Quantitative detection of ALV-J by fluorescence RT-PCR

LIN Su; ZHU Chun-hua; CHEN Zhen; LIU Bin-qiong; SHI Shao-hua; JIANG Bin; CAI Guo-zhang; LIN Yu; HUANG Yu

Volume 25 Issue 3

Nov. 2011

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Article Navigation > Fujian Journal of Agricultural Sciences > 2010 > 25(3): 289-293

LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.

Citation:

LIN Su, ZHU Chun-hua, CHEN Zhen, LIU Bin-qiong, SHI Shao-hua, JIANG Bin, CAI Guo-zhang, LIN Yu, HUANG Yu. Quantitative detection of ALV-J by fluorescence RT-PCR[J]. Fujian Journal of Agricultural Sciences, 2010, 25(3): 289-293.

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Quantitative detection of ALV-J by fluorescence RT-PCR

1.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

Received Date: 2010-03-03
Rev Recd Date: 2010-04-07
Publish Date: 2010-06-15

Abstract

Abstract

Avian leukosis is one of the main chicken tumor diseases.It causes considerable economic loss for poultry farmers.A new,rapid diagnostic mehtod applying the real-time florescence quantitative polymerase chain reaction(PT-PCR)was developed for detecting the avian leukosis virus subgroup J(ALV-J).A set of subgroup J special primers for gp85 gene was used.Validations on repeatability,specificity and sensitivity of the methodology showed its potential applicability for clinical diagnosis of ALV-J.
- avian leukosis,
- RT-PCR,
- gp85 gene

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References(6)

References

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