SRAP Analysis on Genetic Diversity of <i>Brassica juncea</i>

LI Yong-ping; XUE Zhu-zheng; ZHANG Shuang-zhao; WEN Qing-fang

doi:10.19303/j.issn.1008-0384.2018.09.009

Volume 33 Issue 9

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(9): 937-942

LI Yong-ping, XUE Zhu-zheng, ZHANG Shuang-zhao, WEN Qing-fang. SRAP Analysis on Genetic Diversity of Brassica juncea[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 937-942. doi: 10.19303/j.issn.1008-0384.2018.09.009

Citation:

LI Yong-ping, XUE Zhu-zheng, ZHANG Shuang-zhao, WEN Qing-fang. SRAP Analysis on Genetic Diversity of Brassica juncea[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 937-942. doi: 10.19303/j.issn.1008-0384.2018.09.009

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SRAP Analysis on Genetic Diversity of Brassica juncea

doi: 10.19303/j.issn.1008-0384.2018.09.009

LI Yong-ping^{1, 2
,},
XUE Zhu-zheng^{1, 2
,
,},
ZHANG Shuang-zhao³,
WEN Qing-fang^{1, 2}

1.
Vegetable Research Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
2.
Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
3.
Longyan Agricultural Science Institute, Longyan, Fujian 364000, China

Received Date: 2018-06-05
Rev Recd Date: 2018-07-15
Publish Date: 2018-09-01

Abstract

Abstract

An SRAP marker was applied to analyze the genetic diversity of 44 accessions of Brassica juncea(L.). Thirty-one pairs of SRAP primer combinations, which amplified stable bands with clear and higher polymorphism, were selected by a preliminary screening. A total of 286 DNA bands were amplified from the 44 accessions of B. juncea by PCR amplification.Among them, 60.49% or 173 DNA bands were polymorphic.The average number of DNA bands amplified by each pair of primer combination was 9.23. The clustering analysis by NTSYS statistical software showed that the genetic similarity coefficients were in the range of 0.61-0.89 averaging 0.75. At the coefficient of 0.67, the 44 accessions were divided into 4 groups which were identical with the result obtained by the morphological classification.
- Brassica juncea,
- SRAP,
- genetic diversity

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References(15)

References

[1]	陈材林, 周源, 周光凡, 等.中国的芥菜起源探讨[J].西南农业学报, 1992, 5(3):3-7. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK000003413624
[2]	范永红, 沈进娟, 董代文.芥菜类蔬菜产业发展现状及研究前景思考[J].农学学报, 2016, 6(2):65-71. http://d.old.wanfangdata.com.cn/Periodical/hnnykjtx-e201703045
[3]	刘佩瑛.中国芥菜[M].北京:中国农业出版社, 1996.
[4]	刘淑艳, 刘忠松, 官春云.芥菜型油菜种质资源研究进展[J].植物遗传资源学报, 2007, 8(3):351-358. doi: 10.3969/j.issn.1672-1810.2007.03.022
[5]	刘忠松, 官春云, 李木旬, 等.甘蓝型油菜与芥菜型油菜种间杂交研究[J].中国油料作物学报, 2001, 23(2):82-87. doi: 10.3321/j.issn:1007-9084.2001.02.022
[6]	顾祥昆, 李菲, 张淑江, 等.芥菜游离小孢子培养技术研究[J].中国蔬菜, 2013(12):23-30. http://d.old.wanfangdata.com.cn/Periodical/zgsc201312006
[7]	蒋先军, 骆永明, 赵其国.重金属污染土壤的植物修复研究Ⅲ.金属富集植物Brassica juncea对锌镉的吸收和积累[J].土壤学报, 2002, 39(5)71-74. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=trxb200205007
[8]	张雁, 黄丽慧, 陈于陇, 等.不同品种芥菜发酵过程中亚硝酸盐变化规律的研究[J].现代食品科技, 2013, 29(9):2152-2157. http://www.cnki.com.cn/Article/CJFDTOTAL-GZSP201309021.htm
[9]	孙涛, 张玉秀, 柴团耀.印度芥菜(Brassica juncea L.)重金属耐性机理研究进展[J].中国生态农业学报, 2011, 19(1):226-234. http://d.old.wanfangdata.com.cn/Periodical/stnyyj201101040
[10]	乔爱民, 刘佩瑛, 雷建军, 芥菜16个变种的RAPD研究[J].植物学报, 1998, 40(10):915-921. doi: 10.3321/j.issn:1672-9072.1998.10.006
[11]	李宁, 司军, 任雪松, 等.芥菜种质资源遗传多样性和亲缘关系的SRAP分析[J].中国蔬菜, 2014(5):26-33. doi: 10.3969/j.issn.1000-6346.2014.05.005
[12]	刘佩瑛.中国芥菜[M].北京:中国农业出版社, 1995:56-59.
[13]	付杰.中国芥菜遗传多样性与亲缘关系的研究[D].杭州: 浙江大学, 2005.
[14]	齐晓花.中国芥菜系统进化研究及重要数量性状的遗传分析[D].杭州: 浙江大学, 2009.
[15]	宋明, 刘婷, 汤青林, 等.芥菜种质资源的RAPD和ISSR分析.园艺学报, 2009, 36(6):835-842. doi: 10.3321/j.issn:0513-353X.2009.06.009