Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of <i>Pleurotus eryngii</i> 528

TANG Baosha; WU Li; WENG Minjie; LAI Pufu; LI Yibin

doi:10.19303/j.issn.1008-0384.2021.06.016

Volume 36 Issue 6

Jun. 2021

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TANG B S, WU L, WENG M J, et al. Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528 [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016

Citation:

TANG B S, WU L, WENG M J, et al. Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528 [J]. Fujian Journal of Agricultural Sciences，2021，36（6）：728−734 doi: 10.19303/j.issn.1008-0384.2021.06.016

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Optimization of medium for biosynthesis of ergothioneine by submerged fermentation of Pleurotus eryngii 528

doi: 10.19303/j.issn.1008-0384.2021.06.016

TANG Baosha^{1, 2
,},
WU Li^{1, 2},
WENG Minjie^{1, 2},
LAI Pufu^{1, 2},
LI Yibin^{1, 2
,
,}

1.
Institute of Agri-engineering Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China
2.
Fujian Key Laboratory of Agricultural Product (Food) Processing, Fuzhou, Fujian　350003, China

Received Date: 2020-05-20
Rev Recd Date: 2021-06-23
Publish Date: 2021-06-28

Abstract

Abstract

Objective In order to increase the ergothioneine content of Pleurotus eryngii 528 fermented products, the nutritional formula of Pleurotus eryngii 528 was screened to provide a theoretical basis for the development of high content of ergothioneine in submerged fermentation of Pleurotus eryngii . Method The suitable carbon and nitrogen sources for Pleurotus eryngii 528 mycelium growth were discussed based on the solid culture mycelium growth rate and mycelium morphology. A single factor test was conducted to study the nutritional formula of mycelium growth in Pleurotus eryngii 528 submerged fermentation, with the indexes of the dry weight of the fermented product, the sedimentation and the initial pH value of the medium. The effects of different amino acids on ergothioneine content in submerged fermentation broth were investigated. Finally, L₉(3⁴) orthogonal test was used to analyze the formula of submerged fermentation medium for increasing ergothioneine content in Pleurotus eryngii 528. Result Corn grits and peptone were the suitable carbon and nitrogen sources for the growth of Pleurotus eryngii 528. In the liquid fermentation of Pleurotus eryngii 528, the dry weight of the fermentation product in the liquid culture medium of corn grits powder 2.0% and peptone 2.0% reached 33.30±0.81 g·L⁻¹ , which was significantly higher than that of other carbon and nitrogen source combinations, and the sediment condition and initial pH value were more suitable for Pleurotus eryngii 528 liquid culture. Aspartic acid, cysteine, histidine, arginine and glutamic acid improved significantly the ergothioneine yield of Pleurotus eryngii 528 fermented product. The content of ergothioneine in the liquid fermentation of Pleurotus eryngii induced by histidine was the highest, which was 3.73 times of the control group, and was significantly higher than other amino acids. Conclusion The optimum medium formula of ergothioneine biosynthesis by submerged fermentation of Pleurotus eryngii 528 was as follows: corn grits 2.0%, peptone 1.5%, histidine 0.15%, wheat bran powder 1.0%, KH₂PO₄0.1%, MgSO₄0.1%, glutamic acid 0.1% and vitamin B6 0.1%. Under these conditions, the content of ergothioneine in Pleurotus eryngii fermentation reached 20.50±1.80 mg·L⁻¹.
- Pleurotus eryngii 528,
- liquid fermentation,
- ergothioneine,
- histidine

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References(22)

References

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