Identification and Growth-promoting Effect of a Phosphate-solubilizing Fungus on Wheat Seedlings

LI Jing; AI Jiamin; YU Tianfei; BIAN Dan; ZHENG Chaochao; GUO Minhao; DENG Zhenshan

doi:10.19303/j.issn.1008-0384.2021.10.015

Volume 36 Issue 10

Oct. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(10): 1224-1230

LI J, AI J M, YU T F, et al. Identification and Growth-promoting Effect of a Phosphate-solubilizing Fungus on Wheat Seedlings [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1224−1230 doi: 10.19303/j.issn.1008-0384.2021.10.015

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LI J, AI J M, YU T F, et al. Identification and Growth-promoting Effect of a Phosphate-solubilizing Fungus on Wheat Seedlings [J]. Fujian Journal of Agricultural Sciences，2021，36（10）：1224−1230 doi: 10.19303/j.issn.1008-0384.2021.10.015

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Identification and Growth-promoting Effect of a Phosphate-solubilizing Fungus on Wheat Seedlings

doi: 10.19303/j.issn.1008-0384.2021.10.015

1.
College of Life Sciences, Yan'an University, Yan’an, Shaanxi　716000, China
2.
College of Life Science, Northwest University, Xi’an, Shaanxi　710069, China

Received Date: 2021-06-21
Rev Recd Date: 2021-08-08

Available Online: 2021-10-23

Publish Date: 2021-10-28

Abstract

Abstract

Objective A fungal strain with phosphate-solubilizing and growth promoting properties was isolated from the soil of a Triticum aestivum L. field to investigate its potential as a microbial fertilizer. Methods From the soil at a wheat field, a fungal strain, R3, exhibiting a strong phosphate-solubilizing ability on an inorganic phosphorus medium was isolated. Morphological observation and ITS sequence analysis were performed for taxonomic identification on the isolate. Ability of the isolate in solubilizing Ca₃(PO₄)₂, AlPO₄, and FePO₄ was determined in a liquid culture. A pot experiment was conducted to evaluate the growth-promoting effect of the isolate on T. aestivum seedlings. Result R3 was identified as Penicillium rubens. It produced an average diameter of phosphorus dissolving ring (D) of 27.99 mm, a colony diameter (d) of 19.26 mm, and D/d ratio at 1.45 on the inorganic phosphorus medium. The solubilization on Ca₃(PO₄)₂ and AlPO₄ by R3 peaked on the 4^th day of culture at 328.79 mg·L⁻¹ and 95.99 mg·L⁻¹, respectively, while that on FePO₄ maxed on the 5^th day at 75.39 mg·L⁻¹. The pH of phosphorous solubilization medium by R3 decreased initially and then stable. The pot experiment with wheat seedlings showed the incorporation of R3 in the medium increased the height, root length, fresh weight, and chlorophyll content of the seedlings by 18.23%–35.65%, 27.63%–50.44%, 37.99%–50.94%, and 9.59%–19.57%, respectively, over control. Conclusion The phosphorus-solubilizing R3 demonstrated a significant growth promoting effect on the wheat seedlings in a pot experiment. It could be a potential candidate as a microbial agent for farm fertilization.
- Penicillium,
- phosphate-solubilizing ability,
- Triticum aestivum L.,
- growth-promoting property

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References

[1]	韦杏花. 一株解磷菌的筛选及其培养条件优化[D]. 杭州: 浙江大学, 2015. WEI X H. Screening of a phosphate dissolving bacteria strain and it's culture conditions optimization[D]. Hangzhou: Zhejiang University, 2015. (in Chinese)
[2]	KUCEY R M N, JANZEN H H, LEGGETT M E. Microbially mediated increases in plant-available phosphorus [J]. Advances in Agronomy, 1989, 42: 199−228.
[3]	林启美, 赵海英, 赵小蓉. 4株溶磷细菌和真菌溶解磷矿粉的特性 [J]. 微生物学通报, 2002, 29(6):24−28. doi: 10.3969/j.issn.0253-2654.2002.06.007 LIN Q M, ZHAO H Y, ZHAO X R. The characteristics of solubilizing rock phosphate by four isolates of bacteria and fungi [J]. Microbiology, 2002, 29(6): 24−28.(in Chinese) doi: 10.3969/j.issn.0253-2654.2002.06.007
[4]	江丽华, 刘兆辉, 石璟, 等. 真菌F2的解磷能力及其生长动态研究 [J]. 中国农学通报, 2009, 25(1):42−46. JIANG L H, LIU Z H, SHI J, et al. Solubilization capacity of insoluble phosphates and it`s growth of biomass by the phosphate fungi (F2) [J]. Chinese Agricultural Science Bulletin, 2009, 25(1): 42−46.(in Chinese)
[5]	CHUNG H, PARK M, MADHAIYAN M, et al. Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea [J]. Soil Biology and Biochemistry, 2005, 37(10): 1970−1974. doi: 10.1016/j.soilbio.2005.02.025
[6]	GHOSH R, BARMAN S, MUKHERJEE R, et al. Role of phosphate solubilizing Burkholderia spp. for successful colonization and growth promotion of Lycopodium cernuum L. (Lycopodiaceae) in lateritic belt of Birbhum district of West Bengal, India [J]. Microbiological Research, 2016, 183: 80−91. doi: 10.1016/j.micres.2015.11.011
[7]	DELVASTO P, VALVERDE A, BALLESTER A, et al. Characterization of brushite as a re-crystallization product formed during bacterial solubilization of hydroxyapatite in batch cultures [J]. Soil Biology and Biochemistry, 2006, 38(9): 2645−2654. doi: 10.1016/j.soilbio.2006.03.020
[8]	许昌超, 张俊涛, 叶少萍, 等. 土壤中一株溶磷青霉菌的分离鉴定及其应用效果研究 [J]. 中国土壤与肥料, 2020(6):272−278. doi: 10.11838/sfsc.1673-6257.19484 XU C C, ZHANG J T, YE S P, et al. Isolation and the plant growth-promoting capacity characterization of a Penicillium strain derived from soil [J]. Soil and Fertilizer Sciences in China, 2020(6): 272−278.(in Chinese) doi: 10.11838/sfsc.1673-6257.19484
[9]	朱德旋, 杜春梅, 董锡文, 等. 一株寒地高效解无机磷细菌的分离鉴定及拮抗作用 [J]. 微生物学报, 2020, 60(8):1672−1682. ZHU D X, DU C M, DONG X W, et al. Identification and antagonism activity of an inorganic phosphorus-dissolving bacterial strain isolated from cold region [J]. Acta Microbiologica Sinica, 2020, 60(8): 1672−1682.(in Chinese)
[10]	DA SILVA A V, DE OLIVEIRA A J, TANABE I S B, et al. Antarctic lichens as a source of phosphate-solubilizing bacteria [J]. Extremophiles, 2021, 25(2): 181−191. doi: 10.1007/s00792-021-01220-5
[11]	李云飞, 华陈意, 宗凯, 等. 一株分离自水稻种子的真菌的鉴定及系统进化分析 [J]. 农学学报, 2021, 11(3):36−40. doi: 10.11923/j.issn.2095-4050.cjas20190500034 LI Y F, HUA C Y, ZONG K, et al. A fungus from rice: Species identification and phylogenetic analysis [J]. Journal of Agriculture, 2021, 11(3): 36−40.(in Chinese) doi: 10.11923/j.issn.2095-4050.cjas20190500034
[12]	何迪, 耿丽平, 郭佳, 等. 草酸青霉菌HB1溶磷能力及作用机制 [J]. 农业工程学报, 2020, 36(2):255−265. doi: 10.11975/j.issn.1002-6819.2020.02.030 HE D, GENG L P, GUO J, et al. Ability and mechanism of Penicillium oxalicum HB₁ solubilizing phosphates [J]. Transactions of the Chinese Society of Agricultural Engineering, 2020, 36(2): 255−265.(in Chinese) doi: 10.11975/j.issn.1002-6819.2020.02.030
[13]	宫安东, 朱梓钰, 路亚南, 等. 吡咯伯克霍尔德菌WY6-5的溶磷、抑菌与促玉米生长作用研究 [J]. 中国农业科学, 2019, 52(9):1574−1586. doi: 10.3864/j.issn.0578-1752.2019.09.009 GONG A D, ZHU Z Y, LU Y N, et al. Functional analysis of Burkholderia pyrrocinia WY₆-5 on phosphate solubilizing, antifungal and growth-promoting activity of maize [J]. Scientia Agricultura Sinica, 2019, 52(9): 1574−1586.(in Chinese) doi: 10.3864/j.issn.0578-1752.2019.09.009
[14]	魏景超. 真菌鉴定手册[M]. 上海: 上海科学技术出版社, 1979.
[15]	张婧, 刘广娜, 左蔚琳. 土壤微生物基因组DNA不同提取方法的比较及PCR扩增体系的建立 [J]. 吉林农业, 2018(16):55−56. ZHANG J, LIU G N, ZUO W L. Comparison of different extraction methods of soil microbial genomic DNA and establishment of PCR amplification system [J]. Agriculture of Jilin, 2018(16): 55−56.(in Chinese)
[16]	吕俊, 于存. 一株高效溶磷伯克霍尔德菌的筛选鉴定及对马尾松幼苗的促生作用 [J]. 应用生态学报, 2020, 31(9):2923−2934. LYU J, YU C. Screening and identification of an efficient phosphate-solubilizing Burkholderia sp. and its growth-promoting effect on Pinus massoniana seedling [J]. Chinese Journal of Applied Ecology, 2020, 31(9): 2923−2934.(in Chinese)
[17]	张磊, 叶大柠, 朱焱, 等. 10株蜃楼弗朗西斯菌的鉴定与特征分析 [J]. 临床检验杂志, 2017, 35(4):271−276. ZHANG L, YE D N, ZHU Y, et al. Identification and characterization of 10 Francisella philomiragia strains [J]. Chinese Journal of Clinical Laboratory Science, 2017, 35(4): 271−276.(in Chinese)
[18]	许昌超, 郑富海, 李铤, 等. 土壤中一株溶磷菌的筛选和溶磷能力初探 [J]. 安徽大学学报(自然科学版), 2019, 43(5):103−108. XU C C, ZHENG F H, LI T, et al. Isolation and solubilization capacity characterization of a phosphate-solubilizing strain derived from soil [J]. Journal of Anhui University (Natural Science Edition), 2019, 43(5): 103−108.(in Chinese)
[19]	邓振山, 陈凯凯, 李静, 等. 巨菌草根部促生菌的筛选及其促生效应 [J]. 广西植物, 2020, 40(9):1323−1331. doi: 10.11931/guihaia.gxzw201904026 DENG Z S, CHEN K K, LI J, et al. Screening of growth-promoting bacteria associated with Pennisetum sinese root and their abilities of growth-promoting effect [J]. Guihaia, 2020, 40(9): 1323−1331.(in Chinese) doi: 10.11931/guihaia.gxzw201904026
[20]	张健. 低磷胁迫下草酸青霉菌BK溶磷的分子机制[D]. 大连: 大连理工大学, 2014. ZHANG J. Molecular mechanism of phosphate solubilization to phosphate deficient stress in Penicillium oxalicum BK[D]. Dalian: Dalian University of Technology, 2014. (in Chinese)
[21]	EFTHYMIOU A, GRØNLUND M, MÜLLER-STÖVER D S, et al. Augmentation of the phosphorus fertilizer value of biochar by inoculation of wheat with selected Penicillium strains [J]. Soil Biology and Biochemistry, 2018, 116: 139−147. doi: 10.1016/j.soilbio.2017.10.006
[22]	GÓMEZ-MUÑOZ B, JENSEN L S, NEERGAARD A, et al. Effects of Penicillium bilaii on maize growth are mediated by available phosphorus [J]. Plant and Soil, 2018, 431(1/2): 159−173.
[23]	赖鉴添, 杨婷, 史发超, 等. 蔗叶堆肥中一株泡盛曲霉溶磷能力的鉴定及其对辣椒的促生效果 [J]. 微生物学报, 2021, 61(1):77−91. LAI J T, YANG T, SHI F C, et al. A phosphorus-dissolving Aspergillus awamori strain from sugarcane leaf compost for growth promotion of pepper [J]. Acta Microbiologica Sinica, 2021, 61(1): 77−91.(in Chinese)
[24]	赵龙飞, 徐亚军, 曹冬建, 等. 溶磷性大豆根瘤内生菌的筛选、抗性及系统发育和促生 [J]. 生态学报, 2015, 35(13):4425−4435. ZHAO L F, XU Y J, CAO D J, et al. Screening, resistance, phylogeny and growth promoting of phosphorus solubilizing bacteria isolated from soybean root nodules [J]. Acta Ecologica Sinica, 2015, 35(13): 4425−4435.(in Chinese)
[25]	李豆豆, 尚双华, 韩巍, 等. 一株高效解磷真菌新菌株的筛选鉴定及解磷特性 [J]. 应用生态学报, 2019, 30(7):2384−2392. LI D D, SHANG S H, HAN W, et al. Screening, identification, and phosphate solubilizing characteristics of a new efficient phosphate solubilizing fungus [J]. Chinese Journal of Applied Ecology, 2019, 30(7): 2384−2392.(in Chinese)
[26]	王丹, 詹婧, 孙庆业. 出芽短梗霉F4的溶磷能力及机理 [J]. 应用生态学报, 2014, 25(7):2079−2084. WANG D, ZHAN J, SUN Q Y. Phosphate solubilization of Aureobasidium pullulan F4 and its mechanism [J]. Chinese Journal of Applied Ecology, 2014, 25(7): 2079−2084.(in Chinese)
[27]	江红梅, 殷中伟, 史发超, 等. 一株耐盐溶磷真菌的筛选、鉴定及其生物肥料的应用效果 [J]. 植物营养与肥料学报, 2018, 24(3):728−742. doi: 10.11674/zwyf.17468 JIANG H M, YIN Z W, SHI F C, et al. Isolation and functional evaluation of phosphate-solubilizing fungi with salt-tolerant characteristics [J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(3): 728−742.(in Chinese) doi: 10.11674/zwyf.17468