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SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu. Development of PCR assay for detecting avian Pasteurella multocida[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 201-204.
Citation:
SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu. Development of PCR assay for detecting avian Pasteurella multocida[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 201-204.
SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu. Development of PCR assay for detecting avian Pasteurella multocida[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 201-204.
Citation:
SHI Shao-hua, CHENG Long-fei, FU Guang-hua, CHEN Hong-mei, WAN Chun-he, CHEN Zhen, PENG Chun-xiang, HUANG Yu. Development of PCR assay for detecting avian Pasteurella multocida[J]. Fujian Journal of Agricultural Sciences, 2009, 24(3): 201-204.
To establish a specific and sensitive detection methodology for avian Pasteurella multocida infection,PCR was applied.The 460 bp of the specific fragments was amplified from avian P.multocida genome.None could be obtained from Escherichia coli,Riemerella anatipestifer,Salmonella,Staphyloccocus aureus,Avian paramyxovirus type 1,Muscovy duck retrovirus or genome of duck.The sensitivity test indicated that a minimum of 1ngμL-1 of avian P.multocida genome DNA could be detected by the newly developed PCR method.
ROCKE T E,SMITH S R,MIYAMOTO A,et al.A serotype-specific polymerase chain reaction for identification of Pasteurella multocida serotype 1[J].Avian Diseases,2002,46(2):370-377.
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