Isolation and Enzymatic Activity of Cellulose-degrading Bacteria

ZHANG Hui; ZHAO Ke; LIN Chenqiang; LIN Rongbin; CHEN Longjun; CHEN Jichen

doi:10.19303/j.issn.1008-0384.2023.09.014

Volume 38 Issue 9

Sep. 2023

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Article Navigation > Fujian Journal of Agricultural Sciences > 2023 > 38(9): 1117-1123

ZHANG H, ZHAO K, LIN C Q, et al. Isolation and Enzymatic Activity of Cellulose-degrading Bacteria [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1117−1123 doi: 10.19303/j.issn.1008-0384.2023.09.014

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ZHANG H, ZHAO K, LIN C Q, et al. Isolation and Enzymatic Activity of Cellulose-degrading Bacteria [J]. Fujian Journal of Agricultural Sciences，2023，38（9）：1117−1123 doi: 10.19303/j.issn.1008-0384.2023.09.014

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Isolation and Enzymatic Activity of Cellulose-degrading Bacteria

doi: 10.19303/j.issn.1008-0384.2023.09.014

1.
Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 　350013, China
2.
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

Received Date: 2022-05-23
Rev Recd Date: 2023-07-26

Available Online: 2023-10-25

Publish Date: 2023-09-28

Abstract

Abstract

Objective Bacteria capable of effectively degrading spent straws were isolated, and conditions for the enzymatic reaction analyzed. Method On a medium containing sodium carboxymethyl cellulose and Congo red, potential strains capable of decomposing fibrous material were isolated from sampled forest soil and compost. Enzymatic activities of the isolates were examined, and conditions determined. Result Bacillus licheniformis was identified as the strain with the greatest carboxymethyl cellulase activity. It demonstrated a filter paper degrading activity of 14.21 U·mL⁻¹, a xylanase activity of 24.03 U·mL⁻¹, and an ability to decompose 21.2% of corn straws. On corn straws, the selected isolate was observed to show the maximum digestion with an overall activity up to 48.63 U·mL⁻¹ by using KNO₃ for nitrogen source at pH 7 and 30 ℃. Conclusion B. licheniformis was a cellulose-degrading bacterium that could be used to decompose spent corn straws for waste disposal.
- Cellulose-degrading bacteria,
- corn straw,
- enzymatic properties

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References(27)

References

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