Chemistry and <i>In Vitro</i> Immunological Activity of Polysaccharides from <i>Sparassis latifolia</i>

ZHANG Di; WANG Hong-yu; XIAO Dong-lai; LIN Yan-quan

doi:10.19303/j.issn.1008-0384.2019.09.015

Volume 34 Issue 9

Sep. 2019

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Article Navigation > Fujian Journal of Agricultural Sciences > 2019 > 34(9): 1093-1099

ZHANG D, WANG H Y, XIAO D L, et al. Chemistry and In Vitro Immunological Activity of Polysaccharides from Sparassis latifolia [J]. Fujian Journal of Agricultural Sciences，2019，34（9）：1093−1099. doi: 10.19303/j.issn.1008-0384.2019.09.015

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ZHANG D, WANG H Y, XIAO D L, et al. Chemistry and In Vitro Immunological Activity of Polysaccharides from Sparassis latifolia [J]. Fujian Journal of Agricultural Sciences，2019，34（9）：1093−1099. doi: 10.19303/j.issn.1008-0384.2019.09.015

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Chemistry and In Vitro Immunological Activity of Polysaccharides from Sparassis latifolia

doi: 10.19303/j.issn.1008-0384.2019.09.015

Institute of Edible & Medicinal Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350014, China

Received Date: 2019-04-19
Rev Recd Date: 2019-09-09
Publish Date: 2019-09-01

Abstract

Abstract

Objective Chemical structure and in vitro immunological activity of the water soluble polysaccharides from Sparassis latifolia were studied . Method The lyophilized powder of S. latifolia fruiting bodies was extracted by hot water to obtain polysaccharides SCG-D. From the extract, the SCG-N and SCG-A fractions were isolated by the DEAE-Sepharose Fast Flow ion exchange chromatography. The structure of SCG-A was analyzed using HPSEC, monosaccharide composition analysis, FT-IR, and NMR. The in vitro immunological activities of the extract and the fractions were determined by a test on rat spleen lymphocytes. Result SCG-D was found active in promoting the lymphocyte proliferation. SCG-A exhibited the strongest activity with a proliferation rate of 32.7% at the concentration of 100 μg/mL in 72h, while SCG-N activity was lower at 11.66%. The molecular weight of SCG-A was 4.30×10⁵Da with an α-1,4-D-glucan as its main chain consisting primarily of glucose and galactose with some 1-6-branches. Conclusion The water-soluble polysaccharides and its fractions obtained from the lyophilized S. latifolia powder showed varying in vitro immune activities of promoting the proliferation of rat spleen lymphocytes. The acidic SCG-A had a main chain structure of α-1,4-glucan with 1-6-branches and showed the greatest immunological activity among the 3 polysaccharide materials.
- Sparassis latifolia,
- polysaccharide,
- spleen lymphocyte,
- immunity

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References

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