Effects of Silicon on Growth and Functional Ingredients Accumulation of <i>Anoectochilus roxburghii</i>

DONG Qian; WANG Jiawang; HUANG Guoqiang

doi:10.19303/j.issn.1008-0384.2022.06.007

Volume 37 Issue 6

Jun. 2022

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Article Navigation > Fujian Journal of Agricultural Sciences > 2022 > 37(6): 734-740

DONG Q, WANG J W, HUANG G Q. Effects of Silicon on Growth and Functional Ingredients Accumulation of Anoectochilus roxburghii [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：734−740 doi: 10.19303/j.issn.1008-0384.2022.06.007

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DONG Q, WANG J W, HUANG G Q. Effects of Silicon on Growth and Functional Ingredients Accumulation of Anoectochilus roxburghii [J]. Fujian Journal of Agricultural Sciences，2022，37（6）：734−740 doi: 10.19303/j.issn.1008-0384.2022.06.007

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Effects of Silicon on Growth and Functional Ingredients Accumulation of Anoectochilus roxburghii

doi: 10.19303/j.issn.1008-0384.2022.06.007

1.
College of Food and Biological Engineering, Fujian Polytechnic Normal University, Fuqing, Fujian　350300, China
2.
Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-02-19
Accepted Date: 2022-02-19
Rev Recd Date: 2022-04-24

Available Online: 2022-05-21

Publish Date: 2022-06-28

Abstract

Abstract

Objective Effects of silicon on the growth and functional ingredients accumulation of Anoectochilus roxburghii were studied for the herbal crop quality improvement. Method Plants of Hong Xia, A. roxburghii (Wall.) Lindl, were supplied with concentrations of silicon at 0, 0.175, 0.350, 0.525, 0.700, and 0.875 mmol·L⁻¹ in a hydroponic experiment for 30 d. Effects of the treatments on the plant physiology and functional ingredients contents were monitored. Result Silicon addition promoted the growth of A. roxburghii. The relative growth rate under the supplementations of 0.175 to 0.700 mmol·L⁻¹ silicon was more than 7 times that of control. The chlorophyll content, chlorophyll index, and nitrogen balance index (NBI) all rose with increasing silicon to peak at 0.525 mmol·L⁻¹ and followed by a decline (P＜0.01), while the flavonoid decreased significantly to its lowest under 0.875 mmol·L⁻¹ (P＜0.01) and the peroxidase activity significantly increased more than 2 to 3 times that of control (P＜0.01). At high concentration of silicon (i.e., 0.700 mmol·L⁻¹), the accumulation of total flavonoids was 71.45%, and polysaccharides 116.65%, higher than those of control (P＜0.01). Conclusion Presence of silicon was beneficial for the growth of A. roxburghii. At an addition rate of 0.525 mmol·L⁻¹ during the vegetative growth period and 0.700 mmol·L⁻¹ in later stage, a maximized accumulation on flavonoids and polysaccharides could be realized.
- Silicon,
- Anoectochilus roxburghii (Wall.) Lindl.,
- flavonoids,
- polysaccharide,
- nitrogen balance index

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References(35)

References

[1]	梁永超, 张永春, 马同生. 植物的硅素营养 [J]. 土壤学进展, 1993, 21(3):7−14. LIANG Y C, ZHANG Y C, MA T S. Review of the studies on silicon nutrition of plants [J]. PROGRESS IN SOIL SCIENCE, 1993, 21(3): 7−14.(in Chinese)
[2]	EPSTEIN E. The anomaly of silicon in plant biology [J]. PNAS, 1994, 91(1): 11−17. doi: 10.1073/pnas.91.1.11
[3]	ARTHI V, SRIRAMACHANDRASEKHARAN M V, MANIVANNAN R, et al. Effect of silicon fertilization on agro-morphological traits of grand naine banana grown in typic ustifluvent soil [J]. International Journal of Plant & Soil Science, 2020: 38−46.
[4]	董倩, 黄国强, 王艳君, 等. 硅对果蔗组培腋芽苗增殖生长及相关指标的影响 [J]. 热带作物学报, 2018, 39(1):116−120. doi: 10.3969/j.issn.1000-2561.2018.01.018 DONG Q, HUANG G Q, WANG Y J, et al. Effects of silicon on proliferation, growth and relevant parameters of in vitro cultured fruit sugarcane axillary bud seedlings [J]. Chinese Journal of Tropical Crops, 2018, 39(1): 116−120.(in Chinese) doi: 10.3969/j.issn.1000-2561.2018.01.018
[5]	TRIPATHI P, NA C I, KIM Y. Effect of silicon fertilizer treatment on nodule formation and yield in soybean (Glycine max L. ) [J]. European Journal of Agronomy, 2021, 122: 126172. doi: 10.1016/j.eja.2020.126172
[6]	LIU C G, LU W K, MA Q N, et al. Effect of silicon on the alleviation of boron toxicity in wheat growth, boron accumulation, photosynthesis activities, and oxidative responses [J]. Journal of Plant Nutrition, 2017, 40(17): 2458−2467. doi: 10.1080/01904167.2017.1380817
[7]	HOSSEINI S A, NASERI RAD S, ALI N, et al. The ameliorative effect of silicon on maize plants grown in Mg-deficient conditions [J]. International Journal of Molecular Sciences, 2019, 20(4): 969. doi: 10.3390/ijms20040969
[8]	李炜蔷, 张逸, 石健, 等. 硅对大葱矿质元素吸收、分配特性及产量和品质的影响 [J]. 植物营养与肥料学报, 2016, 22(2):486−494. doi: 10.11674/zwyf.14466 LI W Q, ZHANG Y, SHI J, et al. Effects of silicon on mineral element uptake and distribution, yield and quality of Chinese spring onion [J]. Journal of Plant Nutrition and Fertilizer, 2016, 22(2): 486−494.(in Chinese) doi: 10.11674/zwyf.14466
[9]	ZHANG Y, CHEN H T, LIANG Y, et al. Comparative transcriptomic and metabolomic analyses reveal the protective effects of silicon against low phosphorus stress in tomato plants [J]. Plant Physiology and Biochemistry, 2021, 166: 78−87. doi: 10.1016/j.plaphy.2021.05.043
[10]	宁东峰, 梁永超. 硅调节植物抗病性的机理: 进展与展望 [J]. 植物营养与肥料学报, 2014, 20(5):1280−1287. doi: 10.11674/zwyf.2014.0525 NING D F, LIANG Y C. Silicon-mediated plant disease resistance: Advance and perspectives [J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(5): 1280−1287.(in Chinese) doi: 10.11674/zwyf.2014.0525
[11]	陈翠芳, 钟继洪, 李淑仪. 施硅对抑制植物吸收重金属镉的效应研究进展 [J]. 生态学杂志, 2007, 26(4):567−570. doi: 10.3321/j.issn:1000-4890.2007.04.021 CHEN C F, ZHONG J H, LI S Y. Research progress on inhibitory effects of silicon on cadmium absorption by plants [J]. Chinese Journal of Ecology, 2007, 26(4): 567−570.(in Chinese) doi: 10.3321/j.issn:1000-4890.2007.04.021
[12]	VACULÍK M, KOVÁČ J, FIALOVÁ I, et al. Multiple effects of silicon on alleviation of nickel toxicity in young maize roots [J]. Journal of Hazardous Materials, 2021, 415: 125570. doi: 10.1016/j.jhazmat.2021.125570
[13]	VOLETI S R, PADMAKUMARI A P, RAJU V S, et al. Effect of silicon solubilizers on silica transportation, induced pest and disease resistance in rice (Oryza sativa L. ) [J]. Crop Protection, 2008, 27(10): 1398−1402. doi: 10.1016/j.cropro.2008.05.009
[14]	HAN Y Q, WEN J H, PENG Z P, et al. Effects of silicon amendment on the occurrence of rice insect pests and diseases in a field test [J]. Journal of Integrative Agriculture, 2018, 17(10): 2172−2181. doi: 10.1016/S2095-3119(18)62035-0
[15]	THORNE S J, HARTLEY S E, MAATHUIS F J M. The effect of silicon on osmotic and drought stress tolerance in wheat landraces [J]. Plants, 2021, 10(4): 814. doi: 10.3390/plants10040814
[16]	沈廷明, 吴仲玉, 黄春情, 等. 金线莲化学成分、药理、组培及栽培研究进展 [J]. 海峡药学, 2016, 28(12):26−30. doi: 10.3969/j.issn.1006-3765.2016.12.010 SHEN T M, WU Z Y, HUANG C Q, et al. Study on Anoectochilus roxburghii's chemical constituents, pharmacodynamics, tissue culture and cultivation [J]. Strait Pharmaceutical Journal, 2016, 28(12): 26−30.(in Chinese) doi: 10.3969/j.issn.1006-3765.2016.12.010
[17]	陈育青, 林艺华, 邹毅辉, 等. 金线莲生药鉴定、活性成分影响因素及药理作用研究进展 [J]. 中成药, 2020, 42(8):2141−2144. doi: 10.3969/j.issn.1001-1528.2020.08.033 CHEN Y Q, LIN Y H, ZOU Y H, et al. Progress in Anoectochilus roxburghii's biochemical drug identification, factors affecting active ingredients and pharmacological effects [J]. Chinese Traditional Patent Medicine, 2020, 42(8): 2141−2144.(in Chinese) doi: 10.3969/j.issn.1001-1528.2020.08.033
[18]	薛梅花, 卢石孔, 翁文, 等. 不同种植方式对金线莲黄酮类化合物含量的影响 [J]. 福建分析测试, 2021, 30(1):33−36. doi: 10.3969/j.issn.1009-8143.2021.01.07 XUE M H, LU S K, WENG W, et al. Effects of planting methods on the content of flavonoids in Anoectochilus roxburghii [J]. Fujian Analysis & Testing, 2021, 30(1): 33−36.(in Chinese) doi: 10.3969/j.issn.1009-8143.2021.01.07
[19]	吴水华, 程伟青. 不同栽培方式对金线莲中多糖含量的影响 [J]. 现代中药研究与实践, 2016, 30(6):8−11. WU S H, CHENG W Q. Effects of different cultivation methods on polysaccharide content of different Anoectochilus roxburghii [J]. Research and Practice on Chinese Medicines, 2016, 30(6): 8−11.(in Chinese)
[20]	王建寰, 张文晋, 郎多勇, 等. 硅对盐胁迫下甘草非药用部位总黄酮、总皂苷积累动态的影响 [J]. 世界科学技术-中医药现代化, 2018, 20(7):1251−1255. WANG J H, ZHANG W J, LANG D Y, et al. Effects of silicon on the accumulation of total flavonoids and total saponins of non-medicinal parts of glvarrhiza uralensis fisch. under salt stress [J]. Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology, 2018, 20(7): 1251−1255.(in Chinese)
[21]	于涛, 张海楼, 隽英华, 等. 施肥模式对水稻稻瘟病抗性的影响 [J]. 江苏农业科学, 2014, 42(7):113−116. doi: 10.3969/j.issn.1002-1302.2014.07.038 YU T, ZHANG H L, JUAN Y H, et al. Effect of fertilization modes on resistance to rice blast [J]. Jiangsu Agricultural Sciences, 2014, 42(7): 113−116.(in Chinese) doi: 10.3969/j.issn.1002-1302.2014.07.038
[22]	何永美, 湛方栋, 祖艳群, 等. 大田条件下UV-B辐射对元阳梯田2个地方水稻品种硅、类黄酮和总酚含量的影响 [J]. 农业环境科学学报, 2013, 32(8):1500−1506. doi: 10.11654/jaes.2013.08.002 HE Y M, ZHAN F D, ZU Y Q, et al. Effects of UV-B radiation on the contents of silicon, flavonoids and total phenolic of two local rice varieties in Yuanyang Terrace under field conditions [J]. Journal of Agro-Environment Science, 2013, 32(8): 1500−1506.(in Chinese) doi: 10.11654/jaes.2013.08.002
[23]	CARVER T L W, ROBBINS M P, THOMAS B J, et al. Silicon deprivation enhances localized autofluorescent responses and phenylalanine ammonia-lyase activity in oat attacked by Blumeria graminis [J]. Physiological and Molecular Plant Pathology, 1998, 52(4): 245−257. doi: 10.1006/pmpp.1998.0149
[24]	杨开兴. 不同施肥模式对金线莲生长及生理特性的影响 [J]. 林业和草原机械, 2020, 1(4):34−38. YANG K X. Effects of different fertilization modes on growth and physiological characteristics of Anoectochilus [J]. Forestry and Grassland Machinery, 2020, 1(4): 34−38.(in Chinese)
[25]	应震, 杨燕萍, 周庄, 等. 不同基质和年份栽培对金线莲药用成分的影响 [J]. 浙江农业科学, 2020, 61(7):1356−1357. YING Z, YANG Y P, ZHOU Z, et al. Effects of different substrates and years on medicinal components of Anoectochilus roxburghii [J]. Journal of Zhejiang Agricultural Sciences, 2020, 61(7): 1356−1357.(in Chinese)
[26]	张文林, 任亚超, 张益文, 等. 不同光质LED光源对转基因杨树叶片Bt毒蛋白含量及叶绿素荧光参数的影响 [J]. 核农学报, 2016, 30(8):1639−1645. doi: 10.11869/j.issn.100-8551.2016.08.1639 ZHANG W L, REN Y C, ZHANG Y W, et al. Effect of different LED light qualities on bt toxic protein content and chlorophyll fluorescence of transgenic poplar leaves [J]. Journal of Nuclear Agricultural Sciences, 2016, 30(8): 1639−1645.(in Chinese) doi: 10.11869/j.issn.100-8551.2016.08.1639
[27]	常福辰, 陆长梅, 沙莎. 植物生物学实验[M]. 南京: 南京师范大学出版社, 2007.
[28]	张志信, 张铁, 赵保发, 等. 文山野生金线莲总黄酮及多糖含量测定 [J]. 时珍国医国药, 2009, 20(6):1362−1364. doi: 10.3969/j.issn.1008-0805.2009.06.032 ZHANG Z X, ZHANG T, ZHAO B F, et al. Determination of total flavonoids and polysaccharide of the wild anocetochilus plants in Wenshan prefecture [J]. Lishizhen Medicine and Materia Medica Research, 2009, 20(6): 1362−1364.(in Chinese) doi: 10.3969/j.issn.1008-0805.2009.06.032
[29]	熊蔚. 湿地草本植物叶硅含量特征及其与环境因子的关系[D]. 杭州: 杭州师范大学, 2016. XIONG W. Characteristics of silicon contents and their relationship to environmental factorsin wetland herbaceous plants[D]. Hangzhou: Hangzhou Normal University, 2016. (in Chinese)
[30]	李晓艳. 不同吸硅型植物硅同位素组成和营养元素分布特征[D]. 杭州: 浙江大学, 2013. LI X Y. Silicon isotope compositions and distribution patterns of Si and others nutritional elements in the different absorbing-silicon plants[D]. Hangzhou: Zhejiang University, 2013. (in Chinese)
[31]	曹逼力, 徐坤, 石健, 等. 硅对番茄生长及光合作用与蒸腾作用的影响 [J]. 植物营养与肥料学报, 2013, 19(2):354−360. doi: 10.11674/zwyf.2013.0211 CAO B L, XU K, SHI J, et al. Effects of silicon on growth, photosynthesis and transpiration of tomato [J]. Plant Nutrition and Fertilizer Science, 2013, 19(2): 354−360.(in Chinese) doi: 10.11674/zwyf.2013.0211
[32]	高臣, 刘俊渤, 常海波, 等. 硅对水稻叶片光合特性和超微结构的影响 [J]. 吉林农业大学学报, 2011, 33(1):1−4. GAO C, LIU J B, CHANG H B, et al. Effects of silicon on rice leaf photosynthesis and ultrastructure [J]. Journal of Jilin Agricultural University, 2011, 33(1): 1−4.(in Chinese)
[33]	李振海, 王纪华, 贺鹏, 等. 基于Dualex氮平衡指数测量仪的作物叶绿素含量估算模型 [J]. 农业工程学报, 2015, 31(21):191−197. doi: 10.11975/j.issn.1002-6819.2015.21.025 LI Z H, WANG J H, HE P, et al. Modelling of crop chlorophyll content based on Dualex [J]. Transactions of the Chinese Society of Agricultural Engineering, 2015, 31(21): 191−197.(in Chinese) doi: 10.11975/j.issn.1002-6819.2015.21.025
[34]	殷星, 侯振安, 冶军, 等. 应用多酚–叶绿素仪监测棉花氮素营养状况研究 [J]. 植物营养与肥料学报, 2021, 27(7):1198−1212. doi: 10.11674/zwyf.20617 YIN X, HOU Z N, YE J, et al. Application of polyphenol-chlorophyll meter to monitor cotton N nutrition status [J]. Journal of Plant Nutrition and Fertilizers, 2021, 27(7): 1198−1212.(in Chinese) doi: 10.11674/zwyf.20617
[35]	吴杏春, 陈裕坤, 李奇松, 等. 硅营养对UV-B辐射条件下水稻酚类代谢的影响 [J]. 中国农学通报, 2009, 25(24):225−230. WU X C, CHEN Y K, LI Q S, et al. Effects of silicon nutrition on phenolics metabolization of rice (Oryza sativa L. ) exposed to enhanced ultraviolet-B [J]. Chinese Agricultural Science Bulletin, 2009, 25(24): 225−230.(in Chinese)