High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus

LI Yuanyuan; ZHANG Qiong; CHEN Xiaohuang; ZHANG Jinghu; LU Luanmei

doi:10.19303/j.issn.1008-0384.2021.08.012

Volume 36 Issue 8

Aug. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(8): 948-955

LI Y Y, ZHANG Q, CHEN X H, et al. High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus [J]. Fujian Journal of Agricultural Sciences，2021，36（8）：948−955 doi: 10.19303/j.issn.1008-0384.2021.08.012

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LI Y Y, ZHANG Q, CHEN X H, et al. High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus [J]. Fujian Journal of Agricultural Sciences，2021，36（8）：948−955 doi: 10.19303/j.issn.1008-0384.2021.08.012

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High-throughput Sequencing on Microbial Community in Rhizosphere Soil of Chinese Narcissus

doi: 10.19303/j.issn.1008-0384.2021.08.012

1.
Provincial Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics/College of the Bioscience and Technology, Minnan Normal University, Zhangzhou, Fujian　363000, China
2.
College of the Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, Fujian　363000, China

Received Date: 2021-03-28
Rev Recd Date: 2021-05-21
Publish Date: 2021-08-28

Abstract

Abstract

Objective The microbial community in rhizosphere soils of Chinese narcissuses was analyzed. Method The Illumina Miseq high-throughput sequencing technology was used to obtain the relevant conserved gene regions of the microbes in soil specimens from Chinese narcissus planting lots. The community structures and distributions of dominant species of bacteria, fungi, and archaea in the rhizosphere soils were analyzed. Result In total, 175 840 optimized sequences were obtained and clustered from the specimens into 2 680 representative OTUs with a 97% similarity. The dominant bacteria were Chloroflexi (30.86%) and Proteobacteria (20.67%). Among the fungi, Ascomycota (84.94%) significantly overshadowed the others, with Chaetomium globosum (28.15%) and Ascomycetes (25.01%) accounted for the greater proportions. On archaea, Thaumarchaeota (51.40%), Bathyarchaeota (25.98%), and Euryarchaeota (20.65%) were the major phyla that had 25.67% SCG and 12.93% acidophilic ammonia oxidizing members. Conclusion The rhizosphere soils of Chinese narcissuses harbored diverse and rich microbial species. The information obtained would aid the development and utilization of the natural resources.
- Chinese narcissuses,
- rhizosphere soil microorganisms,
- high-throughput sequencing,
- bacteria,
- fungi,
- archaea

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References(49)

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