Determination of enrofloxacin and ciprofloxacin residues in aquatic products by Surface-enhanced Raman Spectroscopy

LIU Wen-Jing; PAN Wei; LIN Hui-Zhen; CHEN Hong-Ju; YAN Shao-De

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LIU W - J, PAN W, LIN H - Z, et al. Determination of enrofloxacin and ciprofloxacin residues in aquatic products by Surface-enhanced Raman Spectroscopy [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

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LIU W - J, PAN W, LIN H - Z, et al. Determination of enrofloxacin and ciprofloxacin residues in aquatic products by Surface-enhanced Raman Spectroscopy [J]. Fujian Journal of Agricultural Sciences，2024，39（X）：1−8

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Determination of enrofloxacin and ciprofloxacin residues in aquatic products by Surface-enhanced Raman Spectroscopy

1.
Institute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences/Fujian Key Laboratory of Agro-products Qualitiy & Safety, Fuzhou, Fujian　350003, China
2.
Xiamen PERSer Nanotechnology Co., Ltd, Xiamen, Fujian　361021, China
3.
Xiamen Assessment Service Center of Quality and Technology, Xiamen, Fujian　361021, China

Received Date: 2023-06-06
Rev Recd Date: 2023-12-07

Available Online: 2024-03-28

Abstract

Abstract

: Objective A rapid method for detection of Enrofloxacin (ENR)and ciprofloxacin (CIP) residues in vegetables and fruits by Surface-enhanced Raman spectroscopy (SERS) was established. Method The best detection conditions were obtained by screening of enhancement reagent, optimizing extraction solvents and reaction time using fruit and vegetable products as the research objects.The silver nanoparticles with a particle size of 50±5 nm was used as the SERS enhancement reagent, 200 g·L⁻¹ NaCl solution was used as the extraction solution, and the results were interpreted within 3 min. The linear regression equation of peak intensity and concentration was established, and the linear relationship and detection limit were established to evaluate the method. Result The results indicate that, the Raman spectrograms peaks of ENR and CIP are 532 cm⁻¹、552 cm⁻¹、651 cm⁻¹、737 cm⁻¹and 785 cm⁻¹, of which 532 cm⁻¹ is caused by C-N bending vibration, and 552 cm⁻¹ is the out-of-plane bending vibration, 651 cm⁻¹ is attributed to C-N, C-C-F out-of-plane bending vibration and C-C=O in-plane bending vibration .737 cm⁻¹ is attributed to the stretching vibration of C-H on the benzene ring, C-C=O out-of-plane bending vibration, C-C-N bending vibration and C-H, C-N stretching vibration, and 785 cm⁻¹ is attributed to C-H, C-N out-of-plane bending vibration. By quantifying the characteristic peak(737 cm⁻¹), the Raman intensity and the concentration have a good linear relationship within 10 ng·mL⁻¹～200 ng·mL⁻¹, and R² ＞ 0.96, the recovery rate was 78.4%～106.7%, the RSD was 2.1%～6.7%, and the method detection limit was 10 μg·kg⁻¹. Conclusion The method is accurate, rapid, stable, and can achieve ENR and CIP in aquatic products in the field for rapid screening and detection.
- surface-enhanced Raman spectroscopy,
- enrofloxacin,
- ciprofloxacin,
- rapid detection

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References(28)

References

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