Optimization of <i>Bacillus velezensis</i> FJ17-4 Fermentation

LAN Chengzhong; LIN Xiong; GAN Lin; DAI Yuli; LIU Xiaofei; YANG Xiujuan; JIANG Junxi

doi:10.19303/j.issn.1008-0384.2022.010.013

Volume 37 Issue 10

Oct. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(10): 1335-1343

LAN C Z, LIN X, GAN L, et al. Optimization of Bacillus velezensis FJ17-4 Fermentation [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1335−1343 doi: 10.19303/j.issn.1008-0384.2022.010.013

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LAN C Z, LIN X, GAN L, et al. Optimization of Bacillus velezensis FJ17-4 Fermentation [J]. Fujian Journal of Agricultural Sciences，2022，37（10）：1335−1343 doi: 10.19303/j.issn.1008-0384.2022.010.013

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Optimization of Bacillus velezensis FJ17-4 Fermentation

doi: 10.19303/j.issn.1008-0384.2022.010.013

1.
Fujian Engineering Research Center for Green Pest Management, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
College of Agronomy, Jiangxi Agricultural University, Nanchang, Jiangxi　330045, China

Received Date: 2022-02-10
Rev Recd Date: 2022-09-15

Available Online: 2022-11-29

Publish Date: 2022-10-30

Abstract

Abstract

Objective Fermentation of Bacillus velezensis FJ17-4 known with a high inhibitory activity against several pathogens was optimized for potential application as a biocontrol agent. Methods Spectrometric measurement at OD₆₀₀ of the fermentation broth was used as the index for evaluation. Culture medium and conditions were optimized by single factor and orthogonal experiments. Bacterial quantity, pathogenic inhibition, and indoor potted control effect of the optimized fermentation product were determined. Results The optimized FJ17-4 fermentation used a medium that consisted of 12.5 g·L⁻¹ soybean meal, 5.0 g·L⁻¹ corn flour, and 12.5 g·L⁻¹ potassium dihydrogen phosphate at an initial pH of 7.0 to ferment a liquid loading (medium volume) of 50 mL with an inoculum size of 12.5% for 40 h at 30 ℃ in a 250 mL flask that rotated at a constant speed of 180 r·min⁻¹. The OD₆₀₀ of the optimized fermentation broth was 1.52 representing a cell load of 1.03×10¹⁰ cfu·mL⁻¹, which were 25.62% and 21.95%, respectively, higher than those prior to the optimization. The inhibition rate on the mycelial growth of Fusarium oxysporum f. sp. cucumerinum and the indoor potted control on cucumber fusarium wilt of the 50× optimized fermentation broth were 42.35% and 72.14%, representing 56.38% and 13.46% increases, respectively, over the original. Conclusion The FJ17-4 fermentation was significantly improved by the optimization. An operational cost reduction was also achieved.
- Bacillus velezensis,
- culture medium,
- fermentation conditions,
- optimization

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References(23)

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