Proteomes of Sheep Alveolar and Mouse Macrophages Infected by <i>Mycoplasma ovipneumoniae</i>

ZHANG Ying; ZHANG Kai; MA Jincheng; LI Xiaojie; MA Chunji; GAO Liyang

doi:10.19303/j.issn.1008-0384.2020.11.010

Volume 35 Issue 11

Nov. 2020

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Article Navigation > Fujian Journal of Agricultural Sciences > 2020 > 35(11): 1244-1251

ZHANG Y, ZHANG K, MA J, et al. Proteomes of Sheep Alveolar and Mouse Macrophages Infected by Mycoplasma ovipneumoniae [J]. Fujian Journal of Agricultural Sciences，2020，35（11）：1244−1251 doi: 10.19303/j.issn.1008-0384.2020.11.010

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ZHANG Y, ZHANG K, MA J, et al. Proteomes of Sheep Alveolar and Mouse Macrophages Infected by Mycoplasma ovipneumoniae [J]. Fujian Journal of Agricultural Sciences，2020，35（11）：1244−1251 doi: 10.19303/j.issn.1008-0384.2020.11.010

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Proteomes of Sheep Alveolar and Mouse Macrophages Infected by Mycoplasma ovipneumoniae

doi: 10.19303/j.issn.1008-0384.2020.11.010

1.
School of Science, Ningxia University/Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in Western China
2.
Xinhua College, Ningxia University, Yinchuan, Ningxia　750021, China

Received Date: 2020-06-12
Rev Recd Date: 2020-09-04

Available Online: 2020-11-24

Publish Date: 2020-11-30

Abstract

Abstract

Objective Feasibility of using mouse macrophage cell line Raw 264.7 for studies on pathogenesis of Mycoplasma ovipneumoniae(Mo) on sheep was investigated by comparing the proteome changes taken place in both macrophages after an induced Mo infection. Method The primary alveolar macrophages from sheep lung tissue were isolated and used, along with Raw 264.7, for the study. Both macrophages were infected with a standard strain of Mo (MOI=10) for 24h prior to proteomic and RT-PCR analyses to compare the expressions on the target genes in them. Result The image of specific surface antigen CD14 shown on the cells obtained from the lung tissue confirmed the identity of the isolated sheep primary alveolar macrophages. After infected by Mo, both sheep and mouse macrophages showed a similar significant change pattern on the expressions of FADD, IL-1β, NOS2, and THBS1. These proteins are known to associated with the Toll-like receptor signaling pathway, MAPK signaling pathway, and autophagy in biological processes. Conclusion It appeared that Raw 264.7 could be satisfactorily used to substitute the sheep primary alveolar macrophage in a simulated system for studies on Mo pathogenesis to considerably simplify the process and save cost in conducting the experiments on sheep.
- Mycoplasma ovipneumoniae,
- sheep alveolar macrophages,
- Raw 264.7,
- cell model

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References(20)

References

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