Isolation, identification, biological characteristics, and indoor drug screening of the pathogenic bacteria of <i>Atractylodes macrocephala</i> root rot disease

GU Qingyi; ZHANG Yaozhou; Wu Xiaoya; HUANG Yaqin; QIAO Xinrong; SHEN Jun

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GU Q Y, ZHANG Y Z, Wu X Y, et al. Isolation, identification, biological characteristics, and indoor drug screening of the pathogenic bacteria of Atractylodes macrocephala root rot disease [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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GU Q Y, ZHANG Y Z, Wu X Y, et al. Isolation, identification, biological characteristics, and indoor drug screening of the pathogenic bacteria of Atractylodes macrocephala root rot disease [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−9

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Isolation, identification, biological characteristics, and indoor drug screening of the pathogenic bacteria of Atractylodes macrocephala root rot disease

Received Date: 2023-10-25
Rev Recd Date: 2024-01-12

Available Online: 2024-03-28

Abstract

Abstract

: Objective In order to identify the pathogenic genera of Atractylodes macrocephala root rot disease in Xinyang, Henan Province, study their biological characteristics, and screen for Botanical fungicides that can be used for prevention and control. Method Morphological and multi gene analysis were used to identify the pathogens, and the biological characteristics of the pathogens were studied. Result The pathogen has white hyphae, oval shaped spores with slightly pointed ends, and large conidia with 3-5 septa and sizes of 7-10μm×3-4μm. Small conidia with 2 compartments or no compartments, size 3-5μm × 1-2μm. Multi gene joint analysis showed that the pathogen had high homology with Fusarium Fujikuroi. Combining morphological characteristics and molecular biology analysis, the pathogen of Atractylodes macrocephala root rot in Xinyang was identified as Fusarium Fujikuroi. This is the first report of Fusarium Fujikuroi causing Atractylodes macrocephala root rot. Research on biological characteristics shows that the optimal cultivation conditions for this pathogen are temperature 28 ℃, pH 7, sucrose as the optimal carbon source for growth, and potassium nitrate as the optimal nitrogen source for growth. Among the three botanical fungicides, the EC₅₀ of 0.3% eugenol soluble agent is 6.906 mg/L, which is higher than other tested agents and has the strongest toxicity to pathogens.. Conclusion The pathogen of Atractylodes macrocephala root rot in Xinyang, Henan Province is Fusarium fuciformis. The optimal cultivation conditions are temperature 28 ℃, pH 7, sucrose as the carbon source for growth, and potassium nitrate as the nitrogen source for growth. The research results provide a basis for the scientific prevention and control of root rot disease of Atractylodes macrocephala in Xinyang area.
- Atractylodes macrocephala,
- root rot,
- Fusarium Fujikura,
- Botanical fungicides

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References(36)

References

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