Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues

ZHENG Nen-zhu; ZHANG Lin-li; LI Li; XIN Qing-wu; MIAO Zhong-wei; ZHU Zhi-ming; HUANG Yi-fan

doi:10.19303/j.issn.1008-0384.2019.08.010

Volume 34 Issue 8

Aug. 2019

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(8): 933-938

ZHENG Nen-zhu, ZHANG Lin-li, LI Li, XIN Qing-wu, MIAO Zhong-wei, ZHU Zhi-ming, HUANG Yi-fan. Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010

Citation:

ZHENG Nen-zhu, ZHANG Lin-li, LI Li, XIN Qing-wu, MIAO Zhong-wei, ZHU Zhi-ming, HUANG Yi-fan. Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010

Citation:

ZHENG Nen-zhu, ZHANG Lin-li, LI Li, XIN Qing-wu, MIAO Zhong-wei, ZHU Zhi-ming, HUANG Yi-fan. Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues[J]. Fujian Journal of Agricultural Sciences, 2019, 34(8): 933-938. doi: 10.19303/j.issn.1008-0384.2019.08.010

PDF( 1762 KB)

Expression of Cu/Zn-SOD Gene and Activity of Cu/Zn-SOD Enzyme in Mule Duck Tissues

doi: 10.19303/j.issn.1008-0384.2019.08.010

1.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2.
Institute of Animal Science and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2019-07-10
Rev Recd Date: 2019-08-03
Publish Date: 2019-08-01

Abstract

Abstract

Objective Expressions of Cu/Zn-SOD gene and activities of the antioxidant enzyme in various organs of mule ducks were determined. Method The gene expression in the heart, liver, spleen, lung, kidney, gizzard and chest muscles, antioxidant enzyme activities, and MDA contents of mule duck were determined by the qRT-PCR, xanthine-xanthine oxidase, and thiobarbituric acid methods. Result The greatest mRNA expression of Cu/Zn-SOD gene was found in the chest muscle followed by the tissues in the order of liver > lung > heart > spleen > gizzard/kidney of the ducks. The activities of Cu/Zn-SOD enzyme in the liver and kidney were significantly higher than those in the breasts and gizzard. The higher MDA content in the breasts than in the liver, gizzard or kidney indicated a greater susceptibility of the chest muscle to damage by oxidative stress. There was no significant correlation observed between the antioxidant enzyme activity and the mRNA expression level in different organs. However, the Cu/Zn-SOD enzyme activity was inversely correlated to MDA content in the tissues. Conclusion The Cu/Zn-SOD mRNA expression and enzyme activity were organ-specific. The differences in the enzymatic activities might stem from the diverse physiological functions of the enzymes and/or the tissue metabolism per se.
- Cu/Zn-SOD,
- mRNA expression,
- enzyme activity,
- mule duck

FullText(HTML)

References(25)

References

[1]	HÜPPI P S. The role of oxygen in health and disease-a series of reviews[J]. Pediatric Research, 2009, 65(3):261-268. doi: 10.1203/PDR.0b013e31818fc83f
[2]	FELLENBERG M, SPEISKY H. Antioxidants:their effects on broiler oxidative stress and its meat oxidative stability[J]. World's Poultry Science Journal, 2006, 62(1):53-70. doi: 10.1079/WPS200584
[3]	SURAI P F. Antioxidant systems in poultry biology:superoxide dismutase[J]. Journal of Animal Research and Nutrition, 2016, 1(1):8. http://cn.bing.com/academic/profile?id=c44897f900bcb020fd63c382a41c15fc&encoded=0&v=paper_preview&mkt=zh-cn
[4]	SURAI P. The relevance of the antioxidant system to the health and growth of the developing chick[J]. Australian Poultry Science Symposium, 2001:126-134. http://cn.bing.com/academic/profile?id=838063912ff4ca85bcf0f884467d9102&encoded=0&v=paper_preview&mkt=zh-cn
[5]	郑明学, 韩克光, 高建广.自由基在鸡肾病发生中的作用研究[J].农业生物技术学报, 2000, 8(2):176. doi: 10.3969/j.issn.1674-7968.2000.02.026 ZHENG M X, HAN K G, GAO J G. Effects of Free Radical in Pathogenesis of NePhrosis in Chicken[J]. Journal of Agricultural Biotechnology, 2000, 8(2):176.(in Chinese) doi: 10.3969/j.issn.1674-7968.2000.02.026
[6]	XIONG W, CHEN Y, WANG Y, et al. Roles of the antioxidant properties of icariin and its phosphorylated derivative in the protection against duck virus hepatitis[J]. BMC Veterinary Research, 2014, 10(1):226. doi: 10.1186/s12917-014-0226-3
[7]	HALLIWELL B. Free radicals and antioxidants:updating a personal view[J]. Nutrition Reviews, 2012, 70(5):257-265. doi: 10.1111/j.1753-4887.2012.00476.x
[8]	FANTEL A G. Reactive oxygen species in developmental toxicity:review and hypothesis[J]. Teratology, 1996, 53(3):196-217. doi: 10.1002/(SICI)1096-9926(199603)53:3<196::AID-TERA7>3.0.CO;2-2
[9]	JAESCHKE H. Mechanisms of oxidant stress-induced acute tissue injury[J]. Proceedings of the Society for Experimental Biology and Medicine, 1995, 209(2):104-111. doi: 10.3181/00379727-209-43885b
[10]	GÜLŞ, BELGE-KURUTAŞ E, YILDIZ E, et al. Pollution correlated modifications of liver antioxidant systems and histopathology of fish (Cyprinidae) living in Seyhan Dam Lake, Turkey[J]. Environment International, 2004, 30(5):605-609. doi: 10.1016/S0160-4120(03)00059-X
[11]	TAPPEL M E, CHAUDIERE J, TAPPEL A L. Glutathione peroxidase activities of animal tissues[J]. Comparative Biochemistry and Physiology B, Comparative Biochemistry, 1982, 73(4):945-949. doi: 10.1016/0305-0491(82)90341-8
[12]	DAUN C, ÅKESSON B. Glutathione peroxidase activity, and content of total and soluble selenium in five bovine and porcine organs used in meat production[J]. Meat Science, 2004, 66(4):801-807. doi: 10.1016/S0309-1740(03)00178-5
[13]	SURAI P F, SPEAKE B K, NOBLE R C, et al. Tissue-specific antioxidant profiles and susceptibility to lipid peroxidation of the newly hatched chick[J]. Biological Trace Element Research, 1999, 68(1):63-78. doi: 10.1007/BF02784397
[14]	孙淑霞, 倪玉平, 张连江, 等.半番鸭的养殖现状及发展前景[J].畜牧与饲料科学, 2015(10):113-115. doi: 10.3969/j.issn.1672-5190.2015.10.043 SUN S X, NI Y P, ZHANG L J, et al. Current situation and developing prospect of of mule duck[J]. Animal Husbandry and Feed Science, 2015(10):113-115.(in Chinese) doi: 10.3969/j.issn.1672-5190.2015.10.043
[15]	郑嫩珠, 李丽, 辛清武, 等.酵母硒, 黄芪多糖及其复合剂对半番鸭生长性能、肉品质和抗氧化能力的影响[J].动物营养学报, 2016, 28(12):3856-3866. http://d.old.wanfangdata.com.cn/Periodical/dwyyxb201612018 ZHENG N Z, LI L, XIN Q W, et al. Effects of selenium yeast, Astragalus polysaccharide and their compound on growth performance, meat quality and antioxidant ability of mule ducks[J]. Chinese Journal of Animal Nutrition, 2016, 28(12):3856-3866.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/dwyyxb201612018
[16]	李丽, 缪中纬, 辛清武, 等.酵母硒和黄芪多糖对半番鸭屠宰性能, 血清生化及抗氧化指标的影响[J].浙江大学学报(农业与生命科学版), 2016, 43(4):502-510. http://d.old.wanfangdata.com.cn/Periodical/zjdxxb-nyysm201704014 LI L, MIAO Z W, XIN Q W, et al. Effects of selenium-enriched yeast and astragalus polysaccharide on slaughter performance, serum biochemical and antioxidant indexes of mule duck[J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2016, 43(4):502-510.(in Chinese) http://d.old.wanfangdata.com.cn/Periodical/zjdxxb-nyysm201704014
[17]	BAEK I J, SEO D S, YON J M, et al. Tissue expression and cellular localization of phospholipid hydroperoxide glutathione peroxidase (PHG-Px) mRNA in male mice[J]. Journal of Molecular Histology, 2007, 38(3):237-244. doi: 10.1007/s10735-007-9092-7
[18]	RHEE S G. Redox signaling:hydrogen peroxide as intracellular messenger[J]. Experimental & molecular medicine, 1999, 31(2):53. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_2935341
[19]	XU C, WANG Y, GUO J, et al. Comparison of age-related differences in expression of antioxidant enzyme mRNA and activity in various tissues of pigs[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 2007, 147(3):445-451. doi: 10.1016/j.cbpb.2007.02.007
[20]	YUAN H T, BINGLE C D, KELLY F J. Differential patterns of antioxidant enzyme mRNA expression in guinea pig lung and liver during development[J]. Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1996, 1305(3):163-171. doi: 10.1016/0167-4781(95)00214-6
[21]	CULOTTA V C, YANG M, O'HALLORAN T V. Activation of superoxide dismutases:putting the metal to the pedal[J]. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2006, 1763(7):747-758. doi: 10.1016/j.bbamcr.2006.05.003
[22]	朱子玉, 曹广力, 朱越雄, 等.丝毛乌骨鸡和北京油鸡超氧化物岐化酶活性比较研究[J].中国畜牧杂志, 2000, 36(6):3-4. doi: 10.3969/j.issn.0258-7033.2000.06.001 ZHU Z Y, CAO G L, ZHU Y X, et al. Coimparison of superoxide dismutase activity in silkies and beijing fatty chickens[J]. Chinese Journal of Animal Science, 2000, 36(6):3-4.(in Chinese) doi: 10.3969/j.issn.0258-7033.2000.06.001
[23]	MARKLUND S L. Extracellular superoxide dismutase and other superoxide dismutase isoenzymes in tissues from nine mammalian species[J]. Biochemical Journal, 1984, 222(3):649-655. doi: 10.1042/bj2220649
[24]	LEI X G, ROSS D A, RONEKER K R. Comparison of age-related differences in expression of phospholipid hydroperoxide glutathione peroxidase mRNA and activity in various tissues of pigs[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology, 1997, 117(1):109-114. doi: 10.1016/S0305-0491(96)00316-1
[25]	ZHANG W, JIANG Y, ZHU Q, et al. Sodium butyrate maintains growth performance by regulating the immune response in broiler chickens[J]. British Poultry Science, 2011, 52(3):292-301. doi: 10.1080/00071668.2011.578121