and sequenced using selected primers. A sequence analysis positively confirmed the segments to be a part of SPLCV. Then, the full-length SPLCV was constructed with a pair of primers Q, and sequenced. The previously reported general purpose primers, PCRc1/PBLlv, for DNA-B were used for the subsequent amplification. The genome of SPLCV was found to be 2 827 bp containing 6 ORFs with DNA-A but not DNA-B (GenBank accession number:KT202303). The nucleotide homology between the isolated genome and the reported 6 other geminiviridae genomes was less than 85%;and, between it and SPLCV was the highest at 95.94%. In addition, the isolate shared a close genetic relationship with other isolates from Henan, Jiangsu, Zhejiang, South Korea, and the United States belonging to a same subgroup but in different branches.
- sweet potato,
- SPLCV,
- sequence analysis,
- genome

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References(17)

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