Transcriptome Changes of <i>Citrus grandis</i> Seedlings in Response to Acid Rain Stress

ZHANG Qiong; LU Luanmei; ZHU Lixia

doi:10.19303/j.issn.1008-0384.2021.07.003

Volume 36 Issue 7

Jul. 2021

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(7): 750-758

ZHANG Q, LU L M, ZHU L X. Transcriptome Changes of Citrus grandis Seedlings in Response to Acid Rain Stress [J]. Fujian Journal of Agricultural Sciences，2021，36（7）：750−758 doi: 10.19303/j.issn.1008-0384.2021.07.003

Citation:

ZHANG Q, LU L M, ZHU L X. Transcriptome Changes of Citrus grandis Seedlings in Response to Acid Rain Stress [J]. Fujian Journal of Agricultural Sciences，2021，36（7）：750−758 doi: 10.19303/j.issn.1008-0384.2021.07.003

Citation:

PDF( 893 KB)

Transcriptome Changes of Citrus grandis Seedlings in Response to Acid Rain Stress

doi: 10.19303/j.issn.1008-0384.2021.07.003

ZHANG Qiong^{1, 2
,},
LU Luanmei^{1, 2},
ZHU Lixia^{1, 2}

1.
School of Biological Sciences and Biotechnology, Zhangzhou, Fujian　363000, China
2.
Minnan Normal University, Zhangzhou, Fujian　363000, China

Received Date: 2021-03-07
Rev Recd Date: 2021-04-15

Available Online: 2021-07-13

Publish Date: 2021-07-28

Abstract

Abstract

Objective Molecular mechanisms of Citrus grandis (L.) Osbeck. cv. Guanximiyou in response to simulated acid rain stress were investigated. Method The llumina HiSeq 4000 system, a high-throughput transcriptome sequencing technology, was applied to reveal the differential expressions of the grapefruit transcriptome after a 24 h simulated acid rain treatment. The unigenes obtained were compared to the Nr and KEGG database. Abundance of gene expression of the samples were screened according to transcriptome data by using PRKM method. Differentially expressed genes (DEGs) among the treated samples were estimated by referring to the standard of FDR ≤ 0.05 and |log₂FC| ≥ 1. Functions and pathways of those DEGs were analyzed using the Gene Ontology (GO) and KEGG pathway database. Result Significant lumpy lesions began to appear on the young grapefruit leaves 24 h after the artificial acid rain spray with 21 497 fully described unigenes obtained. In comparison to control, 879 of the genes were significantly upregulated and 588 downregulated. The top 50 DEGs were all in the upregulated category and mainly associated with metabolic pathways, secondary metabolism, phenylpropyl propane biosynthesis, or terpenoid biosynthesis. The GO enrichment analysis showed the DEGs being largely located in extracellular region and, among various molecular functions, the catalytic activity being the most significantly enriched, followed by oxidoreductase activity, while the DNA metabolism being the most significant of DEGs in the GO term on biological process. The KEGG enrichment analysis indicated that DNA replication was the most significant enrichment pathway, followed by secondary metabolic biosynthesis, and lignin synthesis. The PCR fluorescence quantitative analysis on the 4 DEGs in the secondary metabolic biosynthesis pathway [i.e., POD isozyme cg3g018770 and cg2g001440, cinnamyl coenzyme A reductase (CCR) isozyme cg1g021310, and 4-coumaric acid-coenzyme A ligase (4CL) isozyme cg3g029290] confirmed that the acid rain stress indeed significantly affected the expressions of these genes. Conclusion C. grandis (L.) Osbeck. cv. Guanximiyou was strongly tolerant to acid rain. The stress response of the plants involved numerous genes regulated by various collaborative biological processes. Among them, the regulation of secondary metabolism appeared to play a major role in coping with acid rain stress by the plant.
- Citrus grandis (L.) Osbeck. cv. Guanximiyou,
- acid rain stress,
- transcriptome sequencing,
- differentially expressed genes

FullText(HTML)

References(19)

References

[1]	林燕金, 林旗华, 姜翠翠, 等. 福建省柚类产业发展现状及对策 [J]. 东南园艺, 2014, 2(5):39−42. doi: 10.3969/j.issn.1004-6089.2014.05.009 LIN Y J, LIN Q H, JIANG C C, et al. Industry status and countermeasures of pomelo industry in Fujian Province [J]. Southeast Horticulture, 2014, 2(5): 39−42.(in Chinese) doi: 10.3969/j.issn.1004-6089.2014.05.009
[2]	王梨嬛, 潘永娟, 杨莉, 等. ‘琯溪蜜柚’荧光定量PCR内参基因的筛选 [J]. 果树学报, 2013, 30(1):48−54. WANG L H, PAN Y J, YANG L, et al. Validation of internal reference genes for qRT-PCR normalization in Guanxi Sweet Pummelo(Citrus grandis) [J]. Journal of Fruit Science, 2013, 30(1): 48−54.(in Chinese)
[3]	赵卫红. 福建省主要城市降水离子特征及沉降量现状分析 [J]. 亚热带资源与环境学报, 2008, 3(3):19−24. doi: 10.3969/j.issn.1673-7105.2008.03.004 ZHAO W H. On characteristics of precipitation ion and current sedimentation situation in main cities of Fujian Province [J]. Journal of Subtropical Resources and Environment, 2008, 3(3): 19−24.(in Chinese) doi: 10.3969/j.issn.1673-7105.2008.03.004
[4]	钱笑杰, 林晓兰, 肖靖, 等. 福建果园土壤pH值、养分关系与土壤肥力质量评价研究: 以福建省漳州市平和县琯溪蜜柚园地为例 [J]. 福建热作科技, 2017, 42(1):9−15. doi: 10.3969/j.issn.1006-2327.2017.01.003 QIAN X J, LIN X L, XIAO J, et al. Evaluation research between soil pH, nutrition and soil fertility in Fujian orchard [J]. Fujian Science & Technology of Tropical Crops, 2017, 42(1): 9−15.(in Chinese) doi: 10.3969/j.issn.1006-2327.2017.01.003
[5]	任晓巧, 章家恩, 向慧敏, 等. 酸雨对植物地上部生理生态的影响研究进展与展望 [J]. 应用与环境生物学报, 2021, 27(5):1−10. REN X Q, ZHANG J E, XIANG H M, et al. Research advances and prospects for effects of acid rain on the aboveground physiology of plants and the related alleviation countermeasures [J]. Chinese Journal of Applied & Environmental Biology, 2021, 27(5): 1−10.(in Chinese)
[6]	HU W J, CHEN J, LIU T W, et al. Proteome and calcium-related gene expression in Pinus massoniana needles in response to acid rain under different calcium levels [J]. Plant and Soil, 2014, 380: 285−303. doi: 10.1007/s11104-014-2086-9
[7]	宋晓梅, 曹向阳. 模拟酸雨对不同园林植物叶片生理生态特性的影响 [J]. 水土保持研究, 2017, 24(2):365−370. SONG X M, CAO X Y. Effect of simulated acid rain on the physiological and ecological characteristics of different garden plants [J]. Research of Soil and Water Conservation, 2017, 24(2): 365−370.(in Chinese)
[8]	YAN P, WU L Q, WANG D H, et al. Soil acidification in Chinese tea plantations [J]. Science of the Total Environment, 2020, 715: 1−7.
[9]	LIU T W, NIU L, FU B, et al. A transcriptomic study reveals differentially expressed genes and pathways respond to simulated acid rain in Arabidopsis thaliana [J]. Genome, 2013, 56(1): 49−60. doi: 10.1139/gen-2012-0090
[10]	俞翰炳. 模拟酸雨对拟南芥抗病性的影响及机理探究[D]. 杭州: 杭州师范大学, 2015. YU H B. Studies on effects and mechanisms of simulated acid rain on Arabidopsis thaliana tolerance to disease[D]. Hangzhou: Hangzhou Normal University, 2015. (in Chinese).
[11]	牛力. 模拟酸雨对拟南芥某些生理特性和基因表达谱的影响[D]. 厦门: 厦门大学, 2009. NIU L. Effects of simulated acid rain on some physiological characteristics and gene expression profiles of Arabidopsis thaliana[D]. Xiamen, China: Xiamen University, 2009. (in Chinese).
[12]	张琼, 陆銮眉, 戴清霞, 等. 模拟酸雨对‘琯溪蜜柚’叶片抗氧化酶活性和光合作用的影响 [J]. 果树学报, 2018, 35(7):828−835. ZHANG Q, LU L M, DAI Q X, et al. Effects of acid rain on the leaves antioxidase activity and photosynthesis of Citrus Grandis(L.) Osbeck. ‘Guanximiyou’ seedlings [J]. Journal of Fruit Science, 2018, 35(7): 828−835.(in Chinese)
[13]	KIM D, PERTEA G, TRAPNELL C, et al. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions [J]. Genome Biology, 2013, 14(4): R36. doi: 10.1186/gb-2013-14-4-r36
[14]	TRAPNELL C, WILLIAMS B A, PERTEA G, et al. Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation [J]. Nature Biotechnology, 2010, 28(5): 511−515. doi: 10.1038/nbt.1621
[15]	任晓巧, 章家恩, 向慧敏, 等. 酸雨对植物地上部生理生态的影响研究进展与展望[J/OL]. 应用与环境生物学报: 1-12 [2021-04-25]. https://doi.org/10.19675/j.cnki.1006-687x.2020.07054. REN X Q, ZHANG J, XIANG H M, et al. Research advances and prospects for effects of acid rain on the aboveground physiology of plants and the related alleviation countermeasures [J/OL]. Chinese Journal of Applied and Environmental Biology: 1−12 [2021-04-25]. https://doi.org/10.19675/j.cnki.1006-687x.2020.07054. (in Chinese).
[16]	张利霞, 郝国宝, 常青山, 等. 模拟酸雨胁迫对夏枯草抗氧化酶活性和光合参数的影响 [J]. 中国草地学报, 2020, 42(4):56−61. ZHANG L X, HAO G B, CHANG Q S, et al. Antioxidant capacity and photosynthetic characteristics of Prunella vulgaris seedlings in response to simulated acid rain stress [J]. Chinese Journal of Grassland, 2020, 42(4): 56−61.(in Chinese)
[17]	马永佳, 梁婵娟. 外源钙对模拟酸雨胁迫下水稻质膜组分和钙形态的调节 [J]. 农业环境科学学报, 2021, 40(6): 1159-1166. MA Y J, LIANG C J. Regulation of exogenous calcium on plasma membrane compositions and calcium forms of rice roots under simulated acid rain stress [J]. Journal of Agro-Environment Science, 2021, 40(6): 1159−1166. https://kns.cnki.net/kcms/detail/12.1347.
[18]	刘洪博, 刘新龙, 苏火生, 等. 干旱胁迫下割手密根系转录组差异表达分析 [J]. 中国农业科学, 2017, 50(6):1167−1178. doi: 10.3864/j.issn.0578-1752.2017.06.017 LIU H B, LIU X L, SU H S, et al. Transcriptome difference analysis of Saccharum spontaneum roots in response to drought stress [J]. Scientia Agricultura Sinica, 2017, 50(6): 1167−1178.(in Chinese) doi: 10.3864/j.issn.0578-1752.2017.06.017
[19]	阎秀峰. 植物次生代谢生态学 [J]. 植物生态学报, 2001, 25(5):639−640, 622. doi: 10.3321/j.issn:1005-264X.2001.05.021 YAN X F. Ecology of plant secondary metabolism [J]. Acta Phytoecologica Sinica, 2001, 25(5): 639−640, 622.(in Chinese) doi: 10.3321/j.issn:1005-264X.2001.05.021