Transcriptome Sequencing on Eight <i>Psidium guajava</i> Cultivars

ZHANG Limei; CHEN Zhongdian; ZHANG Chaokun; WEI Xiuqing; XU Jiahui

doi:10.19303/j.issn.1008-0384.2021.11.008

Volume 36 Issue 11

Nov. 2021

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Article Navigation > Fujian Journal of Agricultural Sciences > 2021 > 36(11): 1315-1322

ZHANG L M, CHEN Z D, ZHANG C K, et al. Transcriptome Sequencing on Eight Psidium guajava Cultivars [J]. Fujian Journal of Agricultural Sciences，2021，36（11）：1315−1322 doi: 10.19303/j.issn.1008-0384.2021.11.008

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ZHANG L M, CHEN Z D, ZHANG C K, et al. Transcriptome Sequencing on Eight Psidium guajava Cultivars [J]. Fujian Journal of Agricultural Sciences，2021，36（11）：1315−1322 doi: 10.19303/j.issn.1008-0384.2021.11.008

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Transcriptome Sequencing on Eight Psidium guajava Cultivars

doi: 10.19303/j.issn.1008-0384.2021.11.008

1.
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
3.
Zhangzhou Institute of Agricultural Sciences of Fujian Province, Zhangzhou, Fujian　363000, China

Received Date: 2021-06-27
Rev Recd Date: 2021-12-08

Available Online: 2021-12-30

Publish Date: 2021-11-28

Abstract

Abstract

Objective Transcriptomes of different guava cultivars were obtained for comparison. Methods Transcriptome sequencing by Illumina Hwaseq 2 500 was conducted on 8-9 mature fruit pulp from 8 varieties of Psidium guajava Linn, namely Red Heart (HX), Rainbow (CH), Purplish Red (ZH), Crystal (SJ), Shuzhen (XZ), Colored-leaf B (CYB), Colored-leaf (CY), and Purple Star (ZX). Results By comparing to the database, 126979 Unigenes of an average length of 766 bp with a N₅₀ of 1654 bp and a N₉₀ of 267 bp were identified. There were 27274 genes co-expressed in the 8 cultivars. The PCA and differential gene expression cluster analyses showed that the differences on the function expressions between the genes of SJ and CYB was less than those between ZH and other cultivars. SJ and CYB were clustered in a category and HX, CH, XZ, CY, and ZX in a different category, while ZH significantly differentiated from the other cultivars. Conclusion The substantial amount of transcription data secured from this study could be useful for the classification as well as the expression and identification of functional genes of guava germplasms.
- Psidium guajava Linn.,
- transcriptome,
- sequencing,
- functional annotation

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References(35)

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