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Volume 35 Issue 12
Dec.  2020
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Article Contents
LI Y, LIU H H, LIU C J, et al. Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from Geranium wilfordii [J]. Fujian Journal of Agricultural Sciences,2020,35(12):1397−1404 doi: 10.19303/j.issn.1008-0384.2020.12.014
Citation: LI Y, LIU H H, LIU C J, et al. Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from Geranium wilfordii [J]. Fujian Journal of Agricultural Sciences,2020,35(12):1397−1404 doi: 10.19303/j.issn.1008-0384.2020.12.014

Volatiles, Flavonoids, and Antibacterial Activity of Ethanol-extract from Geranium wilfordii

doi: 10.19303/j.issn.1008-0384.2020.12.014
  • Received Date: 2020-03-06
  • Rev Recd Date: 2020-08-28
  • Publish Date: 2020-12-31
  •   Objective  Antibacterial activity and mechanism of the ethanol-extract of Geranium wilfordii Maxim on clinical pathogens were studied for development of a natural antibiotic.   Method  The chemical compositions of the extract were analyzed by GC-MS and HPC. The filter paper method was used to test the antimicrobial activity of the extract against 12 clinical samples of the resistant bacteria.   Result  GC-MS detected 18 volatile substances, including aliphatic hydrocarbons, fatty acids, and alkaloids (1.15%). The 3 flavonoid compounds identified were myricitrin, quercetin-3-O-β-D-glucose, and taxifolin. High antibacterial activities against Enterococcus faecalis ATCC 29212 (MIC 1.0 mg·mL−1), methicillin-resistant Staphylococcus aureus (MIC 1.0 mg·mL−1), Escherichia coli ATCC 25922 (MIC 1.0 mg·mL−1), and Acinetobacter baumannii ATCC 29212 (MIC 2.0 mg·mL−1) of the extract were observed.   Conclusion  In the G. wilfordii ethanol-extract, aliphatic hydrocarbons, fatty acids, and alkaloids were the major volatiles, and myricitrin, quercetin-3-O-β-D-glucose, and taxifolin the 3 substantial flavonoid compounds found. The extract demonstrated a significant antibacterial activity with wide spectrum against the commonly known clinical pathogens.
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