Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!
SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, YANG Wei-xing, HUANG Yu. Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 11-13.
Citation:
SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, YANG Wei-xing, HUANG Yu. Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 11-13.
SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, YANG Wei-xing, HUANG Yu. Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 11-13.
Citation:
SHI Shao-hua, CHENG Long-fei, CHEN Hong-mei, FU Guang-hua, YANG Wei-xing, HUANG Yu. Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1[J]. Fujian Journal of Agricultural Sciences, 2009, 24(1): 11-13.
An RT-PCR method was developed to detect virulent strains of waterfowl-origin avian paramyxovirus type 1(vwAPMV-1).The primers were designed according to the sequences of the fusion protein gene of vwAPMV-1s available at the GenBank.The result showed that the 400 bp of specific fragments were amplified in vwAPMV-1.However,negative results were obtained from Pasteurella multocide,EDS,DEV,IBDV,H9N2 influenza virus,DHV-1 and low virulent strains of waterfowl-origin avian paramyxovirus type 1.And,the 10pg of vwAPMV-1 RNA was amplified by the sensitivity test for the RT-PCR.The results indicated that the RT-PCR could be used for vwAPMV-1 detection.
DownLoad: