Effects of Bt Rice on the Structure of Culturable Bacteria in the Gut of <i>Nilaparvata lugens</i> Nymphae

LIN Sheng; LI Qiang; XIA Xiao-feng; YOU Min-sheng

doi:10.19303/j.issn.1008-0384.2019.07.008

Volume 34 Issue 7

Jul. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(7): 802-809

LIN Sheng, LI Qiang, XIA Xiao-feng, YOU Min-sheng. Effects of Bt Rice on the Structure of Culturable Bacteria in the Gut of Nilaparvata lugens Nymphae[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 802-809. doi: 10.19303/j.issn.1008-0384.2019.07.008

Citation:

LIN Sheng, LI Qiang, XIA Xiao-feng, YOU Min-sheng. Effects of Bt Rice on the Structure of Culturable Bacteria in the Gut of Nilaparvata lugens Nymphae[J]. Fujian Journal of Agricultural Sciences, 2019, 34(7): 802-809. doi: 10.19303/j.issn.1008-0384.2019.07.008

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Effects of Bt Rice on the Structure of Culturable Bacteria in the Gut of Nilaparvata lugens Nymphae

doi: 10.19303/j.issn.1008-0384.2019.07.008

LIN Sheng^{1, 2, 3, 4
,},
LI Qiang^{1, 2, 3, 4},
XIA Xiao-feng^{1, 2, 3, 4},
YOU Min-sheng^{1, 2, 3, 4
,
,}

1.
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
3.
Joint International Research Laboratory of Ecological Pest Control, Ministry of Education, Fuzhou, Fujian 350002, China
4.
Key Laboratory of Integrated Pest Management of Fujian and Taiwan, China Ministry of Agriculture, Fuzhou, Fujian 350002, China

Received Date: 2018-12-10
Rev Recd Date: 2019-06-09
Publish Date: 2019-07-20

Abstract

Abstract

Objective The present study aims to explore the effects of Bt rice on gut bacterial structure of the Nilaparvata lugens nymphae and to provide evidence for rational safety assessment of Bt rice. Method Gut bacteria from BPH feeding on non-transgenic rice (CK) and transgenic rice (Bt) were isolated using traditional culture methods, and were identified using 16s rDNA. The composition of isolates was compared between these two treatments. Results The results showed that seven bacterial strains were isolated from the gut of N. lugens feeding on either CK or Bt rice. Three phyla and five genera of the gut bacteria were identified from N. lugens feeding on CK rice, while two phyla and four genera of the gut bacteria from N. lugens feeding on Bt rice. The taxa of bacteria identified from the guts of N. lugens either feeding on CK rice or Bt rice were Proteobateria (including the genus of Pantoea) and Firmicutes (including the genus of Staphylococcus). Herbaspirillum Acinetobacter and Microbacterium were specific genera from the CK treatment, while Enterobacter, and Burkholderia from the Bt treatment. Conclusion The composition of culturable bacteria in the N. lugens gut was different beween the CK treatment and the Bt treatment. The findings provide an important foundation for further studies on the function of symbiotic bacteria in N. lugens, and the effects of transgenic rice on the gut bacteria of non-target species.
- Nilaparvata lugens,
- genetically modified rice,
- gut bacteria,
- non-target effect,
- biosafety

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References(30)

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