Regeneration of Bulblets from Tissue Culture of Oriental Hybrid Lilium,"Sorbonne"

An efficient tissue culture was developed using the bulb scale,scale′s leave segments and bulblet enlargement of the Oriental Hybrid Lilium,"Sorborne".Results showed that(a) the appropriate induction medium for regeneration of the bulblet from the bulb scale and scale′s leave segments was MS+ 0.2 mg·L-1 6-BA+NAA 0.2 mg·L-1 +0.1 mg·L-1 2,4-D;(b) there was no significant difference in quantity,induced rate and fresh weight of the regenerated bulblets resulting from the 3 putting methods;(c) the induction rate of the regenerated bulblet of scale’s leave segments was the highest in the basal,followed by the middle section,and gradually decreased from bottom to top;(d) 30-60 g·L-1 was the optimal sucrose concentration for inducing the bulblet regeneration;(e) for bulblet enlargement,the sucrose concentration should be lower than 90 g·L-1 when treated in the dark,and 120 g·L-1 when exposed under scattered light.