Diversity and Isolation of Symbiotic Bacteria on <i>Delia antiqua</i> Larvae

LIU Mei; ZHANG Xinjian; ZHOU Fangyuan

doi:10.19303/j.issn.1008-0384.2022.01.012

Volume 37 Issue 1

Jan. 2022

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(1): 84-95

LIU M, ZHANG X J, ZHOU F Y. Diversity and Isolation of Symbiotic Bacteria on Delia antiqua Larvae [J]. Fujian Journal of Agricultural Sciences，2022，37（1）：84−95 doi: 10.19303/j.issn.1008-0384.2022.01.012

Citation:

LIU M, ZHANG X J, ZHOU F Y. Diversity and Isolation of Symbiotic Bacteria on Delia antiqua Larvae [J]. Fujian Journal of Agricultural Sciences，2022，37（1）：84−95 doi: 10.19303/j.issn.1008-0384.2022.01.012

Citation:

PDF( 1397 KB)

Diversity and Isolation of Symbiotic Bacteria on Delia antiqua Larvae

doi: 10.19303/j.issn.1008-0384.2022.01.012

LIU Mei^{1, 2
,},
ZHANG Xinjian¹,
ZHOU Fangyuan^{1
,
,}

1.
Shandong Provincial Key Laboratory of Applied Microbiology/Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Ji’nan, Shandong　250013, China
2.
School of Life Sciences, Hubei University, Wuhan, Hubei　430062, China

Received Date: 2021-09-11
Rev Recd Date: 2021-11-22

Available Online: 2022-01-21

Publish Date: 2022-01-28

Abstract

Abstract

Objective Diversity of the symbiotic bacteria on Delia antiqua larvae was studied, and effectiveness of two commonly used culture media in isolating them compared. Methods Diversity of the bacterial population on the surface and in the guts of D. antiqua larvae were determined using the high-throughput amplicon sequencing technique. The effectiveness of the LB and TSB media to culture and isolate the bacteria were compared based on the sequencing results. Results The inhabitation bacteria on the larvae were from 115 genera, 66 families, and 4 phyla according to the sequencing. Most of them could not be annotated to species level. On LB medium, 463 strains were isolated that belonged to 29 species, 18 genera, and 11 families, while on TSB, 391 strains from 44 species, 28 genera, and 19 families. TSB isolated more species than LB, and some of the species could be isolated by either method. However, the high-throughput amplicon sequencing revealed that these culture methods could provide only a minute portion of the bacteria population that coinhabited on the host. Conclusion It was necessary to use both TSB and LB media in order to maximize the isolation of all symbiotic bacteria on D. antiqua larvae.
- Onion maggot,
- gut microbe,
- bacterial isolation and identifications,
- microbial diversity

FullText(HTML)

References(48)

References

[1]	NAAZ N, CHOUDHARY J S, PRABHAKAR C S, et al. Identification and evaluation of cultivable gut bacteria associated with peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) [J]. Phytoparasitica, 2016, 44(2): 165−176. doi: 10.1007/s12600-016-0518-1
[2]	LIU L J, MARTINEZ S I, MAZZON L, et al. Bacterial communities associated with invasive populations of Bactrocera dorsalis (Diptera: Tephritidae) in China [J]. Bulletin of Entomological Research, 2016, 106(6): 718−728. doi: 10.1017/S0007485316000390
[3]	LI K L, CHEN H Y, JIANG J J, et al. Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China [J]. Scientific Reports, 2016, 6: 36406. doi: 10.1038/srep36406
[4]	DOUGLAS A E. Multiorganismal insects: Diversity and function of resident microorganisms [J]. Annual Review of Entomology, 2015, 60: 17−34. doi: 10.1146/annurev-ento-010814-020822
[5]	VERAS F F, CORREA A P F, WELKE J E, et al. Inhibition of mycotoxin-producing fungi by Bacillus strains isolated from fish intestines [J]. International Journal of Food Microbiology, 2016, 238: 23−32. doi: 10.1016/j.ijfoodmicro.2016.08.035
[6]	BANSAL R, HULBERT S, SCHEMERHORN B, et al. Hessian fly-associated bacteria: transmission, essentiality, and composition [J]. Plos One, 2011, 6(8): e23170. doi: 10.1371/journal.pone.0023170
[7]	MCCUTCHEON J P, MORAN N A. Extreme genome reduction in symbiotic bacteria [J]. Nature Reviews Microbiology, 2011, 10(1): 13−26.
[8]	MORALES-JIMÉNEZ J, ZÚÑIGA G, VILLA-TANACA L, et al. Bacterial community and nitrogen fixation in the red turpentine beetle, Dendroctonus valens LeConte (Coleoptera: Curculionidae Microbial Ecology, 2009, 58(4): 879−891.
[9]	SHARON G, SEGAL D, RINGO J M, et al. Correction for bencivenni et al., direct asymmetric vinylogous michael addition of cyclic enones to nitroalkenes via dienamine catalysis [J]. Proceedings of the National Academy of Sciences, 2013, 110(12): 4852−4852.
[10]	VISÔTTO L E, OLIVEIRA M G A, GUEDES R N C, et al. Contribution of gut bacteria to digestion and development of the velvetbean caterpillar, Anticarsia gemmatalis [J]. Journal of Insect Physiology, 2009, 55(3): 185−191. doi: 10.1016/j.jinsphys.2008.10.017
[11]	ZHOU F Y, WANG Z, XUE M, et al. Toxicity of selected insecticides for onion maggot Delia antiqua (Meigen) adult and their bait-allure efficacy in the fields [J]. Journal of Food Agriculture and Environment, 2013, 11(3): 1409−1413.
[12]	THAKUR A, DHAMMI P, SAINI H S, et al. Pathogenicity of bacteria isolated from gut of Spodoptera litura (Lepidoptera: Noctuidae) and fitness costs of insect associated with consumption of bacteria [J]. Journal of Invertebrate Pathology, 2015, 127: 38−46. doi: 10.1016/j.jip.2015.02.007
[13]	XU L T, LOU Q Z, CHENG C H, et al. Gut-associated bacteria of Dendroctonus valens and their involvement in verbenone production [J]. Microbial Ecology, 2015, 70(4): 1012−1023. doi: 10.1007/s00248-015-0625-4
[14]	陈娟. 两种木食性白蚁肠道内共生细菌多样性的比较研究 [D]. 武汉: 华中师范大学, 2011. CHEN J. Comparative studies on the diversity of intestinal symbiotic bacteria between two different wood-feeding termites [D]. Wuhan: Central China Normal University, 2011.
[15]	张义强. 蜜蜂肠道共生菌的研究 [D]. 福州: 福建农林大学, 2013. ZHANG Y Q. The study of the symbiotic bacteria in honeybee intestinal tract [D]. Fuzhou: Fujian Agriculture and Forestry University, 2013.
[16]	ADAMS A S, BOONE C K, BOHLMANN J, et al. Responses of bark beetle-associated bacteria to host monoterpenes and their relationship to insect life histories [J]. Journal of Chemical Ecology, 2011, 37(8): 808−817. doi: 10.1007/s10886-011-9992-6
[17]	朱临. 淡色库蚊肠道细菌多样性分析 [D]. 淮南: 安徽理工大学, 2011. ZHU L. The analysis of diversity of bacteria of the Culex Pipiens Pallens' intestinal canal [D]. Huainan: AnHui University of Science and Technology, 2011. (in Chinese)
[18]	孙博通, 蓝波妙, 王倩, 等. 斜纹夜蛾幼虫肠道细菌分离鉴定及其功能初步分析 [J]. 生物资源, 2017, 39(4):264−271. SUN B T, LAN M B, WANG Q, et al. lsolation and preliminary functional analysis of the larval gut bacteria from Spodoptera litura [J]. Biotic Resources, 2017, 39(4): 264−271.(in Chinese)
[19]	刘玉升, 李明立, 刘俊展, 等. 东亚飞蝗肠道细菌的研究 [J]. 中国微生态学杂志, 2007, 19(1):34−36,39. doi: 10.3969/j.issn.1005-376X.2007.01.014 LIU Y S, LI M L, LIU J Z, et al. Study on the intestinal bacteria in L. migratoria manilensis [J]. Chinese Journal of Microecology, 2007, 19(1): 34−36,39.(in Chinese) doi: 10.3969/j.issn.1005-376X.2007.01.014
[20]	张云霞, 薛明, 宋增明. 葱蝇Delia-antiqua(Meigen)的研究进展 [J]. 山东农业大学学报(自然科学版), 2003, 34(3):455−458. ZHANG Y X, XUE M, SONG Z M. Progesses in Delia antiqua(Meigen) study [J]. Journal of Shandong Agricultural University (Natural Science), 2003, 34(3): 455−458.(in Chinese)
[21]	EYMANN M, FRIEND W G. Development of onion maggots (Diptera: Anthomyiidae) on bacteria-free onion agar supplemented with vitamins and amino acids [J]. Annals of the Entomological Society of America, 1985, 78(2): 182−185. doi: 10.1093/aesa/78.2.182
[22]	SCHNEIDER W D, MILLER J R, BREZNAK J A, et al. Onion maggot, Delia antiqua, survival and development on onions in the presence and absence of microorganisms [J]. Entomologia Experimentalis et Applicata, 2011, 33(1): 50−56.
[23]	SHAO Y Q, CHEN B S, SUN C, et al. Symbiont-derived antimicrobials contribute to the control of the lepidopteran gut microbiota [J]. Cell Chemical Biology, 2017, 24(1): 66−75. doi: 10.1016/j.chembiol.2016.11.015
[24]	ZHOU F Y, WU X Q, XU L T, et al. Repressed Beauveria bassiana infections in Delia antiqua due to associated microbiota [J]. Pest Management Science, 2019, 75(1): 170−179. doi: 10.1002/ps.5084
[25]	ZHANG J Y, LIU Y X, GUO X X, et al. High-throughput cultivation and identification of bacteria from the plant root microbiota [J]. Nature Protocols, 2021, 16(2): 988−1012. doi: 10.1038/s41596-020-00444-7
[26]	CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al. QIIME allows analysis of high-throughput community sequencing data [J]. Nature Methods, 2010, 7(5): 335−336. doi: 10.1038/nmeth.f.303
[27]	EDGAR R C. Search and clustering orders of magnitude faster than BLAST [J]. Bioinformatics, 2010, 26(19): 2460−2461. doi: 10.1093/bioinformatics/btq461
[28]	WANG Q, GARRITY G M, TIEDJE J M, et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy [J]. Applied and Environmental Microbiology, 2007, 73(16): 5261−5267. doi: 10.1128/AEM.00062-07
[29]	QUAST C, PRUESSE E, YILMAZ P, et al. The SILVA ribosomal RNA gene database project: Improved data processing and web-based tools[J]. Nucleic Acids Research, 2013, 41(Database issue): D590-D596.
[30]	LIU M, ZHAO X Y, LI X X, et al. Antagonistic effects of Delia antiqua (Diptera: Anthomyiidae)-associated bacteria against four phytopathogens [J]. Journal of Economic Entomology, 2021, 114(2): 597−610. doi: 10.1093/jee/toab002
[31]	王媛媛. 抗镰刀菌芽孢杆菌的分离、筛选、鉴定及其抗菌机理初步研究 [D]. 保定: 河北农业大学, 2013. WANG Y Y. Isolation, screening and identification of anti-Fusarium Bacillus and preliminary study of the disease-suppressive mechanisms. [D]. Baoding: Agriculture University of Hebei, 2013.
[32]	庞发虎. 小麦内生细菌的种群多样性调查及其在小麦条锈病生物防治中的利用研究 [D]. 南宁: 广西大学, 2016. PANG F H. Studies on the population diversity of bacterial endophytes in wheat plants and their application in the biocontrol of wheat stripe rust [D]. Nanning: Guangxi University, 2016. (in Chinese)
[33]	CUI L X, YANG C D, WEI L J, et al. Isolation and identification of an endophytic bacteria Bacillus velezensis 8-4 exhibiting biocontrol activity against potato scab [J]. Biological Control, 2020, 141: 104156. doi: 10.1016/j.biocontrol.2019.104156
[34]	刘婧, 陈丹, 庄桂芬, 等. 家蝇发育过程中肠道可培养共生细菌的分离与鉴定 [J]. 中国寄生虫学与寄生虫病杂志, 2017, 35(2):120−124. LIU J, CHEN D, ZHUANG G F, et al. Isolation and identification of cultivable symbiotic bacteria from the intestinal tract of Musca domestica during development [J]. Chinese Journal of Parasitology and Parasitic Diseases, 2017, 35(2): 120−124.(in Chinese)
[35]	LIU Y P, XU L T, ZHANG Z Q, et al. Isolation, identification, and analysis of potential functions of culturable bacteria associated with an invasive gall wasp, Leptocybe invasa [J]. Microbial Ecology, 2021: 1−16.
[36]	LUO A R, ZHANG Y Z, QIAO H J, et al. Outgroup selection in tree reconstruction: a case study of the family Halictidae (Hymenoptera: Apoidea) [J]. Acta Entomologica Sinica, 2010, 53(2): 192−201.
[37]	LYSYK T J, KALISCHUK-TYMENSEN L, SELINGER L B, et al. Rearing stable fly larvae (Diptera: Muscidae) on an egg yolk medium [J]. Journal of Medical Entomology, 1999, 36(3): 382−388. doi: 10.1093/jmedent/36.3.382
[38]	WANG S C, WANG L Y, FAN X, et al. An insight into diversity and functionalities of gut microbiota in insects [J]. Current Microbiology, 2020, 77(9): 1976−1986. doi: 10.1007/s00284-020-02084-2
[39]	ENGEL P, MORAN N A. The gut microbiota of insects - diversity in structure and function [J]. FEMS Microbiology Reviews, 2013, 37(5): 699−735. doi: 10.1111/1574-6976.12025
[40]	GHODAKE G S, KALME S D, JADHAV J P, et al. Purification and partial characterization of lignin peroxidase from Acinetobacter calcoaceticus NCIM 2890 and its application in decolorization of textile dyes [J]. Applied Biochemistry and Biotechnology, 2009, 152(1): 6−14. doi: 10.1007/s12010-008-8258-4
[41]	HU X, LI M, RAFFA K F, et al. Bacterial communities associated with the pine wilt disease vector Monochamus alternatus (Coleoptera: Cerambycidae) during different larval instars [J]. Journal of Insect Science, 2017, 17(6): 115.
[42]	GUPTA A K, RASTOGI G, NAYDUCH D, et al. Molecular phylogenetic profiling of gut-associated bacteria in larvae and adults of flesh flies [J]. Medical and Veterinary Entomology, 2014, 28(4): 345−354. doi: 10.1111/mve.12054
[43]	KSENTINI I, GHARSALLAH H, SAHNOUN M et al. Providencia entomophila sp. nov. , a new bacterial species associated with major olive pests in Tunisia [J]. PLoS One, 2019, 14(10). Doi: 10.1371/journal.pone.0223943
[44]	GUJJAR N R, GOVINDAN S, VERGHESE A, et al. Diversity of the cultivable gut bacterial communities associated with the fruit flies Bactrocera dorsalis and Bactrocera cucurbitae (Diptera: Tephritidae) [J]. Phytoparasitica, 2017, 45(4): 453−460. doi: 10.1007/s12600-017-0604-z
[45]	PANDIARAJAN J, KRISHNAN M. Comparative bacterial survey in the gut of lepidopteran insects with different bionetwork [J]. Microbiology, 2018, 87(1): 103−115. doi: 10.1134/S0026261718010137
[46]	PING L Y, BÜCHLER R, MITHÖFER A, et al. A novel Dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of N-acyl amino acids [J]. Environmental Microbiology, 2007, 9(6): 1572−1583. doi: 10.1111/j.1462-2920.2007.01279.x
[47]	MEDINA D, WALKE J B, GAJEWSKI Z, et al. Culture media and individual hosts affect the recovery of culturable bacterial diversity from amphibian skin [J]. Frontiers in Microbiology, 2017, 8: 1574. doi: 10.3389/fmicb.2017.01574
[48]	FERREIRA F S, HORVATH M B, TONDO E C. Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure [J]. Brazilian Journal of Microbiology, 2013, 44(3): 785−790. doi: 10.1590/S1517-83822013000300018