Utilization of ITS Gene Barcodes for Identifying Plant-derived Functional Ingredients in Health Food Products

QIU Xibin

doi:10.19303/j.issn.1008-0384.2023.08.014

Volume 38 Issue 8

Aug. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(8): 989-1003

QIU X B. Utilization of ITS Gene Barcodes for Identifying Plant-derived Functional Ingredients in Health Food Products [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：989−1003 doi: 10.19303/j.issn.1008-0384.2023.08.014

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QIU X B. Utilization of ITS Gene Barcodes for Identifying Plant-derived Functional Ingredients in Health Food Products [J]. Fujian Journal of Agricultural Sciences，2023，38（8）：989−1003 doi: 10.19303/j.issn.1008-0384.2023.08.014

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Utilization of ITS Gene Barcodes for Identifying Plant-derived Functional Ingredients in Health Food Products

doi: 10.19303/j.issn.1008-0384.2023.08.014

QIU Xibin

Fujian Inspection and Research Institute for Product Quality/National Quality Supervision and Testing Center for Processed Food (FuZhou) , Fuzhou, Fujian　350002, China

Received Date: 2022-09-15
Rev Recd Date: 2023-05-25

Available Online: 2023-08-16

Publish Date: 2023-08-28

Abstract

Abstract

Objective A molecular biology identification method to detect common plant-derived functional ingredients in health food products using genetic barcodes was developed. Method By means of retrospective investigation, 5 common health food raw materials in China, i.e., American ginseng (Panax quinquefolius), Dendrobium candidum, rhodiola (Rhodiola nobilis), wolfberries (Lycium barbarum L.), and ginseng (Radix Ginseng), were firstly tested for functional ingredients using the best extraction method on the target DNA. A stable, efficient, and accurate PCR reaction system was constructed with primer sequence designed based on the conservative characteristics of ITS2 internal transcribed spacer and components of each substance adjusted and optimized. Molecular identification on health food products of various forms was tested using the developed method. Result The innovative application of a modified CTAB method afforded successful extraction of the target DNA in health food materials as well as products at commonly formulated dosages. The PCR amplified with the detection sensitivity on nucleic acids of American ginseng, D. candidum, and rhodiola reached 1 ng·μL⁻¹, and of wolfberries and ginseng 0.1 ng·μL⁻¹. Conclusion By using the ITS gene barcodes obtained through the DNA extraction and PCR amplification, the key functional ingredients from plant materials such as American ginseng, ginseng, D. candidum, wolfberries, and rhodiola rosea could be identified for authentication of health food products in the forms of pills, tablets, and concoctions.
- Gene barcode,
- health food,
- plant-derived functional ingredients,
- PCR

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References(21)

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