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CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, WEI Hong, ZHUANG Xiang-sheng, LIU Yu-tao, YAN Shan, ZHOU Lun-jiang. Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 941-944.
Citation:
CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, WEI Hong, ZHUANG Xiang-sheng, LIU Yu-tao, YAN Shan, ZHOU Lun-jiang. Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 941-944.
CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, WEI Hong, ZHUANG Xiang-sheng, LIU Yu-tao, YAN Shan, ZHOU Lun-jiang. Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 941-944.
Citation:
CHEN Ru-jing, WU Xue-min, CHE Yong-liang, WANG Long-bai, WEI Hong, ZHUANG Xiang-sheng, LIU Yu-tao, YAN Shan, ZHOU Lun-jiang. Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian[J]. Fujian Journal of Agricultural Sciences, 2012, 27(9): 941-944.
Institute of Animal Husbandry and Veterinary Medicine/Fujian Animal Diseases Control Technology Development Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China
The 3D gene of porcine kobuvirus was amplified by RT-PCR from tissues of piglets association with diarrhea from Fujian and then sequenced. The results showed that the porcine kobuvirus 3D gene was 1 407 bp in length, coding an open reading frame (ORF) containing 468 amino acids.Compared with the published PKV 3D genes by the bioinformatics software, the sequenced gene shared the highest homogeneity with SH-W-CHN/2010/China strain of 93.6%. In the phylogenetic tree, the porcine kobuvirus Fujian strain was at the same branch with other strains isolated in China while the Hungarian isolates share another branch, which indicated that the porcine kobuvirus had its own evolution status in different continents.
REUTER G, BOROS A, PANKOVICS P. Kobuviruses-a comprehensive review [J]. Rev Med Virol, 2011, 21(1):32-41.
[2]
REUTER G, BOLDIZS R A, PANKOVICS P. Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae [J]. Arch Virol, 2009, 154(1):101-108.
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