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SU Jun, LIN Mei-kun, DU Lin, HONG Hai-qiang, WANG Feng. Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 1-7.
Citation:
SU Jun, LIN Mei-kun, DU Lin, HONG Hai-qiang, WANG Feng. Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 1-7.
SU Jun, LIN Mei-kun, DU Lin, HONG Hai-qiang, WANG Feng. Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 1-7.
Citation:
SU Jun, LIN Mei-kun, DU Lin, HONG Hai-qiang, WANG Feng. Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice[J]. Fujian Journal of Agricultural Sciences, 2010, 25(1): 1-7.
Application of multigene expression vector containing transformation-competent artificial chromosome and agrobacterium-mediated transformation for studying their effects on rice
By using a transformation-competent artificial chromosome vector pYLTAC747H,we constructed four genes relating to assimilation translocation,including OsSUT5,OsMST4,OsCIN3 and OAAT in the expression vector,pTAC747H-OAAT-SUT5-CIN3-MST4.The entire inserted fragment was approximately 30 kb.By the agrobacterium-mediated transformation,7 independent clones were obtained.PCR detection showed that 4 target genes and one marker gene had been integrated into the rice genome.
LIN L,LIU Y G,XU X P,et al.Efficient linkin gand transfer of multiple genes by A multigene assembly and transformation vector system[J].PNAS,2003,100(3):5962-5967.
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