Identification of <i>Grifola frondosa</i> by Antagonistic Test and ISSR

YUAN Bin; KE Li-na; HUANG Yi-ning; ZHANG Zhi-hong; LIAN Yan-ping; ZHANG Dan-feng

doi:10.19303/j.issn.1008-0384.2019.04.003

Volume 34 Issue 4

Jul. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(4): 393-399

YUAN Bin, KE Li-na, HUANG Yi-ning, ZHANG Zhi-hong, LIAN Yan-ping, ZHANG Dan-feng. Identification of Grifola frondosa by Antagonistic Test and ISSR[J]. Fujian Journal of Agricultural Sciences, 2019, 34(4): 393-399. doi: 10.19303/j.issn.1008-0384.2019.04.003

Citation:

YUAN Bin, KE Li-na, HUANG Yi-ning, ZHANG Zhi-hong, LIAN Yan-ping, ZHANG Dan-feng. Identification of Grifola frondosa by Antagonistic Test and ISSR[J]. Fujian Journal of Agricultural Sciences, 2019, 34(4): 393-399. doi: 10.19303/j.issn.1008-0384.2019.04.003

Citation:

PDF( 1451 KB)

Identification of Grifola frondosa by Antagonistic Test and ISSR

doi: 10.19303/j.issn.1008-0384.2019.04.003

1.
Zhangzhou Institute of Agricultural Science, Zhangzhou, Fujian 363005, China
2.
Zhangzhou Vocational and Technical College, Zhangzhou, Fujian 363000, China
3.
Minnan Normal University, Zhangzhou, Fujian 363000, China

Received Date: 2018-11-28
Rev Recd Date: 2019-03-19
Publish Date: 2019-04-28

Abstract

Abstract

Objective To reliably identify strains of Grifola frondosa and precisely determine the genetic relationship among them. Method The identification and genetic diversity analysis on 11 strains of G. frondosa were carried out by the combined use of the antagonistic challenging test and ISSR. Result The antagonistic test on the strains demonstrated apparent differences on their responses. Presence or absence of antagonism allowed the classification of the strains into 4 distinct groups. Meanwhile, from the ISSR analysis, when the similarity coefficient reached 0.87, the strains were clustered into 5 groups. They were Group 1 that included Strain #1 (Shijiazhuang ash tree flower), Strain #2 (stage ash), Strain #10 (XG-W), Strain #5 (Gr0001+2), Strain #6 (CN), and Strain #7 (snow circle); Group 2, Strain #11 (CN-9); Group 3, Strain #4 (Gr0003); Group 4, Strain #8 (H) and Strain #9 (Hokto); and, Group 5, Strain #3 (Gr20). Conclusion It appeared that Strain #1 and Strain #2 belonged to a same species; so were Strains #5, Strain #6 and Strain #7, and Strains #8 and Strain #9. At the similarity coefficient of 0.8, Strain #4 could be clustered with Strain #8 and Strain #9 with slight differences indicating a distance on genetic relationship among them. Strain #3 had a lowest coefficient of 0.34, therefore, it was most remotely related to all.
- antagonistic effect,
- ISSR,
- Grifola frondosa,
- identification,
- cluster analysis

FullText(HTML)

References(34)

References

[1]	刘佳, 包海鹰, 图力古尔.灰树花化学成分及药理活性研究进展[J].菌物研究, 2018, 16(3):150-157. http://d.old.wanfangdata.com.cn/Periodical/jwyj201803004 LIU J, BAO H Y, BAU Tolgor. Chemical Constituents and Pharmacological Activities of Grifola frondosa[J]. Journal of Fungal Research, 2018, 16(3):150-157. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jwyj201803004
[2]	郭健.灰树花标准菌株库的建立及优良菌株筛选[D].长春: 吉林农业大学, 2016. GUO J.Establishment of standard strain Bank of Grifola frondosa and screening of excellent strains[D]. Changchun: Jilin Agricultural University, 2016. (in Chinese)
[3]	杨杨.灰树花EST-SSR引物开发及遗传多样性研究[D].长春: 吉林农业大学, 2016. YANG Y.Development of EST-SSR Primers and Study on Genetic Diversity of Grifola frondosa[D]. Changchun: Jilin Agricultural University, 2016. (in Chinese)
[4]	曹鸿雁.黑木耳等9种食用菌的种质资源分子鉴定[D].福州: 福建农林大学, 2009. CAO H Y. Molecular Identification of Germplasm Resources of 9 Edible Fungi such as Auricularia auricula[D]. Fuzhou: Fujian Agricultural and Forestry University, 2009. (in Chinese)
[5]	田君琪, 韩晓伟.灰树花对免疫系统的影响[J].吉林中医药, 2018, 38(10):1203-1205. http://d.old.wanfangdata.com.cn/Periodical/jlzyy201810026 TIAN J Q, HAN X W. Effect of Grifola frondosa on immune system[J].Jilin Traditional Chinese Medicine, 2018, 38(10):1203-1205. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jlzyy201810026
[6]	刘佳, 包海鹰, 图力古尔, 等.灰树花提取物的体内抗肿瘤及免疫活性研究[J/OL].西北农林科技大学学报(自然科学版), 2019(4): 1-3[2018-11-27].https://doi.org/10.13207/j.cnki.jnwafu.2019.04.014. LIU J, BAO H Y, BAU Tolgor, et al. Anti-tumor and Immune Activity of Grifola frondosa Extract in Vitro[J/OL].Journal of Northwest A & F University(Natural Science Edition), 2019(4): 1-3[2018-11-27].https://doi.org/10.13207/j.cnki.jnwafu.2019.04.014. (in Chinese)
[7]	胡卫红.灰树花工厂液体菌种生产工艺的研究[J].科学中国人, 2016(20):12. http://epub.cnki.net/grid2008/detail.aspx?filename=KXZG201620008&dbname=CJFN2016 HU W H. Study on Production Technology of Liquid Strains in Grifola frondosa Factory[J]. Scientific Chinese, 2016(20):12. (in Chinese) http://epub.cnki.net/grid2008/detail.aspx?filename=KXZG201620008&dbname=CJFN2016
[8]	张立霞, 林绪涛, 李军, 等.灰树花多糖及其复合多糖对肺纤维化大鼠的作用[J].中国老年学杂志, 2018, 38(17):4242-4245. doi: 10.3969/j.issn.1005-9202.2018.17.054 ZHANG L X, LIN X T, LI J, et al.Effects of Grifola frondosa polysaccharide and its compound polysaccharide on pulmonary fibrosis in rats[J].Chinese Journal of Gerontology, 2018, 38(17):4242-4245. (in Chinese) doi: 10.3969/j.issn.1005-9202.2018.17.054
[9]	陈沛, 刘会平, 孙娜新, 等.灰树花多糖的分离纯化及其体外抗肿瘤活性[J].现代食品科技, 2018, 34(6):107-114. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gzspgykj201806015 CHEN P, LIU H P, SUN N X, et al. Isolation and Purification of Polysaccharide from Grifola frondosa and Its in vitro Antitumor Activity[J]. Modern Food Science and Technology, 2018, 34(6):107-114. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=gzspgykj201806015
[10]	张医芝.灰树花菌丝体液体深层发酵工艺优化及降血糖和抗肿瘤活性研究[D].长春: 吉林大学, 2017. ZHANG Y Z. Study on Optimization of submerged fermentation of Grifola frondosa mycelium and its hypoglycemic and antineoplastic activities[D]. Changchun: Jilin University, 2017. (in Chinese)
[11]	郭家瑞.灰树花多糖高产菌株的选育及其发酵特性研究[D].郑州: 河南工业大学, 2011. GUO J R. Breeding and Fermentation Characteristics of Grifola frondosa Polysaccharide High-yielding Strain[D].Zhengzhou: Henan University of Technology, 2011. (in Chinese)
[12]	刘振伟, 史秀娟.灰树花杂交良种的选育[J].山东农业科学, 2005(1):27-28, 32. doi: 10.3969/j.issn.1001-4942.2005.01.008 LIU Z W, S X J. Breeding of improved hybrid Grifola frondosa[J]. Shandong Agricultural Science, 2005(1):27-28, 32. (in Chinese) doi: 10.3969/j.issn.1001-4942.2005.01.008
[13]	陈世通, 李荣春.食用菌育种方法的研究现状、存在的问题及展望[J].安徽农业科学, 2012, 40(10):5850-5852. doi: 10.3969/j.issn.0517-6611.2012.10.049 CHEN S T, LI R C.Present Research on Breeding Methods of Edible Fungi and the Problems in It as Well as the Prosipects[J]. Anhui Agricultural Science, 2012, 40(10):5850-5852. (in Chinese) doi: 10.3969/j.issn.0517-6611.2012.10.049
[14]	刘盛荣, 张维瑞, 柯斌榕, 等.基于拮抗试验的杏鲍菇菌株分类研究[J].食药用菌, 2015, 23(1):33-36. LIU S R, ZHANG W R, KE B R, et al.Study on classification of Pleurotus eryngii strains based on antagonism test[J].Edible and Medicinal Mushrooms, 2015, 23(1):33-36. (in Chinese)
[15]	李维焕, 蔡德华, 郑芳, 等.秀珍菇菌株的亲缘关系分析[J].食品科学, 2010, 31(17):267-271. http://d.old.wanfangdata.com.cn/Periodical/spkx201017060 LI W H, CAI D H, ZHENG F, et al.Analysis of the Phylogenetic Relationships of Pleurotus pulmonarius Strains[J]. Food Science, 2010, 31(17):267-271. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/spkx201017060
[16]	唐传红, 张劲松, 陈明杰, 等.利用拮抗试验和RAPD对灵芝属菌株进行分类研究[J].微生物学通报, 2005(5):72-76. doi: 10.3969/j.issn.0253-2654.2005.05.015 TANG C H, ZHANG J S, CHEN M J, et al.Study on Classification of Strains of Ganotterma by Anatagonistic Effect and Rapd[J]. Microbiology China, 2005(5):72-76. (in Chinese) doi: 10.3969/j.issn.0253-2654.2005.05.015
[17]	郑亚凤.高多糖的姬松茸和灰树花品种及其生物活性的研究[D].福州: 福建农林大学, 2008. ZHENG Y F. Studies on the Varieties of Agaricus blazei Murrill and Grifola frondosa with High Polysaccharides and Their Biological Activities[D]. Fuzhou: Fujian Agricultural and Forestry University, 2008. (in Chinese)
[18]	李辉平.ISSR在食用菌遗传多样性研究中的应用[D].北京: 中国农业科学院, 2007. LI H P. Application of ISSR in the Study of Genetic Diversity of Edible Fungi[D]. Beijing: Chinese Academy of Agricultural Sciences, 2007. (in Chinese)
[19]	孟虎, 孙国琴, 睢韡, 等.ISSR技术在食用菌研究上的应用[J].北方园艺, 2016(5):207-210. http://d.old.wanfangdata.com.cn/Periodical/bfyany201605053 MENG H, SUN G Q, SUI W, et al.The Application of ISSR in Edible Fungi Research[J]. Northern Horticulture, 2016(5):207-210. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201605053
[20]	贾媛, 李忠海, 黎继烈, 等.ISSR指纹图谱技术在种质资源研究上的应用[J].河北农业科学, 2013, 17(5):63-68. doi: 10.3969/j.issn.1088-1631.2013.05.019 JIA Y, LI Z H, LI J L, et al.The Application of ISSR Fingerprinting Technology in Germplasm Resource Research[J]. Journal of Hebei Agricultural Sciences, 2013, 17(5):63-68. (in Chinese) doi: 10.3969/j.issn.1088-1631.2013.05.019
[21]	王春晖, 尹永刚, 胡汝晓.基于ISSR和RAPD标记的八株灰树花栽培菌株遗传多样性分析[J].食用菌学报, 2013, (4):1-5. doi: 10.3969/j.issn.1005-9873.2013.04.001 WANG C H, YIN Y G, HU R X, et al.Analysis of genetic diversity of eight Grifola frondosa cultivated strains based on ISSR and RAPD markers[J].Journal of Edible Fungi, 2013, (4):1-5. (in Chinese) doi: 10.3969/j.issn.1005-9873.2013.04.001
[22]	温志强, 熊芳, 陈吉娜, 等.分子标记鉴别灰树花种质资源的研究[J].热带作物学报, 2011, 32(7):1330-1336. doi: 10.3969/j.issn.1000-2561.2011.07.028 WEN Z Q, XIONG F, CHEN J N, et al. Identification of Grifola frondosa Germplasm Resources by Molecular Markers[J].Journal of Tropical Crops, 2011, 32(7):1330-1336. (in Chinese) doi: 10.3969/j.issn.1000-2561.2011.07.028
[23]	顾万春.统计遗传学[M].北京:科学出版社, 2004:179. GU W C. Statistical Genetics[M]. Beijing:Science Press, 2004:179. (in Chinese)
[24]	岳万松.云南牛肝菌的分子鉴定及其多糖抗氧化性研究[D].昆明: 云南民族大学, 2015. YUE W S. Molecular identification of Boletus yunnanensis and antioxidant activity of its polysaccharides[D].Kunming: Yunnan Nationalities University, 2015. (in Chinese)
[25]	戚元成, 张小强, 高玉千, 等.中国栽培糙皮侧耳品种体细胞不亲和性实验与RAPD分析结果的比较[J].菌物学报, 2010, 29(3):379-388. http://d.old.wanfangdata.com.cn/Periodical/jwxt201003010 QI Y C, ZHANG X Q, GAO Y Q, et al.Comparison between somatic incompatibility and RAPD analysis of cultivated Pleurotus ostreatus in China[J]. Mycosystema, 2010, 29(3):379-388. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jwxt201003010
[26]	宿红艳, 王磊, 明永飞, 等. ISSR分子标记技术在金针菇菌株鉴别中的应用[J].生态学杂志, 2008, 27(10):1725-1728. http://d.old.wanfangdata.com.cn/Periodical/stxzz200810015 SU H Y, WANG L, MING Y F, et al.Application of ISSR in identifying Flammulina velutipes strains[J]. Journal of Ecology, 2008, 27(10):1725-1728. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/stxzz200810015
[27]	张介驰, 马庆芳, 张丕奇, 等.用ISSR分子标记鉴别东北地区黑木耳生产菌株的研究[J].菌物学报, 2007(4):534-538. http://d.old.wanfangdata.com.cn/Periodical/jwxt200704008 ZHANG J C, MA Q F, ZHANG P Q, et al. Identification of Auricularia auricula producing strains in Northeast China by ISSR molecular markers[J].Journal of Mycology, 2007(4):534-538. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jwxt200704008
[28]	秦莲花, 宋春艳, 谭琦, 等.用ITS和ISSR分子标记技术鉴别香菇生产用种[J].菌物学报, 2006, 25(1):94-100. http://d.old.wanfangdata.com.cn/Periodical/jwxt200601015 QIN L H, SONG C Y, TANG Q, et al. Identification of Lentinus edodes varieties by ITS and ISSR molecular markers[J]. Mycosystema, 2006, 25(1):94-100. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/jwxt200601015
[29]	王守现, 刘宇, 耿小丽, 等.北京地区8个白金针菇主栽菌株的鉴别研究[J].华北农学报, 2009, 24(1):219-222. http://d.old.wanfangdata.com.cn/Periodical/hbnxb200901048 WANG S X, LIU Y, GENG X L, et al. Identification of eight main strains of Flammulina velutipes in Beijing[J]. Acta Agriculturae Boreali-Sinica, 2009, 24(1):219-222. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/hbnxb200901048
[30]	王小艳, 王春晖, 姜性坚, 等.杏鲍菇多孢分离菌株ISSR、RAPD分子标记及体细胞不亲和性分析[J].中国食用菌, 2015, 34(3):57-60, 65. http://d.old.wanfangdata.com.cn/Periodical/zgsyj201503014 WANG X Y, WANG C H, JIANG X J, et al. Analysis on Multi-spore Isolated Pleurotus eryngii Using ISSR and RAPD Molecular[J]. Edible Fungi of Ching, 2015, 34(3):57-60, 65. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zgsyj201503014
[31]	周洁. ISSR分子标记在姬菇杂交菌株鉴定中的应用[D].雅安: 四川农业大学, 2010. ZHOU J. Application of ISSR Molecular Markers in Identification of Hybrid Strains of Agaricus blazei[D]. Yaan: Sichuan Agricultural University, 2010. (in Chinese)
[32]	林戎斌, 张慧, 林陈强, 等. ISSR、RAPD和SRAP分子标记技术在姬松茸菌株鉴定上的应用比较[J].福建农业学报, 2012, 27(2):149-152. doi: 10.3969/j.issn.1008-0384.2012.02.008 LIN R B, ZHANG H, LIN C Q, et al.DNA Fingerprinting and Genetic Diversity of Cultivated Strains of Agaricus blazei Murill[J]. Fujian Journal of Agricultural Sciences, 2012, 27(2):149-152. (in Chinese) doi: 10.3969/j.issn.1008-0384.2012.02.008
[33]	杨军, 徐吉, 宋一鸣, 等.耐高温型灰树花菌株筛选及供试灰树花亲缘关系分析[J].基因组学与应用生物学, 2016, 35(2):396-405. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jyzxyyyswx201602026 YANG J, XU J, SONG Y M, et al.Screen of Grifola frondosa Thermotolerant Strains and Analysis of the Genetic Relationship of Candidate Strains[J]. Genomics and Applied Biology, 2016, 35(2):396-405. (in Chinese) http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=jyzxyyyswx201602026
[34]	王守现, 刘宇, 张英春, 等.六个灰树花菌株遗传多样性分析[J].北方园艺, 2010(1):201-204. http://d.old.wanfangdata.com.cn/Periodical/bfyany201001083 WANG S X, LIU Y, ZHANG Y C, et al.The analysis of genetic diversity of six Grifola frondosa Strains[J]. Northern Horticulture, 2010(1):201-204. (in Chinese) http://d.old.wanfangdata.com.cn/Periodical/bfyany201001083