Isolation and Expression of Peroxidase <i>POD</i>1 Gene from <i>Cucurbita pepo</i>

HUANG Li-fang; LIU Jian-ting; WANG Bin; WEN Qing-fang; ZHU Hai-sheng; LIN Bi-ying

doi:10.19303/j.issn.1008-0384.2018.09.010

Volume 33 Issue 9

Mar. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2018 > 33(9): 943-949

HUANG Li-fang, LIU Jian-ting, WANG Bin, WEN Qing-fang, ZHU Hai-sheng, LIN Bi-ying. Isolation and Expression of Peroxidase POD1 Gene from Cucurbita pepo[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 943-949. doi: 10.19303/j.issn.1008-0384.2018.09.010

Citation:

HUANG Li-fang, LIU Jian-ting, WANG Bin, WEN Qing-fang, ZHU Hai-sheng, LIN Bi-ying. Isolation and Expression of Peroxidase POD1 Gene from Cucurbita pepo[J]. Fujian Journal of Agricultural Sciences, 2018, 33(9): 943-949. doi: 10.19303/j.issn.1008-0384.2018.09.010

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Isolation and Expression of Peroxidase POD1 Gene from Cucurbita pepo

doi: 10.19303/j.issn.1008-0384.2018.09.010

HUANG Li-fang^{1, 2, 3},
LIU Jian-ting^{2, 3},
WANG Bin^{2, 3},
WEN Qing-fang^{2, 3},
ZHU Hai-sheng^{2, 3
,
,},
LIN Bi-ying^{1
,
,}

1.
College of Horticulture. Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
3.
Vegetable Research Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2018-06-08
Rev Recd Date: 2018-08-07
Publish Date: 2018-09-01

Abstract

Abstract

A functional annotation as peroxidase (POD) gene (i.e., c47620.graph_c0) was screened from the transcriptomic sequencing of Cucurbita pepo. It was found that thesequence length was 1 245 bp, including a 996bp open reading frame (ORF) encoded a protein of 331 amino acids. The protein had a predicted molecular weight of 35.70 kDa and a hypothetical isoelectric point of 5.40. The similarity of the proteins to the homologous proteins of cucumber, melon and balsam pear were 84%, 84% and 81%, respectively, witha highly conservative nature. It was designated as CpPOD1 gene with a GenBank accession code of MF988285. The RTqPCR revealed the geneto betissue-specific, andits expression in C. pepo highest in the leaves, followed by roots and flowers. Under low temperature and dim light, the CpPOD1 expression was up-regulated over time. It suggested that the gene expression correlatedwith the low temperature/dim light stress as C. pepo regulated the downstream responses. This study could lead to further understanding of the function of CpPOD1 gene inzucchinion cold-and-low-lighttolerance and the regulation mechanism providing evidence and theoretical basis for improved breeding of C. pepo.
- Cucurbita pepo,
- POD,
- RNA-Seq,
- qRT-PCR

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References(31)

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