Studies on the Changes of Ureide Content in Adzuki Bean (vigna angularis)

Changes of ureide,allantoin,allantoic acid content in leaves and nodules of adzuki bean (Vigna angularis) and effect of some exogenous compounds on the ureide content in seedlings were inverstigated.Diurnal variation of ureide content occured in leaves and nodules.Allantoic acid content was higher than that of allantoin in the nodules.Diurnal variation in ureide,allantoin,allantoic acid content in leaves displayed a peak at 16:00 which occured one hour later than that in the nodules.However,diurnal fluctuation patterns of allantoin content was similar to that of allantoic acid content in both the leaves and the nodules.Several compounds included in the growth medium were tested for their ability to affect the synthesis of allantoin,allantoic acid in germinating seedlings.Mn2+ showed no appreciable effect on the allantoinase activity and allantoin,allantioc acid synthesis,but Cu2+inhibited the allantoinase activity.However,KT (l-10ppm) and BA(l-25ppm) did not have any effect on the allantoinase activity.ZT(l-25ppm)had slight effect on the ureide content.ABA (lppm) repressed the germination but did not affect the ureide content.