Pathogen Identification and Botanical Fungicides for Soft Rot Disease on Guichang Kiwifruit

SHI Jin-qiao; LONG You-hua; LI Xiao-qian; MO Fei-xu; RAN Fei; HUANG Ya-xin

doi:10.19303/j.issn.1008-0384.2019.03.012

Volume 34 Issue 3

Jun. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(3): 331-337

SHI Jin-qiao, LONG You-hua, LI Xiao-qian, MO Fei-xu, RAN Fei, HUANG Ya-xin. Pathogen Identification and Botanical Fungicides for Soft Rot Disease on Guichang Kiwifruit[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 331-337. doi: 10.19303/j.issn.1008-0384.2019.03.012

Citation:

SHI Jin-qiao, LONG You-hua, LI Xiao-qian, MO Fei-xu, RAN Fei, HUANG Ya-xin. Pathogen Identification and Botanical Fungicides for Soft Rot Disease on Guichang Kiwifruit[J]. Fujian Journal of Agricultural Sciences, 2019, 34(3): 331-337. doi: 10.19303/j.issn.1008-0384.2019.03.012

Citation:

PDF( 1800 KB)

Pathogen Identification and Botanical Fungicides for Soft Rot Disease on Guichang Kiwifruit

doi: 10.19303/j.issn.1008-0384.2019.03.012

SHI Jin-qiao^1
,,
LONG You-hua^{1, 2
,
,},
LI Xiao-qian¹,
MO Fei-xu^{1, 2},
RAN Fei¹,
HUANG Ya-xin³

1.
College of Agricultural, Guizhou University, Guiyang, Guizhou 550025, China
2.
Engineering and Technology Research Center of Kiwifruit, Guizhou University, Guiyang, Guizhou 550025, China
3.
Xiuwen County Kiwifruit Industry Development Board of Guizhou Province, Xiuwen, Guizhou 550200, China

Received Date: 2018-11-22
Rev Recd Date: 2019-02-27
Publish Date: 2019-03-28

Abstract

Abstract

Objective Pathogens that cause the soft rot disease on Guichang kiwifruit and potential botanical fungicides for the disease control were investigated. Method Suspected microbes were isolated using tissue culture and verified as the causation pathogens by means of artificial infection. The pathogens were identified morphologically and molecular biologically. In an indoor toxicity test using the mycelium growth method, 6 selected botanical fungicides were applied on the identified pathogenic isolates to determine their potencies for the disease control. Result Among the 8 isolated strains, RF2 and RF2-4 induced the soft rot disease on kiwifruits. The BLAST comparison on NCBI of the rDNA-ITS sequences of these strains suggested that RF2 was Botryosphaeria dothidea with a perfect match, and RF2-4 Phomopsis sp. with a 99% homology. The morphological analysis further confirmed the identifications. The EC₅₀ of the organic fungicides were found for 0.5% matrine AS to be 0.442 mg·L^-1 against B. dothidea and 0.322 mg·L^-1 against Phomopsis sp.; and for 0.3% eugenol SL, 0.680 mg·L^-1 against B. dothidea and 0.301 mg·L^-1 against Phomopsis sp. The potencies were greater than other botanical fungicides tested. Conclusion B. dothidea and phomopsis sp.were positively identified as the pathogens that caused the soft rot disease on kiwifruits; and as botanical fungicides, 0.5% matrine AS and 0.3% eugenol SL seemed most effective for the disease control.
- kiwifruit,
- botanical fungicides,
- soft rot disease,
- toxicity determination,
- green method for disease control

FullText(HTML)

References(18)

References

[1]	马松涛, 宋晓斌, 张学武, 等.猕猴桃花果病害研究现状及趋势[J].西北林学院学报, 2000, 15(3):86-90. doi: 10.3969/j.issn.1001-7461.2000.03.019 MA S T, SONG X B, ZHANG X W, et al. Present situation and trend of the researches on flower and fruit diseases of kiwifruit[J]. Journal of Northwest Forestry University, 2000, 15(3): 86-90.(in Chinese) doi: 10.3969/j.issn.1001-7461.2000.03.019
[2]	韩礼星, 黄贞光, 李明, 等.加入WTO后我国猕猴桃产业的发展策略[J].果树学报, 2003, 20(3):218-223. http://d.old.wanfangdata.com.cn/Periodical/gskx200303016 HAN L X, HUANG Z G, LI M, et al. On the strategies for development of kiwifruit industry in China after accessed to WTO[J]. Journal of Fruit Science, 2003, 20(3): 218-223.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/gskx200303016
[3]	HAWTHORNE B T, REES-GEORGE J, SAMUELS G J. Fungi associated with leaf spots and post-harvest fruit rots of kiwifruit (Actinidia chinensis) in New Zealand[J]. New Zealand Journal of Botany, 1982, 20(2):143-150. doi: 10.1080/0028825X.1982.10428835
[4]	丁爱冬, 于梁, 石蕴莲.猕猴桃采后病害鉴定和侵染规律研究[J].植物病理学报, 1995(2):149-153. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199500674990 DING A D, YU L, SHI Y L. Studies on the identification of pathogenic fungi and their ways of post-harvest rot disease infection on kiwifruit[J]. Acta Phytopathologica Sinica, 1995 (2): 149-153.(in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199500674990
[5]	张承, 李明, 龙友华, 等.采前喷施壳聚糖复合膜对猕猴桃软腐病的防控及其保鲜作用[J].食品科学, 2016, 37(22):274-281. doi: 10.7506/spkx1002-6630-201622042 ZHANG C, LI M, LONG Y H, et al. Control of soft rot in kiwifruit by pre-harvest application of chitosan composite coating and its effect on preserving and improving kiwifruit quality[J]. Food Science, 2016, 37(22): 274-281.(in Chinese) doi: 10.7506/spkx1002-6630-201622042
[6]	黎晓茜, 曾彬, 尹显慧, 等.修文县猕猴桃腐烂病病原鉴定及防治药剂筛选[J].中国南方果树, 2016, 45(5):101-104. http://d.old.wanfangdata.com.cn/Periodical/zgnfgs201605026 LI X X, ZENG B, YIN X H, et al. Identification of pathogenic fungi causing fruit rot of kiwifruit in Xiuwen county and the toxicity assay of fungicites[J]. South China Fruits, 2016, 45(5): 101-104.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgnfgs201605026
[7]	吴文能, 张起, 雷霁卿, 等.贵长猕猴桃软腐病病原菌分离鉴定及抑菌药剂筛选[J].北方园艺, 2018(16):47-54. http://d.old.wanfangdata.com.cn/Periodical/bfyany201816008 WU W N, ZHANG Q, LEI J Q, et al. Identification and pharmaceutical screening of kiwifruit soft rot disease on 'Guichang' gooseberry[J]. Northern Horticulture, 2018 (16): 47-54.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201816008
[8]	潘慧, 胡秋舲, 张胜菊, 等.贵州六盘水市猕猴桃病害调查及病原鉴定[J].植物保护, 2018, 44(4):125-131. http://d.old.wanfangdata.com.cn/Periodical/zwbh201804023 PAN H, HU Q L, ZHANG S J. et al. Kiwifruit disease investigation and pathogen identification in liupanshui city, Guizhou province[J]. Plant Protection, 2018, 44(4): 125-131.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwbh201804023
[9]	李诚, 蒋军喜, 冷建华, 等. 6种杀菌剂对猕猴桃主要腐烂病菌的室内毒力测定[J].中国南方果树, 2012, 41(1):27-29. http://d.old.wanfangdata.com.cn/Periodical/zgnfgs201201007 LI C, JIANG J X, LENG J H, et al. Indoor toxicity test of six fungicides to main pathogenic fungi causing fruit rot of kiwifruit[J]. South China Fruits, 2012, 41(1): 27-29.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgnfgs201201007
[10]	刘达富, 王井田, 陆雪峰, 等.猕猴桃果实腐烂病田间药效试验[J].浙江林业科技, 2015, 35(2):70-73. doi: 10.3969/j.issn.1001-3776.2015.02.016 LIU D F, WANG J T, LU X F, et al. Field experiment on fungicides on fruit rot of actinidia chinensis[J]. Journal of Zhejiang Forestry Science and Technology, 2015, 35(2): 70-73.(in Chinese) doi: 10.3969/j.issn.1001-3776.2015.02.016
[11]	王小洁, 李士谣, 李亚巍, 等.猕猴桃软腐病病原菌的分离鉴定及其防治药剂筛选[J].植物保护学报, 2017, 44(5):826-832. http://d.old.wanfangdata.com.cn/Periodical/zwbhxb201705016 WANG X J, LI S Y, LI Y W, et al. Pathogen identification of kiwifruit soft rot and fungicide screening for control of the disease[J]. Journal of Plant Protection, 2017, 4(5): 826-832.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zwbhxb201705016
[12]	李诚, 蒋军喜, 冷建华, 等.猕猴桃枝枯病病原菌鉴定[J].北方园艺, 2013(24):130-133. http://d.old.wanfangdata.com.cn/Periodical/bfyany201324038 LI C, JIANG J X, LENG J H, et al. Identification of the pathogen causing shoot blight of Kiwifruit[J]. Northern Horticulture, 2013(24): 130-133.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201324038
[13]	凌丹燕.蓝莓主要真菌病害的分离鉴定与防治研究[D].金华: 浙江师范大学, 2016. LING D Y. The study of isolation and identification of major fungal diseases of blueberry and disease control[D]. Jinhua: Zhejiang Normal University, 2016.(in Chinese)
[14]	何军, 马志卿, 张兴.植物源农药概述[J].西北农林科技大学学报(自然科学版), 2006, 34(9):79-85. doi: 10.3321/j.issn:1671-9387.2006.09.017 HE J, MA Z Q, ZHANG X. Review of botanical pesticide[J]. Journal of Northwest A & F University(Natural Science Edition), 2006, 34(9): 79-85.(in Chinese) doi: 10.3321/j.issn:1671-9387.2006.09.017
[15]	陈昂.五种植物源农药的环境毒性评价[D].长沙: 中南大学, 2010. CHEN A. Environmental toxicity evaluation of five botanical pesticides[D]. Changsha: Central South University, 2010.(in Chinese)
[16]	汤丽梅, 周清, 吴尚, 等.大蒜素对猕猴桃果实致病菌的抑制活性及贮藏防效研究[J].中国南方果树, 2014, 43(4):15-18. http://d.old.wanfangdata.com.cn/Periodical/zgnfgs201404004 TONG L M, ZHOU Q, WU S, et al. Study on inhibitory activity of allicin to pathogens of kiwifruit and its efficacy on fruit storage[J]. South China Fruits, 2014, 43(4): 15-18.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgnfgs201404004
[17]	王强, 冯岩, 何小静, 等.植物源杀菌剂的筛选[J].福建农业学报, 2012, 27(11):1246-1249. doi: 10.3969/j.issn.1008-0384.2012.11.019 WANG Q, FENG Y, HE X J, et al. Screening of the botanical fungicides[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1246-1249.(in Chinese) doi: 10.3969/j.issn.1008-0384.2012.11.019
[18]	周军, 赵杰.植物性杀菌剂对桃炭疽病菌和桃果腐病菌的毒力测定[J].上海农业科技, 2018(5):113. doi: 10.3969/j.issn.1001-0106.2018.05.055 ZHOU J, ZHAO J. Toxicity determination of botanical fungicides to anthracnose and fruit rot on Peach[J]. Shanghai Agricultural Science and Technology, 2018 (5): 113.(in Chinese) doi: 10.3969/j.issn.1001-0106.2018.05.055