Cloning and Construct of Over-expression Vector of <em>IPA1</em> cDNA from Nipponbare

LIAN Ling; HE Wei; CAI Qiu-hua; XU Hui-bin; ZHU Yong-sheng; ZHANG Jian-fu; XIE Hua-an

Volume 27 Issue 9

Nov. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(9): 913-918

LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918.

Citation:

LIAN Ling, HE Wei, CAI Qiu-hua, XU Hui-bin, ZHU Yong-sheng, ZHANG Jian-fu, XIE Hua-an. Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 913-918.

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Cloning and Construct of Over-expression Vector of IPA1 cDNA from Nipponbare

LIAN Ling^1,1,2,3,4,5,
HE Wei^1,2,3,4,5,
CAI Qiu-hua^1,2,3,4,5,
XU Hui-bin^1,2,3,4,5,
ZHU Yong-sheng^1,2,3,4,5,
ZHANG Jian-fu^{1,2,3,4,5
,
,},
XIE Hua-an^{1,2,3,4,5
,
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China;
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China;
3.
Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;
4.
National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China;
5.
South China Bases of National Key Laboratory of Hybrid Rice for China, Fuzhou, Fujian 350003, China

Received Date: 2012-06-01
Rev Recd Date: 2012-07-09
Publish Date: 2012-09-30

Abstract

Abstract

A cDNA encoding IPA1 was isolated from Nipponbare by RT-PCR, sequencing results showed that the sequence consists of 1 257 bp with a complete CDS and encodes a protein of 418 amino acids. In addition, the prediction results indicated that molecular weight of target protein was 42.51 kD and theoretical isoelectric point was 9.37. The plant expression vector was constructed by ligating the cDNA fragment into expression vector pHI, then the recombinant plasmid pHI-IPA1 was confirmed by restriction enzyme digestion analysis with SmaI、SacI, and further verified by DNA sequencing. Vector plasmid was transformed into Agrobacterium tumefaciens, which will lay foundation for rice genetic transformation.
- rice,
- IPA1,
- cDNA cloning,
- vector construction

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References(2)

References

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