Cloning and expression analysis of RhMAX2A gene in Rosa hybrid

LI Shasha; DU Gaoqi; LI Xuejiao; GUAN Wenling; MENG Jing

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LI S S, DU G Q, LI X J, et al. Cloning and expression analysis of RhMAX2A gene in Rosa hybrid [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

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LI S S, DU G Q, LI X J, et al. Cloning and expression analysis of RhMAX2A gene in Rosa hybrid [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

LI S S, DU G Q, LI X J, et al. Cloning and expression analysis of RhMAX2A gene in Rosa hybrid [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

Citation:

LI S S, DU G Q, LI X J, et al. Cloning and expression analysis of RhMAX2A gene in Rosa hybrid [J]. Fujian Journal of Agricultural Sciences，2024，39（2）：1−11

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Cloning and expression analysis of RhMAX2A gene in Rosa hybrid

1.
College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan　650201, China

Received Date: 2023-08-21
Accepted Date: 2024-02-27
Rev Recd Date: 2024-01-26

Available Online: 2024-03-28

Abstract

Abstract

: Objective To study the biological function and the transduction mechanism that regulates lateral branching of the RhMAX2A gene in Rosa, the cDNA sequence of RhMAX2A was cloned and was analysed for sequence characteristics and its expression in different tissues and after decapitation. Methods The cDNA sequence of RhMAX2A gene was cloned from Rosa hybrida ‘Dianhong’ by RT-PCR and the bioinformatics analysis was conducted. The subcellular location of RhMAX2A was analyzed by transient transformation of PC1300s-RhMAX2A-GFP in tobacco leaves. The expression levels of RhMAX2A in different tissues and the treatment of decapitation was detected by real-time quantitative PCR (qRT-PCR). Results The cDNA sequence of RhMAX2A gene (GenBank accession number: OP055810 ) was 1030 bp in length, encoding 246 amino acids. The calculated chemical molecular formula of RhMAX2A protein is C₂₉₁₀H₄₇₉₃N₁₀₂₉O₁₂₄₄S₂₁₀, with 27.35 kD in molecular mass and the total atomic weight is 3909. The instability coefficient of this protein is 53.07, with fat coefficient-106.30 and GRAVY value 0.049, indicating that it is an unstable hydrophilic protein. The secondary structure of RhMAX2A protein is mainly composed of α-helix and random coil, and RhMAX2A is a presumed F-box domain, belonging to the α/β hydrolase family. Homologous sequence alignment and phylogenetic tree analysis showed that the amino acid sequence of RhMAX2A (OP055810) had the highest similarity with that of Rosa chinensis Old Blush (XP_024283944.1), followed by Fragaria vesca subsp. vesca (XP_004287076.1) of the same subfamily, which were closely related. The subcellular localization results showed that the encoded protein of RhMAX2A was located in the nucleus. The results of qRT-PCR indicated that RhMAX2A gene was expressed in roots, axillary buds and nodes, with the highest expression level in roots. The decapitation treatment significantly upregulated the expression of RhMAX2A in roots and axillary buds. Conclusion The RhMAX2A gene was successfully cloned from Rosa hybrida ‘Dianhong’, which encodes a protein that plays a role in the nucleus, mainly expressed in roots and axillary buds, and could be upregulated by decapitation.
- Rosa hybrida,
- MAX2A gene,
- gene cloning,
- subcellular localization,
- expression analysis

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References(31)

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