Identification and Expression Analysis of <i>ETR</i> Gene Family in <i>Camellia sinensis</i>

YAN Meihong; ZHENG Yucheng; HOU Binghao; CHEN Xuejin; CHEN Xiaojun; YE Naixing

doi:10.19303/j.issn.1008-0384.2021.10.007

Volume 36 Issue 10

Oct. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(10): 1160-1168

YAN M H, ZHENG Y C, HOU B H, et al. Identification and Expression Analysis of ETR Gene Family in Camellia sinensis [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1160−1168 doi: 10.19303/j.issn.1008-0384.2021.10.007

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YAN M H, ZHENG Y C, HOU B H, et al. Identification and Expression Analysis of ETR Gene Family in Camellia sinensis [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1160−1168 doi: 10.19303/j.issn.1008-0384.2021.10.007

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Identification and Expression Analysis of ETR Gene Family in Camellia sinensis

doi: 10.19303/j.issn.1008-0384.2021.10.007

College of Horticulture, Fujian Agriculture and Forestry University/Key Laboratory of Tea Science at University in Fujian, Fuzhou, Fujian　350002, China

Received Date: 2021-05-26
Rev Recd Date: 2021-08-30

Available Online: 2021-10-23

Publish Date: 2021-10-28

Abstract

Abstract

Objective CsETRs were identified and analyzed to predict the potential molecular functions involving the mechanism of ethylene receptor in response to stress in tea plants. Method Bioinformatics was used to identify members in the ETR family and predict their potential molecular functions. Real-time fluorescence quantitative analysis was employed for the expressions of the genes under stresses. Result Six ETRs were identified from the genome of tea plants. They all consisted of N-terminal transmembrane region, GAF region, and histidine (His) kinase domain. Phylogenetic tree analysis divided them into two groups. Each member of the ETR family contained 1-12 exons. Significantly differentiated in tissues, the expressions of ETRs were high in the fruits and stems. The fluorescence quantitative expressions of the 6 genes, especially ERS1-1, were upregulated to varying degrees when exposed to low temperature. Under the stress of plant growth regulators ABA, JA, or GA, most of the genes were upregulated, especially ERS1-3, but ETR2-2 downregulated slowly when treated by ABA. Conclusion On tea plants, 6 CsETRs in the family were identified with their potential molecular functions predicted and analyzed in this study. CsETRs were highly expressed in the fruits and stems that could be induced by low-temperature stress. Whereas ABA, MeJA, or GA could significantly upregulate the expression of ERS1-3.
- Camellia sinensis,
- ETR gene,
- plant growth regulators,
- abiotic stress,
- expression analysis

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References(34)

References

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