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The relationships and differences among the species,Cucurbita,pepo and ficifofia (Cucurbitaceae:Cucurbita),were examined using data from the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA. The ITS sequences were obtained from Cucurbita,pepo and ficifofia 3 species. The results showed that,within Cucurbita,ITS1 was 188-191bp (C. Cucurbita,C. pepo and C. ficifofia) and ITS2 ranged from 251-263bp (C. Cucurbita,C. pepo and C. ficifofia). There were 33 variable positions (17.55%) and 24 informative sites (12.77%) within Cucurbita found in the ITS1 sequences,while 45 variable positions (17.93%) and 31 informative sites (12.35%) found in the ITS2 sequences.The G+C content of ITS1 was 51.60% to 54.50% and that of ITS2 ranged from 53.78% to 56.98%.The resulting trees from the ITS1 and ITS2 sequences showed that the relationship between C. Cucurbita and C. pepo was closer than that between C. Cucurbita and C. ficifofia.
HSIAO C,CHATTERTON N J,ASAY K H,et al.Phylogenetic relationships of the monogenomic species of the wheat tribe,Triticeae(Poaceae),inferred from nuclear rDNA (internal transcribed spacer) sequences[J].Genome,1995,38 (2):211-223.
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